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1.发明公开
公开(公告)号：EP3562953A1
公开(公告)日：2019-11-06
申请号：EP18710168.8
申请日：2018-01-02
IPC分类号： C12Q1/04 , G01N33/569
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公开(公告)号：EP2276852A2
公开(公告)日：2011-01-26
申请号：EP09747459.7
申请日：2009-05-13
发明人： SCHOFIELD, David, A.
IPC分类号： C12Q1/02
CPC分类号： C12Q1/04 , C12Q1/6888 , C12Q2600/158 , G01N2333/24
摘要： The present disclosure relates to compositions, methods, systems and kits for the detection of microorganisms of the Yersinia species including Yersinia pestis. The disclosure relates to recombinant phage operable to infect a Yersinia microorganism, the phage comprising a detectable reporter. Detection systems of the disclosure may comprise a phage operable to infect a Yersinia microorganism, and may comprise a reporter nucleic acid expressible upon infection of a Yersinia microorganism by the phage. The system may be operable to detect the expression of the reporter. A detectable reporter may comprise any gene having bioluminescent, colorimetric and/or visual detectability. For example, a detectable reporter may comprise one or more luxAB genes detectable by emission, enhancement and/or change in spectrum of bioluminescent light. Live and infectious Yersinia microbes may be detected by the compositions, methods, systems and kits described herein.
摘要翻译： 本公开涉及用于检测包括鼠疫耶尔森氏菌在内的耶尔森氏菌属物种的微生物的组合物，方法，系统和试剂盒。 本公开涉及可操作以感染耶尔森菌属微生物的重组噬菌体，所述噬菌体包含可检测的报道分子。 本公开的检测系统可包含可操作以感染耶尔森菌属微生物的噬菌体，并且可包含通过噬菌体感染耶尔森氏菌属微生物可表达的报道核酸。 该系统可用于检测报道分子的表达。 可检测报道分子可以包含具有生物发光，比色和/或视觉可检测性的任何基因。 例如，可检测的报道分子可以包含一种或多种可通过生物发光光谱的发射，增强和/或变化检测到的luxAB基因。 活的和传染性耶尔森氏菌微生物可以通过本文描述的组合物，方法，系统和试剂盒检测。
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公开(公告)号：EP1885853A2
公开(公告)日：2008-02-13
申请号：EP06844110.4
申请日：2006-05-26
IPC分类号： C12N15/10 , G01N33/50 , G01N33/543 , C12Q1/68
CPC分类号： C12N15/1037 , G01N33/554
摘要： The present disclosure provides a biological detection system and method. One detection system may include a genetically engineered phage that expresses a surface molecule able to bind a target molecule; a bacterium susceptible to infection by the phage; and a detection component able to determine whether the bacterium has been infected by the phage. Infection of a bacterium by a phage may be indicative of phage binding to the target molecule. One method may include placing a sample suspected of containing the target molecule with a binder; adding a genetically engineered phage having reporter genetic material and able to bind the target molecule; washing away unbound phage; releasing phage bound to the target molecule; infecting a bacterium with the released phage; and detecting the presence of any reporter genetic material in the bacterium. Reporter material in the bacterium may correlate with target molecule in the sample.
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4.发明公开
公开(公告)号：EP3692160A1
公开(公告)日：2020-08-12
申请号：EP18792716.5
申请日：2018-10-01
IPC分类号： C12Q1/04 , C12Q1/18 , C12Q1/34 , C12N7/00 , C12Q1/6869
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公开(公告)号：EP2140018B1
公开(公告)日：2012-08-01
申请号：EP08826012.0
申请日：2008-04-04
IPC分类号： C12Q1/04 , C12Q1/66 , G01N33/569 , C12Q1/70
CPC分类号： C12Q1/04 , C12N15/1086 , C12N2795/00011 , C12N2795/10311 , C12N2795/14111 , C12Q1/66 , G01N33/569 , G01N33/56983 , G01N2333/32
摘要： The present disclosure, according to some embodiments, relates to phage-based biological detection systems, compositions, and methods. In some embodiments, it relates to a detection system and method using phage binding and bacterial infection to detect the presence of a target molecule (e.g., a toxin). One detection system may include a genetically engineered phage that expresses a surface molecule able to bind a target molecule and/or target microorganism; a bacterium susceptible to infection by the phage; and a detection component able to determine whether the bacterium has been infected by the phage. Infection of a bacterium by a phage may be indicative of phage binding to the target molecule and/or target microorganism. One method may include placing a sample suspected of containing the target molecule and/or target microorganism with a binder; adding a genetically engineered phage having reporter genetic material and able to bind the target molecule and/or target microorganism; washing away unbound phage; releasing phage bound to the target molecule and/or target microorganism; infecting a bacterium with the released phage; and detecting the presence of any reporter genetic material in the bacterium. Reporter material in the bacterium may correlate with target molecule and/or target microorganism in the sample. In some embodiments, the disclosure relates to a detection system and method using phage comprising a reporter to infect a microorganism (e.g., Bacillus anthracis), wherein the reporter is selectively (e.g., only) detectable upon phage infection.
