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1.发明授权REAGENTS AND METHODS FOR DETECTING INFLUENZA VIRUS PROTEINS 有权VENFAHREN ZUM NACHWEIS VEN INFLUENZAVIRUSPROTEINEN的REAGENZIEN
公开(公告)号：EP2437779B1
公开(公告)日：2016-10-26
申请号：EP10782851.9
申请日：2010-05-28
发明人： LI, Xuguang , HE, Runtao , VAN DOMSELAAR , Gary
IPC分类号： A61K39/00 , A61K39/12 , A61K39/145 , G01N33/573 , C07K16/10 , C12N9/24 , G01N33/569 , C07K16/40
CPC分类号： G01N33/56983 , A61K39/12 , A61K39/145 , A61K2039/505 , A61K2039/55566 , A61K2039/6081 , C07K16/1018 , C07K16/40 , C07K2317/34 , C12N9/2402 , C12N2760/16134 , C12Y302/01018 , G01N33/573 , G01N2333/924 , G01N2333/9506
摘要： Two universally conserved sequences from influenza type A neuraminidases were identified by large scale sequence analysis then chemically modified and conjugated to carrier proteins to generate mono-specific and monoclonal antibodies. The two antibodies, one targeting the N-terminus of the type A neuraminidase and the other sequence close to enzymatic active site, were capable of binding to all 9 subtypes of neuraminidase while demonstrating remarkable specificity against the viral neuraminidase sequences since no cross-reactivity against allantoic proteins was observed. Quantitative analyses of NA using slot blot suggest that the antibodies can be used for NA antigen quantitation in vaccines. These represent the first time the antibody-based immunoassay can be used for NA quantitative determination.
摘要翻译： 通过大规模序列分析鉴定了来自甲型流感A型神经氨酸酶的两种普遍保守的序列，然后化学修饰并与载体蛋白缀合以产生单特异性和单克隆抗体。 靶向A型神经氨酸酶的N末端的两种抗体和接近酶活性位点的其他序列能够结合神经氨酸酶的所有9种亚型，同时显示出对病毒神经氨酸酶序列的显着特异性，因为没有交叉反应 观察尿囊蛋白。 使用狭缝印迹的NA的定量分析表明该抗体可用于疫苗中的NA抗原定量。 这些代表了首次基于抗体的免疫测定可用于NA定量测定。
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公开(公告)号：EP2437779A1
公开(公告)日：2012-04-11
申请号：EP10782851.9
申请日：2010-05-28
发明人： LI, Xuguang , HE, Runtao , VAN DOMSELAAR , Gary
IPC分类号： A61K39/385 , A61K39/145 , A61P31/16 , A61P37/04 , C07K16/10 , C07K16/40 , C12N11/02 , C12N9/24 , C12P21/08 , G01N33/15 , G01N33/573
CPC分类号： G01N33/56983 , A61K39/12 , A61K39/145 , A61K2039/505 , A61K2039/55566 , A61K2039/6081 , C07K16/1018 , C07K16/40 , C07K2317/34 , C12N9/2402 , C12N2760/16134 , C12Y302/01018 , G01N33/573 , G01N2333/924 , G01N2333/9506
摘要： Two universally conserved sequences from influenza type A neuraminidases were identified by large scale sequence analysis then chemically modified and conjugated to carrier proteins to generate mono-specific and monoclonal antibodies. The two antibodies, one targeting the N-terminus of the type A neuraminidase and the other sequence close to enzymatic active site, were capable of binding to all 9 subtypes of neuraminidase while demonstrating remarkable specificity against the viral neuraminidase sequences since no cross-reactivity against allantoic proteins was observed. Quantitative analyses of NA using slot blot suggest that the antibodies can be used for NA antigen quantitation in vaccines. These represent the first time the antibody-based immunoassay can be used for NA quantitative determination.
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