公开(公告)号：EP2248896A3

公开(公告)日：2012-07-25

申请号：EP10174533.9

申请日：2004-12-29

申请人： Galapagos N.V.

发明人： Van Rompaey, Luc Juliaan Corina ,  Tomme, Peter Herwig Maria ,  Brown, Robin John

IPC分类号： C12N15/113 ,  A61K31/713 ,  C12Q1/68 ,  C12N5/10 ,  G01N33/68 ,  A61P19/08 ,  A61P19/10

CPC分类号： C12N15/1137 ,  C12N5/0654 ,  C12N2310/11 ,  C12N2310/111 ,  C12N2310/14 ,  C12N2310/315 ,  C12N2310/53 ,  C12N2510/00 ,  C12N2799/021 ,  C12Y207/11001 ,  G01N33/6893

摘要： The present invention provides methods for inducing differentiation of undifferentiated mammalian cells into osteoblasts, and methods for identifying a compound that induces differentiation of undifferentiated mammalian cells into osteoblasts. In addition, the present invention provides polynucleotides and vectors, and the use thereof as a medicament for the treatment of a disease involving a systemic or local decrease in mean bone density. Moreover, the present invention provides methods for in vitro production of bone tissue, osteoblast cells and methods for diagnosing a pathological condition involving a systemic or local decrease in mean bone density or a susceptibility to the condition.

摘要翻译： 本发明提供诱导未分化的哺乳动物细胞分化成成骨细胞的方法，以及鉴定诱导未分化的哺乳动物细胞分化成成骨细胞的化合物的方法。 此外，本发明提供了多核苷酸和载体，以及它们作为用于治疗涉及平均骨密度全身或局部降低的疾病的药物的用途。 此外，本发明提供了用于体外产生骨组织，成骨细胞的方法以及用于诊断涉及平均骨密度或对该病症的易感性的系统或局部降低的病理状态的方法。

公开(公告)号：EP2248897A2

公开(公告)日：2010-11-10

申请号：EP10174536.2

申请日：2004-12-29

申请人： Galapagos N.V.

发明人： Van Rompaye, Luc Juliaan Corina ,  Tomme, Peter Herwig Maria ,  Brown, Robin John

IPC分类号： C12N15/113 ,  A61K31/713 ,  C12Q1/68 ,  C12N5/10 ,  G01N33/68 ,  A61P19/08 ,  A61P19/10

CPC分类号： C12N15/1137 ,  C12N5/0654 ,  C12N2310/11 ,  C12N2310/111 ,  C12N2310/14 ,  C12N2310/315 ,  C12N2310/53 ,  C12N2510/00 ,  C12N2799/021 ,  C12Y207/11001 ,  G01N33/6893

摘要： The present invention provides methods for inducing differentiation of undifferentiated mammalian cells into osteoblasts, and methods for identifying a compound that induces differentiation of undifferentiated mammalian cells into osteoblasts. In addition, the present invention provides polynucleotides and vectors, and the use thereof as a medicament for the treatment of a disease involving a systemic or local decrease in mean bone density. Moreover, the present invention provides methods for in vitro production of bone tissue, osteoblast cells and methods for diagnosing a pathological condition involving a systemic or local decrease in mean bone density or a susceptibility to the condition.

公开(公告)号：EP2214018B1

公开(公告)日：2013-06-26

申请号：EP10158238.5

申请日：2005-04-27

申请人： Galapagos N.V.

发明人： Van Rompaey, Luc Juliaan Corina ,  Tomme, Peter Herwig Maria ,  Brown, Robin John

IPC分类号： C12N15/11 ,  G01N33/566 ,  A61P19/10 ,  G01N33/50

CPC分类号： G01N33/566 ,  C12N15/111 ,  C12N2310/111 ,  C12N2310/14 ,  C12N2310/53 ,  C12N2320/11 ,  C12N2320/12 ,  G01N33/5026 ,  G01N2333/51 ,  G01N2500/04 ,  G01N2800/108

公开(公告)号：EP2214018A3

公开(公告)日：2011-02-09

申请号：EP10158238.5

申请日：2005-04-27

申请人： Galapagos N.V.