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6.发明公开DIFFERENTIALLY FLUORESCENT YEAST BIOSENSORS FOR THE DETECTION AND BIODEGRADATION OF CHEMICAL AGENTS 审中-公开差异荧光HEFEBIOSENSOREN识别和化学试剂的生物去除
公开(公告)号：EP2195427A2
公开(公告)日：2010-06-16
申请号：EP08863866.3
申请日：2008-09-30
IPC分类号： C12N15/09
CPC分类号： C12Q1/025 , G01N33/84 , G01N2333/39
摘要： The present disclosure provides methods, devices, systems and compositions for detecting and/or modifying chemical agents. In some embodiments, a biosensor may be configured to detect a chemical agent, modify that agent to a form with reduced toxicity, and and/or detect the modified form of the chemical agent. The present disclosure also relates, in some embodiments, to variant organophosphorus hydrolase having one or more desirable amino acid substitutions.
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公开(公告)号：EP0904158B1
公开(公告)日：2002-07-24
申请号：EP96910384.5
申请日：1996-03-06
CPC分类号： B29C41/36 , B29C64/112 , B29K2105/0002 , B29K2105/0073
摘要： A preparation of a self-supporting or free-standing three-dimensional unitary structural body (32) by a method including generating successively a plurality of cross-sectional layers of the body, one layer on top of the other and with the layers joined together to form the body and with the generating of a cross-sectional layer comprising placing a plurality of drops (27) of a liquid composition containing a first reactant in a pattern of discrete drops making up the cross-sectional layer (25). A plurality of discrete drops of another liquid composition containing another reactant is subsequently applied in contact with the placed drops of the pattern so that the first reactant and the other reactant react to form a solid.
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公开(公告)号：EP3473716A1
公开(公告)日：2019-04-24
申请号：EP18201369.8
申请日：2018-10-18
摘要： Reactive and sorbent materials including a non-encapsulated crosslinked biological material immobilized on a support matrix that includes a polyamine and at least one support material are described. The support material can be an inorganic or organic support material. The reactive and sorbent materials are formed by reacting the biological material with the polyamine, at least one support material, and a crosslinking agent. The materials exhibit enhanced properties generally, are capable of maintaining their reactive and sorbent properties in contact with digestive fluids, and exhibiting their reactive and sorbent properties as they pass throughout an organism's entire digestive system. Reactive and sorbent materials in contact with digestive juices at pH's ranging from about 3 to about 7 have maintained their reactive and sorbent properties for at least 4 hours.
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公开(公告)号：EP2102346A2
公开(公告)日：2009-09-23
申请号：EP08795774.2
申请日：2008-01-07
IPC分类号： C12N15/55 , C12N9/14 , C12N9/24 , C12N9/50 , C12N9/80 , C12N1/19 , C12N1/36 , A61K38/46 , A61L2/00
CPC分类号： C12N9/14 , A61K38/00 , A61L2/18 , A62D3/02 , A62D2101/02 , C12N1/18 , C12N1/36 , C12N9/2402 , C12N9/503 , C12N9/80
摘要： The present disclosure relates to yeast biocatalysts and methods of using yeast biocatalysts for detoxifying a biowarfare agent. In some embodiments, a yeast biocatalyst may include a nucleic acid encoding a phage lysin operably linked to a prepro leader sequence, an expression control sequence operably linked to the nucleic acid, and a bioeffective amount (e.g., enough to detoxify) of the phage lysin. A yeast biocatalyst may include an engineered strain of a Saccharomyces cerevisiae. A phage lysin may be selected from the group consisting of PlyG and PlyPH. A method of detoxifying a biowarfare agent may include contacting a biowarfare agent with a yeast biocatalyst under conditions that permit detoxification. Conditions that permit detoxification, according to some embodiments, may include conditions that permit expression and secretion of a phage lysin. In some embodiments, conditions that permit detoxification may include conditions that permit germination of a spore. For example, a method of detoxifying a biowarfare agent spore may include contacting the spore with growth media (e.g., complete media or complex media) having alanine.
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10.发明公开DIFFERENTIALLY FLUORESCENT YEAST BIOSENSORS FOR THE DETECTION AND BIODEGRADATION OF CHEMICAL AGENTS 审中-公开差异荧光HEFEBIOSENSOREN识别和化学试剂的生物去除
公开(公告)号：EP1963538A2
公开(公告)日：2008-09-03
申请号：EP06851299.5
申请日：2006-11-29
IPC分类号： C12Q1/68
CPC分类号： C12Q1/025
摘要： The present disclosure provides methods, devices, systems and compositions for detecting and/or modifying chemical agents. In some embodiments, a biosensor may be configured to detect a chemical agent, modify that agent to a form with reduced toxicity, and and/or detect the modified form of the chemical agent.
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