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    EXPRESSION SYSTEM FOR ALTERED EXPRESSION LEVELS
    9.
    发明授权
    EXPRESSION SYSTEM FOR ALTERED EXPRESSION LEVELS 失效
    衍生自拟OMAS产碱杆菌的脂酶调节CASCADE表达系统

    公开(公告)号：EP0918851B1

    公开(公告)日：2004-10-13

    申请号：EP97938360.1

    申请日：1997-08-15

    申请人： GENENCOR INTERNATIONAL, INC.

    发明人： GERRITSE, Gijsbert ,  QUAX, Wilhelmus, J.

    IPC分类号： C12N15/00

    CPC分类号： C07K14/21 ,  C12N9/20 ,  C12N15/78

    摘要： A new expression system is provided which comprises component(s) of a lipase regulation cascade. The lipase regulation cascade as disclosed herein includes a kinase, a DNA binding regulator, a polymerase, a promoter, an upstream activating sequence, and secretion factors. Plasmids and transformed cells are also provided as well as methods of transforming host cells using the plasmids. Further, there is provided a kinase that can regulate the expression of a protein, a DNA binding regulator that can regulate the expression of a protein, a Pseudomonas alcaligenes polymerase, a Pseudomonas alcaligenes sigma 54 promoter, a Pseudomonas alcaligenes upstream activating sequence, the Pseudomonas alcaligenes secretion factors XcpP, XcpQ, XcpR, XcpS, XcpT, XcpU, XcpV, XcpW, XcpX, XcpY, XcpZ and the xcp regulators OrfV, OrfX.

    EXPRESSION SYSTEM FOR ALTERED EXPRESSION LEVELS
    10.
    发明公开
    EXPRESSION SYSTEM FOR ALTERED EXPRESSION LEVELS 失效
    衍生自拟OMAS产碱杆菌的脂酶调节CASCADE表达系统

    公开(公告)号：EP0918851A2

    公开(公告)日：1999-06-02

    申请号：EP97938360.0

    申请日：1997-08-15

    申请人： GENENCOR INTERNATIONAL INC.

    发明人： GERRITSE, Gijsbert ,  QUAX, Wilhelmus, J.

    IPC分类号： C07K14 ,  C12N9 ,  C12N15

    CPC分类号： C07K14/21 ,  C12N9/20 ,  C12N15/78

    摘要： An expression system is provided which comprises component(s) of a lipase regulation cascade. The lipase regulation cascade as disclosed herein includes a kinase, a DNA binding regulator, a polymerase, a promoter, an upstream activating sequence, and secretion factors. Plasmids and transformed cells are also provided as well as methods of transforming host cells using the plasmids. Further, there is provided a kinase that can regulate the expression of a protein, a DNA binding regulator that can regulate the expression of a protein, a Pseudomonas alcaligenes polymerase, a Pseudomonas alcaligenes sigma 54 promoter, a Pseudomonas alcaligenes upstream activating sequence, the Pseudomonas alcaligenes secretion factors XcpP, XcpQ, XcpR, XcpS, XcpT, XcpU, XcpV, XcpW, XcpX, XcpY, XcpZ and the xcp regulators OrfV, OrfX.