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公开(公告)号：EP0687304B1
公开(公告)日：1997-10-15
申请号：EP94906216.0
申请日：1994-02-03
发明人： BORNKAMM, Georg , POLACK, Axel
CPC分类号： C12N15/85 , C12N15/67 , C12N2710/16222 , C12N2800/108 , C12Q1/6897
摘要： The aim of the invention is to provide a simple quantitative test for the presence of protein kinase C inhibitors. This aim is achieved by means of a DNA construct which contains at least one reporter gene and a control region for the activation of a particularly strongly induced gene of the Epstein-Barr virus (EBV). The protein kinase C inhibitor and a tumour promotor act on EBV-containing cells containing the DNA construct, and the amount of reporter gene product obtained is then determined quantitatively.
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2.发明公开DNA KONSTRUKT UND IN-VITRO-TEST ZUR DETEKTION VON PROTEINKINASE C INHIBITOREN UNTER VERWENDUNG DIESES DNA KONSTRUKTS 失效DNA构建体和体外测试蛋白激酶C抑制剂的检测使用本DNA构建体
公开(公告)号：EP0687304A1
公开(公告)日：1995-12-20
申请号：EP94906216.0
申请日：1994-02-03
发明人： BORNKAMM, Georg , POLACK, Axel
CPC分类号： C12N15/85 , C12N15/67 , C12N2710/16222 , C12N2800/108 , C12Q1/6897
摘要： The aim of the invention is to provide a simple quantitative test for the presence of protein kinase C inhibitors. This aim is achieved by means of a DNA construct which contains at least one reporter gene and a control region for the activation of a particularly strongly induced gene of the Epstein-Barr virus (EBV). The protein kinase C inhibitor and a tumour promotor act on EBV-containing cells containing the DNA construct, and the amount of reporter gene product obtained is then determined quantitatively.
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