发明人： Van Rompaey, Luc Juliaan Corina ,  Tomme, Peter Herwig Maria ,  Brown, Robin John

IPC分类号： G01N33/566 ,  A61P19/10 ,  G01N33/50

CPC分类号： G01N33/566 ,  C12N15/111 ,  C12N2310/111 ,  C12N2310/14 ,  C12N2310/53 ,  C12N2320/11 ,  C12N2320/12 ,  G01N33/5026 ,  G01N2333/51 ,  G01N2500/04 ,  G01N2800/108

摘要： The present invention relates to methods, agents and compound screening assays for inducing differentiation of undifferentiated mammalian cells into osteoblasts. The invention thus provides a method, comprising contacting a compound with a polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID No: 194-309; and measuring a compound-polypeptide property related to the differentiation of said cells. The invention further relates to a bone formation enhancing pharmaceutical composition, and the use thereof in treating and/or preventing a disease involving a systemic or local decrease in mean bone density in a subject. Furthermore, the invention relates to a method for the in vitro production of bone tissue.

摘要翻译： 本发明涉及用于诱导未分化的哺乳动物细胞分化成成骨细胞的方法，试剂和化合物筛选分析。 本发明因此提供了一种方法，包括使化合物与包含选自SEQ ID No：194-309的氨基酸序列的多肽接触; 并测量与所述细胞分化有关的化合物 - 多肽特性。 本发明进一步涉及增强骨形成的药物组合物，及其在治疗和/或预防涉及受试者的平均骨密度全身或局部降低的疾病中的用途。 此外，本发明涉及用于体外生产骨组织的方法。

公开(公告)号：EP2248898A2

公开(公告)日：2010-11-10

申请号：EP10174538.8

申请日：2004-12-29

申请人： Galapagos N.V.

发明人： Van Rompaye, Luc Juliaan Corina ,  Tomme, Peter Herwig Maria ,  Brown, Robin John

IPC分类号： C12N15/113 ,  A61K31/713 ,  C12Q1/68 ,  C12N5/10 ,  A61P19/08 ,  A61P19/10

CPC分类号： C12N15/1137 ,  C12N5/0654 ,  C12N2310/11 ,  C12N2310/111 ,  C12N2310/14 ,  C12N2310/315 ,  C12N2310/53 ,  C12N2510/00 ,  C12N2799/021 ,  C12Y207/11001 ,  G01N33/6893

摘要： The present invention provides methods for inducing differentiation of undifferentiated mammalian cells into osteoblasts, and methods for identifying a compound that induces differentiation of undifferentiated mammalian cells into osteoblasts. In addition, the present invention provides polynucleotides and vectors, and the use thereof as a medicament for the treatment of a disease involving a systemic or local decrease in mean bone density. Moreover, the present invention provides methods for in vitro production of bone tissue, osteoblast cells and methods for diagnosing a pathological condition involving a systemic or local decrease in mean bone density or a susceptibility to the condition.

摘要翻译： 本发明提供诱导未分化哺乳动物细胞分化为成骨细胞的方法，以及用于鉴定诱导未分化哺乳动物细胞分化成成骨细胞的化合物的方法。 此外，本发明提供多核苷酸和载体，以及其用作治疗涉及全身或局部平均骨密度降低的疾病的药物的用途。 此外，本发明提供了用于体外生产骨组织，成骨细胞的方法和用于诊断涉及全身或局部平均骨密度降低或对该病症易感性的病理状态的方法。

公开(公告)号：EP2259063A2

公开(公告)日：2010-12-08

申请号：EP10174527.1

申请日：2005-04-27

申请人： Galapagos N.V.

