摘要:
L'invention concerne des enzymes de subtilisine modifiées par mutation d'une séquence de nucléotides (gène) codant pour la subtilisine. Les enzymes de subtilisine modifiées présentent une stabilité thermique augmentée.
摘要:
The present invention provides a composition for cleaning drains clogged with a hair-containing deposit which comprises: (a) a hair-disintegrating amount of a proteolytic enzyme, (b) a disulfide reducing agent, and (c) an alkali metal bisulfite compound in an amount sufficient to enhance the rate of activity of the enzyme, wherein the components (a), (b) and (c) are present as dispersions, separately or in combination, in water-soluble beads, each comprising a water-soluble polymer, such that the enzyme and reducing agent cannot substantially cross-react prior to dissolution of the beads, and wherein the composition on dissolution provides a pH that enhances hair denaturation.
摘要:
A novel enzyme exhibiting proteolytic activity in alkaline media and stability under alkaline conditions is produced by a novel Bacillus strain designated GX6644 or by its mutants or variants. The enzyme is well-suited for inclusion in washing compositions. A culture of GX6644 has been deposited with the American Type Culture Collection, Rockville, Maryland as ATCC No. 53441.
摘要:
Procédé de synthèse de L-tryptophane à partir de glycine, formaldéhyde et indole, utilisant des quantités biocatalytiques de sérine hydroxyméthyltransférase, d'acide tétrahydrofolique et de tryptophane synthétase ou tryptophanase. La sérine hydroxyméthyltransférase et la tryptophane synthétique ou tryptophanase sont ajoutées au milieu de réaction de préférence sous la forme de produits d'expression de microorganismes contenant des vecteurs d'expression codant pour la sérine hydroxyméthyltransférase et la tryptophane synthétase ou tryptophanase.
摘要:
A method of expressing foreign genes in yeast comprising transformation of yeast cells by introduction therein of a cloning vector containing a foreign gene, and yeast galactokinase gene regulatory region and promoter in position to regulate the expression of the foreign gene, and cultivating said transformed yeast cells in a nutrient medium under protein-producing conditions is disclosed. Suitable plasmids and microorganisms useful in the process are also disclosed. The method has been used to produce a GAL 1 - bovine calf prochymosin fusion protein in Saccharom y ces cerevisiae.
摘要:
A method for the enzymatic conversion of t-cinnamic acid and ammonia to L-phenylalanine, using the catalyst, phenylalanine ammonia-lyase, wherein the stability and useful life of the enzyme are improved by conducting the conversion under substantially anaerobic, static conditions.
摘要:
Mutant microbial strains, which are capable of overproducing phenylalanine ammonia-lyase and are useful for the production of L-phenylalanine, may be selected by growing cells on a minimal nutritional medium containing, as substantially the sole carbon source, a phenylalanine analogue, said phenylalanine analogue being a substrate for PAL and the PAL-catalyzed breakdown of said phenylalanine analogue being the rate-determining step for its.overall metabolism.