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公开(公告)号：EP2437779A1
公开(公告)日：2012-04-11
申请号：EP10782851.9
申请日：2010-05-28
发明人： LI, Xuguang , HE, Runtao , VAN DOMSELAAR , Gary
IPC分类号： A61K39/385 , A61K39/145 , A61P31/16 , A61P37/04 , C07K16/10 , C07K16/40 , C12N11/02 , C12N9/24 , C12P21/08 , G01N33/15 , G01N33/573
CPC分类号： G01N33/56983 , A61K39/12 , A61K39/145 , A61K2039/505 , A61K2039/55566 , A61K2039/6081 , C07K16/1018 , C07K16/40 , C07K2317/34 , C12N9/2402 , C12N2760/16134 , C12Y302/01018 , G01N33/573 , G01N2333/924 , G01N2333/9506
摘要： Two universally conserved sequences from influenza type A neuraminidases were identified by large scale sequence analysis then chemically modified and conjugated to carrier proteins to generate mono-specific and monoclonal antibodies. The two antibodies, one targeting the N-terminus of the type A neuraminidase and the other sequence close to enzymatic active site, were capable of binding to all 9 subtypes of neuraminidase while demonstrating remarkable specificity against the viral neuraminidase sequences since no cross-reactivity against allantoic proteins was observed. Quantitative analyses of NA using slot blot suggest that the antibodies can be used for NA antigen quantitation in vaccines. These represent the first time the antibody-based immunoassay can be used for NA quantitative determination.
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2.发明授权REAGENTS AND METHODS FOR DETECTING INFLUENZA VIRUS PROTEINS 有权VENFAHREN ZUM NACHWEIS VEN INFLUENZAVIRUSPROTEINEN的REAGENZIEN
公开(公告)号：EP2437779B1
公开(公告)日：2016-10-26
申请号：EP10782851.9
申请日：2010-05-28
发明人： LI, Xuguang , HE, Runtao , VAN DOMSELAAR , Gary
IPC分类号： A61K39/00 , A61K39/12 , A61K39/145 , G01N33/573 , C07K16/10 , C12N9/24 , G01N33/569 , C07K16/40
CPC分类号： G01N33/56983 , A61K39/12 , A61K39/145 , A61K2039/505 , A61K2039/55566 , A61K2039/6081 , C07K16/1018 , C07K16/40 , C07K2317/34 , C12N9/2402 , C12N2760/16134 , C12Y302/01018 , G01N33/573 , G01N2333/924 , G01N2333/9506
摘要： Two universally conserved sequences from influenza type A neuraminidases were identified by large scale sequence analysis then chemically modified and conjugated to carrier proteins to generate mono-specific and monoclonal antibodies. The two antibodies, one targeting the N-terminus of the type A neuraminidase and the other sequence close to enzymatic active site, were capable of binding to all 9 subtypes of neuraminidase while demonstrating remarkable specificity against the viral neuraminidase sequences since no cross-reactivity against allantoic proteins was observed. Quantitative analyses of NA using slot blot suggest that the antibodies can be used for NA antigen quantitation in vaccines. These represent the first time the antibody-based immunoassay can be used for NA quantitative determination.
摘要翻译： 通过大规模序列分析鉴定了来自甲型流感A型神经氨酸酶的两种普遍保守的序列，然后化学修饰并与载体蛋白缀合以产生单特异性和单克隆抗体。 靶向A型神经氨酸酶的N末端的两种抗体和接近酶活性位点的其他序列能够结合神经氨酸酶的所有9种亚型，同时显示出对病毒神经氨酸酶序列的显着特异性，因为没有交叉反应 观察尿囊蛋白。 使用狭缝印迹的NA的定量分析表明该抗体可用于疫苗中的NA抗原定量。 这些代表了首次基于抗体的免疫测定可用于NA定量测定。
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公开(公告)号：EP2265646B1
公开(公告)日：2016-01-27
申请号：EP09721145.2
申请日：2009-03-10
IPC分类号： C07K19/00 , A61K39/145 , A61K39/385 , C07K14/11 , C07K16/10 , G01N33/15 , G01N33/543 , G01N33/569
CPC分类号： C07K16/1018 , C07K14/005 , C12N2760/16122 , G01N2333/11
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公开(公告)号：EP2424879A1
公开(公告)日：2012-03-07
申请号：EP10769178.4
申请日：2010-04-29
发明人： HE, Runtao , LI, Xuguang , VAN DOMSELAAR, Gary
IPC分类号： C07K14/11 , A61K39/145 , A61K39/385 , A61P31/16 , C07K7/08
CPC分类号： A61K39/145 , A61K39/00 , A61K39/12 , A61K2039/505 , A61K2039/55566 , A61K2039/6081 , C07K14/005 , C07K16/1018 , C07K2317/76 , C12N2760/16122 , C12N2760/16134 , C12N2760/16222 , C12N2760/16234
摘要： The present invention discloses isolated peptides encoding an antigen or fragments thereof from the N-terminus of hemagglutinin protein of influenza, methods for isolating such antigens and specific uses thereof. The peptide can be used as a vaccine to generate an antibody response that neutralizes influenza infectivtty against a variety of influenza strains.
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公开(公告)号：EP2618825A1
公开(公告)日：2013-07-31
申请号：EP11827867.0
申请日：2011-09-16
发明人： MYSKIW, Chad , CAO, Jingxin , HE, Runtao
CPC分类号： A61K31/35
摘要： Poxviruses remain a significant public health concern due to their potential use as bioterrorist agents and the spread of animal borne poxviruses, such as monkeypox virus, to humans. Thus, the identification of small molecule inhibitors of poxvirus replication is warranted. Vaccinia virus is the prototypic member of the
Orthopoxvirus genus, which also includes variola and monkeypox virus. In this study, we demonstrate that the carboxylic ionophore nigericin is a potent inhibitor of vaccinia virus replication in several human cell lines, in HeLa cells, we found that the 50% inhibitory concentration of nigericin against vaccinia virus was 7.9 nM, with a selectivity index of 1038. We present data demonstrating that nigericin targets vaccinia virus replication at a post-entry stage. While nigericin moderately inhibits both early vaccinia gene transcription and translation, viral DNA replication and intermediate and late gene expression are severely compromised in the presence of nigericin. Our results demonstrate that nigericin has the potential to be further developed into an effective antiviral to treat poxvirus infections.-
公开(公告)号：EP2265646A1
公开(公告)日：2010-12-29
申请号：EP09721145.2
申请日：2009-03-10
IPC分类号： C07K19/00 , A61K39/145 , A61K39/385 , C07K14/11 , C07K16/10 , G01N33/15 , G01N33/543 , G01N33/569
CPC分类号： C07K16/1018 , C07K14/005 , C12N2760/16122 , G01N2333/11
摘要： Antibodies that specifically bind to a peptide having an amino acid sequence as found at the N-terminus of the HA2 fusion peptide of the influenza A virus may be raised by inoculating a mammal with a conjugate of the peptide. In one embodiment, the conjugate comprises the peptide linked to a spacer (e.g. 6-aminocaproic acid) and a carrier protein (e.g. KLH). The antibodies may be used as a universal reagent for detecting HA proteins of influenza viruses. The antibodies are useful as versatile reagents for laboratory research and vaccine potency determination, especially in the event of pandemic influenza outbreaks.
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