发明人： Van Rompaye, Luc Juliaan Corina ,  Tomme, Peter Herwig Maria ,  Brown, Robin John

IPC分类号： G01N33/566 ,  A61P19/10 ,  G01N33/50

CPC分类号： G01N33/566 ,  C12N15/111 ,  C12N2310/111 ,  C12N2310/14 ,  C12N2310/53 ,  C12N2320/11 ,  C12N2320/12 ,  G01N33/5026 ,  G01N2333/51 ,  G01N2500/04 ,  G01N2800/108

摘要： The present invention relates to methods, agents and compound screening assays for inducing differentiation of undifferentiated mammalian cells into osteoblasts. The invention thus provides a method, comprising contacting a compound with a polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID No: 194-309; and measuring a compound-polypeptide property related to the differentiation of said cells. The invention further relates to a bone formation enhancing pharmaceutical composition, and the use thereof in treating and/or preventing a disease involving a systemic or local decrease in mean bone density in a subject. Furthermore, the invention relates to a method for the in vitro production of bone tissue.

摘要翻译： 本发明涉及用于诱导未分化哺乳动物细胞分化成成骨细胞的方法，试剂和化合物筛选试验。 因此，本发明提供了一种方法，包括使化合物与包含选自SEQ ID No：194-309的氨基酸序列的多肽接触; 并测量与所述细胞的分化相关的化合物 - 多肽性质。 本发明进一步涉及骨形成增强药物组合物及其在治疗和/或预防涉及受试者平均骨密度全身或局部减少的疾病中的用途。 此外，本发明涉及体外生产骨组织的方法。

公开(公告)号：EP2248896A2

公开(公告)日：2010-11-10

申请号：EP10174533.9

申请日：2004-12-29

申请人： Galapagos N.V.

发明人： Van Rompaye, Luc Juliaan Corina ,  Tomme, Peter Herwig Maria ,  Brown, Robin John

IPC分类号： C12N15/113 ,  A61K31/713 ,  C12Q1/68 ,  C12N5/10 ,  G01N33/68 ,  A61P19/08 ,  A61P19/10

CPC分类号： C12N15/1137 ,  C12N5/0654 ,  C12N2310/11 ,  C12N2310/111 ,  C12N2310/14 ,  C12N2310/315 ,  C12N2310/53 ,  C12N2510/00 ,  C12N2799/021 ,  C12Y207/11001 ,  G01N33/6893

摘要： The present invention provides methods for inducing differentiation of undifferentiated mammalian cells into osteoblasts, and methods for identifying a compound that induces differentiation of undifferentiated mammalian cells into osteoblasts. In addition, the present invention provides polynucleotides and vectors, and the use thereof as a medicament for the treatment of a disease involving a systemic or local decrease in mean bone density. Moreover, the present invention provides methods for in vitro production of bone tissue, osteoblast cells and methods for diagnosing a pathological condition involving a systemic or local decrease in mean bone density or a susceptibility to the condition.

摘要翻译： 本发明提供了用于诱导未分化的哺乳动物细胞的分化成成骨细胞的方法，以及用于鉴定化合物的方法没有诱导未分化的哺乳动物细胞分化成成骨细胞。 另外，本发明提供的多核苷酸和载体，以及作为用于疾病的涉及平均骨密度全身或局部降低的疾病的药物中的用途。 更完了，本发明提供了用于体外骨组织，成骨细胞和用于诊断的病理状态中涉及的平均骨密度全身或局部减小或易感性的条件的方法的生产方法。

公开(公告)号：EP2214018A2

公开(公告)日：2010-08-04

申请号：EP10158238.5

申请日：2005-04-27

申请人： Galapagos N.V.

