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公开(公告)号:EP2276852A2
公开(公告)日:2011-01-26
申请号:EP09747459.7
申请日:2009-05-13
发明人: SCHOFIELD, David, A.
IPC分类号: C12Q1/02
CPC分类号: C12Q1/04 , C12Q1/6888 , C12Q2600/158 , G01N2333/24
摘要: The present disclosure relates to compositions, methods, systems and kits for the detection of microorganisms of the Yersinia species including Yersinia pestis. The disclosure relates to recombinant phage operable to infect a Yersinia microorganism, the phage comprising a detectable reporter. Detection systems of the disclosure may comprise a phage operable to infect a Yersinia microorganism, and may comprise a reporter nucleic acid expressible upon infection of a Yersinia microorganism by the phage. The system may be operable to detect the expression of the reporter. A detectable reporter may comprise any gene having bioluminescent, colorimetric and/or visual detectability. For example, a detectable reporter may comprise one or more luxAB genes detectable by emission, enhancement and/or change in spectrum of bioluminescent light. Live and infectious Yersinia microbes may be detected by the compositions, methods, systems and kits described herein.
摘要翻译: 本公开涉及用于检测包括鼠疫耶尔森氏菌在内的耶尔森氏菌属物种的微生物的组合物,方法,系统和试剂盒。 本公开涉及可操作以感染耶尔森菌属微生物的重组噬菌体,所述噬菌体包含可检测的报道分子。 本公开的检测系统可包含可操作以感染耶尔森菌属微生物的噬菌体,并且可包含通过噬菌体感染耶尔森氏菌属微生物可表达的报道核酸。 该系统可用于检测报道分子的表达。 可检测报道分子可以包含具有生物发光,比色和/或视觉可检测性的任何基因。 例如,可检测的报道分子可以包含一种或多种可通过生物发光光谱的发射,增强和/或变化检测到的luxAB基因。 活的和传染性耶尔森氏菌微生物可以通过本文描述的组合物,方法,系统和试剂盒检测。
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公开(公告)号:EP2276852B1
公开(公告)日:2015-11-04
申请号:EP09747459.7
申请日:2009-05-13
IPC分类号: C12Q1/02
CPC分类号: C12Q1/04 , C12Q1/6888 , C12Q2600/158 , G01N2333/24
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