公开(公告)号：EP2400037A1

公开(公告)日：2011-12-28

申请号：EP11006678.4

申请日：2006-10-17

申请人： Gen-Probe Incorporated

发明人： Bungo, Jennifer, J. ,  Hogan, James J. ,  Pollner, Reinhold B. ,  Hudspeth, Marie K. ,  Kaplan, Shanon K. ,  Marlowe, Elizabeth M.

IPC分类号： C12Q1/68

CPC分类号： C12Q1/689 ,  Y02A50/451

摘要： A set of oligonucleotides for use in determining the presence of Legionella pneumophila in a sample, said set of oligonucleotides comprising: a first amplification oligonucleotide, wherein the base sequence of said first amplification oligonucleotide is selected from the group consisting of SEQ ID NO:87, the RNA equivalent of SEQ ID NO:87, and the full complement(s) thereof; a second amplification oligonucleotide, wherein the base sequence of said second amplification oligonucleotide is selected from the group consisting of SEQ ID NO:71, the RNA equivalent of SEQ ID NO:71, and the full complement(s) thereof, and, optionally, a 5' promoter sequence; and a detection probe comprising a target complementary region, wherein the base sequence of said target complementary region is selected from the group consisting of the target complementary region of SEQ ID NO:88, the DNA equivalent of the target complementary region of SEQ ID NO:88, and the full complement(s) thereof.

摘要翻译： 一组用于测定样品中嗜肺军团杆菌存在的寡核苷酸，所述寡核苷酸组包含：第一扩增寡核苷酸，其中所述第一扩增寡核苷酸的碱基序列选自SEQ ID NO：87， SEQ ID NO：87的RNA等效物及其全部补体; 第二扩增寡核苷酸，其中所述第二扩增寡核苷酸的碱基序列选自SEQ ID NO：71，SEQ ID NO：71的RNA当量及其全补体，以及任选地， 5'启动子序列; 和包含靶互补区的检测探针，其中所述靶互补区的碱基序列选自SEQ ID NO：88的靶互补区，SEQ ID NO：88的靶互补区的DNA当量。 88及其全部补充。