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公开(公告)号：EP1311707A2
公开(公告)日：2003-05-21
申请号：EP01964178.6
申请日：2001-08-17
IPC分类号： C12Q1/68
CPC分类号： H01J49/0036 , Y10T436/24 , Y10T436/25125
摘要： The present invention makes use of unique tags of a specific biopolymer that can be exploited for determining the concentration the biopolymer in crude solutions. In preferred embodiments the biopolymer is either a protein or a polynucleotide. Particularly, the invention provides a method for the determination and quantitation of biomolecules in crude mixtures by way of a separatioln technique in combination with mass spectroscopy. In one general embodiment, a target biomolecule is selected for analysis and an analog thereof is generated. Peak area integration of the peptide pairs provides a direct measure for the amount of target protein in the crude solution.
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公开(公告)号：EP1311707B1
公开(公告)日：2008-10-15
申请号：EP01964178.6
申请日：2001-08-17
IPC分类号： C12Q1/68
CPC分类号： H01J49/0036 , Y10T436/24 , Y10T436/25125
摘要： The present invention makes use of unique tags of a specific biopolymer that can be exploited for determining the concentration the biopolymer in crude solutions. In preferred embodiments the biopolymer is either a protein or a polynucleotide. Particularly, the invention provides a method for the determination and quantitation of biomolecules in crude mixtures by way of a separatioln technique in combination with mass spectroscopy. In one general embodiment, a target biomolecule is selected for analysis and an analog thereof is generated. Peak area integration of the peptide pairs provides a direct measure for the amount of target protein in the crude solution.
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