公开(公告)号：EP0178764A1

公开(公告)日：1986-04-23

申请号：EP85306061.4

申请日：1985-08-27

申请人： GENEX CORPORATION

发明人： Anderson, David Martin

IPC分类号： C12N15/00 ,  C12P21/00 ,  C12N1/20

CPC分类号： C12N9/6481 ,  C12N9/1014 ,  C12N15/67

摘要： This invention provides a method of stabilizing a host microorganism / expression vector system for large scale production of a protein which comprises transforming an auxotrophic strain of said microorganism, having at least one mutation in the tryptophan operon such that the cells are unable to produce sufficient tryptophan to survive or grow, with a recombinant vector which comprises (a) a gene which encodes a protein of interest and is under the control of regulatory sequences for expression and (b) one or more functional trp genes, under the control of regulatory sequences for expression, which at least complement the mutation(s) in the tryptophan operon of the host cells thereby enabling the said host cells to synthesize tryptophan.

摘要翻译： 本发明提供稳定用于大规模生产，它包括变换为营养缺陷型。所述微生物的菌株的蛋白的宿主微生物/表达载体系统的方法，具有在所检查的色氨酸操纵子的至少一个突变做的细胞不能产生足够的色氨酸 生存或生长，与包含重组载体（a）中编码的目的蛋白质，并且是表达和（b）一个或多个官能trp启动基因的调控序列的控制下，用于调节序列的控制下的基因 表达，这至少在宿主细胞从而使得所述宿主细胞合成色氨酸的色氨酸操纵子补充突变。

公开(公告)号：EP0182636A3

公开(公告)日：1988-01-07

申请号：EP85308361

申请日：1985-11-15

申请人： GENEX CORPORATION

发明人： Anderson, David Martin ,  McGuire, Jeffrey Clark

IPC分类号： C12N15/00 ,  C12N09/64

CPC分类号： C12N9/6481 ,  C12N15/71

摘要： A method and vectors for high level expression of genes in bacteria are disclosed. According to one aspect, the present invention provides a method for expressing a gene coding for a desired protein in a bacterium at elevated levels of expression, which comprises ligating at a site adjacent to the translation termination codon of a structural gene for the desired protein, a terminal mRNA sequence from a gene coding for a stable mRNA coding for a bacterial protein; situating the ligated gene so formed in a vector that is replicable in the bacterium such that the gene is operably linked to a transcription promoter; transforming the bacterium with the vector containing the gene; and expressing the gene in the transformed bacterium.

公开(公告)号：EP0182636A2

公开(公告)日：1986-05-28

申请号：EP85308361.6

申请日：1985-11-15

申请人： GENEX CORPORATION

发明人： Anderson, David Martin ,  McGuire, Jeffrey Clark

IPC分类号： C12N15/00

CPC分类号： C12N9/6481 ,  C12N15/71

摘要： A method and vectors for high level expression of genes in bacteria are disclosed. According to one aspect, the present invention provides a method for expressing a gene coding for a desired protein in a bacterium at elevated levels of expression, which comprises ligating at a site adjacent to the translation termination codon of a structural gene for the desired protein, a terminal mRNA sequence from a gene coding for a stable mRNA coding for a bacterial protein; situating the ligated gene so formed in a vector that is replicable in the bacterium such that the gene is operably linked to a transcription promoter; transforming the bacterium with the vector containing the gene; and expressing the gene in the transformed bacterium.

摘要翻译： 公开了一种在细菌中高水平表达基因的方法和载体。 根据一个方面，本发明提供了一种用于在升高的表达水平的细菌中表达编码所需蛋白质的基因的方法，其包括在与所需蛋白质的结构基因的翻译终止密码子相邻的位点连接， 来自编码细菌蛋白质的稳定mRNA的基因的末端mRNA序列; 将如此形成的连接基因置于可在细菌中可复制的载体中，使得该基因与转录启动子可操作地连接; 用含有该基因的载体转化细菌; 并在转化细菌中表达该基因。