公开(公告)号：EP0519683A1

公开(公告)日：1992-12-23

申请号：EP92305508.1

申请日：1992-06-16

申请人： GENZYME LIMITED (formerly known as Genzyme (UK) Ltd)

发明人： Jackson, Christopher B. ,  Siddique, Farhat K. ,  Stevens, David R. ,  Moore, Stephen P.

IPC分类号： C07H23/00 ,  C12Q1/40 ,  C07H15/203 ,  C07H3/06

CPC分类号： C07H15/203 ,  C07H17/075 ,  C07H23/00 ,  C12Q1/40 ,  C12Q2334/10

摘要： A process for the direct silylation of the terminal, non-reducing glucose unit of an oligosaccharide containing at least three repeating glucose units and having an aglycone unit at the reducing end thereof, the aglycone being selected from nitrophenyl, 2-chloro-4-nitrophenyl, 4-methylumbeliferone, luciferin and chromogenic phenols, which comprises:

(a) forming a solution of the oligosaccharide in a solvent:
(b) reacting the oligosaccharide with a silylating protective agent in the presence of a base; and
(c) recovering a monosilylated oligosaccharide derivative from the reaction mixture is disclosed.

摘要翻译： 一种用于直接甲硅烷基化终端的方法，含有至少三个重复葡萄糖单元的寡糖的非还原性葡萄糖单元，还原末端具有糖苷配基单元，所述糖苷配基选自硝基苯基，2-氯-4-硝基苯基 包括：（a）在溶剂中形成寡糖的溶液：（b）在碱的存在下使寡糖与甲硅烷基化保护剂反应; 并且（c）从反应混合物中回收单甲硅烷基化的寡糖衍生物。 升