公开(公告)号：EP3427749A1

公开(公告)日：2019-01-16

申请号：EP18176145.3

申请日：2009-02-19

申请人： GlaxoSmithKline Biologicals SA

发明人： FERNANDEZ, Fabiana ,  WETTER, Michael ,  KOWARIK, Michael ,  WACKER, Michael

IPC分类号： A61K39/02 ,  A61K39/385 ,  A61K39/00 ,  A61K39/108 ,  A61K39/112 ,  A61K39/104 ,  C07K14/195

摘要： The present invention is directed to a bioconjugate vaccine such as an O1-bioconjugate vaccine, comprising: a protein carrier comprising an inserted consensus sequence, D/E - X - N - Z - S/T, wherein X and Z may be any natural amino acid except proline; and at least one antigenic polysaccharide from at least one bacterium, linked to the protein carrier, wherein the at least one antigenic polysaccharide is a bacterial O-antigen from one or more strain of Shigella, E. coli or Pseudomonas aeruginosa; and, optionally, an adjuvant. In another aspect of the present invention is directed to a method of producing a O1- bioconjugate in a bioreactor comprising a number of steps.

公开(公告)号：EP3527663A1

公开(公告)日：2019-08-21

申请号：EP19150645.0

申请日：2013-10-11

申请人： GlaxoSmithKline Biologicals SA

发明人： WACKER, Michael ,  KOWARIK, Michael ,  FERNANDEZ, Fabiana

IPC分类号： C12N15/90 ,  C12N9/10 ,  C12N15/52 ,  C12N15/64 ,  C12P19/28

摘要： Described herein are novel methods of inserting nucleic acid sequences into host cells. Also described herein are genetically stable host cells comprising inserted nucleic acid sequences and methods of using such host cells in the generation of proteins.