公开(公告)号：EP2579897A1

公开(公告)日：2013-04-17

申请号：EP11726020.8

申请日：2011-06-07

申请人： Genentech, Inc.

发明人： BHAKTA, Sunil ,  JUNUTULA, Jagath, R.

IPC分类号： A61K47/48

CPC分类号： C07K16/00 ,  A61K47/48376 ,  A61K47/48569 ,  A61K47/48715 ,  A61K47/6803 ,  A61K47/6811 ,  A61K47/6817 ,  A61K47/6829 ,  A61K47/6855 ,  A61K51/1051 ,  C07K16/3092 ,  C07K16/32 ,  C07K2317/52 ,  C07K2317/55 ,  C07K2319/31

摘要： Cysteine engineered antibodies comprising a free cysteine amino acid in the heavy chain or light chain are prepared by mutagenizing a nucleic acid sequence of a parent antibody and replacing one or more amino acid residues by cysteine to encode the cysteine engineered antibody; expressing the cysteine engineered antibody; and isolating the cysteine engineered antibody. Certain highly reactive cysteine engineered antibodies were identified by the PHESELECTOR assay. Isolated cysteine engineered antibodies may be covalently attached to a capture label, a detection label, a drug moiety, or a solid support.