发明人： Van Rompaey, Luc Juliaan Corina ,  Tomme, Peter Herwig Maria ,  Brown, Robin John

IPC分类号： G01N33/566 ,  A61P19/10 ,  G01N33/50

CPC分类号： G01N33/566 ,  C12N15/111 ,  C12N2310/111 ,  C12N2310/14 ,  C12N2310/53 ,  C12N2320/11 ,  C12N2320/12 ,  G01N33/5026 ,  G01N2333/51 ,  G01N2500/04 ,  G01N2800/108

摘要： The present invention relates to methods, agents and compound screening assays for inducing differentiation of undifferentiated mammalian cells into osteoblasts. The invention thus provides a method, comprising contacting a compound with a polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID No: 194-309; and measuring a compound-polypeptide property related to the differentiation of said cells. The invention further relates to a bone formation enhancing pharmaceutical composition, and the use thereof in treating and/or preventing a disease involving a systemic or local decrease in mean bone density in a subject. Furthermore, the invention relates to a method for the in vitro production of bone tissue.

摘要翻译： 本发明涉及到的方法，试剂和化合物筛选测定法用于诱导未分化的哺乳动物细胞分化成成骨细胞。 因此，本发明提供一种方法，包括与包含选自SEQ ID NO的选择的氨基酸序列的多肽的化合物：194-309; 和测量与细胞分化的化合物的多肽属性说。 本发明还涉及到一个骨形成增强药物组合物，以及治疗和/或预防疾病涉及在受试者中的平均骨密度全身或局部降低其用途。 进一步，本发明涉及一种用于骨组织的体外产生的方法。

公开(公告)号：EP2248898A3

公开(公告)日：2012-07-25

申请号：EP10174538.8

申请日：2004-12-29

申请人： Galapagos N.V.

发明人： Van Rompaey, Luc Juliaan Corina ,  Tomme, Peter Herwig Maria ,  Brown, Robin John

IPC分类号： C12N15/113 ,  A61K31/713 ,  C12Q1/68 ,  C12N5/10 ,  A61P19/08 ,  A61P19/10

CPC分类号： C12N15/1137 ,  C12N5/0654 ,  C12N2310/11 ,  C12N2310/111 ,  C12N2310/14 ,  C12N2310/315 ,  C12N2310/53 ,  C12N2510/00 ,  C12N2799/021 ,  C12Y207/11001 ,  G01N33/6893

摘要： The present invention provides methods for inducing differentiation of undifferentiated mammalian cells into osteoblasts, and methods for identifying a compound that induces differentiation of undifferentiated mammalian cells into osteoblasts. In addition, the present invention provides polynucleotides and vectors, and the use thereof as a medicament for the treatment of a disease involving a systemic or local decrease in mean bone density. Moreover, the present invention provides methods for in vitro production of bone tissue, osteoblast cells and methods for diagnosing a pathological condition involving a systemic or local decrease in mean bone density or a susceptibility to the condition.

公开(公告)号：EP2248897A3

公开(公告)日：2012-07-25

申请号：EP10174536.2

申请日：2004-12-29

申请人： Galapagos N.V.

发明人： Van Rompaey, Luc Juliaan Corina ,  Tomme, Peter Herwig Maria ,  Brown, Robin John

IPC分类号： C12N15/113 ,  A61K31/713 ,  C12Q1/68 ,  C12N5/10 ,  G01N33/68 ,  A61P19/08 ,  A61P19/10

CPC分类号： C12N15/1137 ,  C12N5/0654 ,  C12N2310/11 ,  C12N2310/111 ,  C12N2310/14 ,  C12N2310/315 ,  C12N2310/53 ,  C12N2510/00 ,  C12N2799/021 ,  C12Y207/11001 ,  G01N33/6893

摘要： The present invention provides methods for inducing differentiation of undifferentiated mammalian cells into osteoblasts, and methods for identifying a compound that induces differentiation of undifferentiated mammalian cells into osteoblasts. In addition, the present invention provides polynucleotides and vectors, and the use thereof as a medicament for the treatment of a disease involving a systemic or local decrease in mean bone density. Moreover, the present invention provides methods for in vitro production of bone tissue, osteoblast cells and methods for diagnosing a pathological condition involving a systemic or local decrease in mean bone density or a susceptibility to the condition.