公开(公告)号：EP1072688B1

公开(公告)日：2004-06-30

申请号：EP98914021.5

申请日：1998-04-15

申请人： Hidaka, Hiroyoshi

发明人： HIDAKA, Hiroyoshi ,  TANAKA, Hideki

IPC分类号： C12N15/10

CPC分类号： C12N15/1048

摘要： A method for in vivo detecting a target protein gene by a drug, which comprises binding an antigenic substance to a drug to be administered to a living organism via a chemical crosslinker, using the obtained material as a probe, and directly screening the gene of the protein bonded to the above probe by using a cDNA expression library containing genes of the living organism to which the drug is to be administered. Compared with the conventional drug-fixed column methods, the above method makes it possible to directly and conveniently isolate the gene of the target molecule without resort to the purification of the protein and the analysis of the amino acid sequence thereof.

公开(公告)号：EP1072688A1

公开(公告)日：2001-01-31

申请号：EP98914021.5

申请日：1998-04-15

申请人： Hidaka, Hiroyoshi

发明人： HIDAKA, Hiroyoshi ,  TANAKA, Hideki

IPC分类号： C12Q1/68 ,  G01N33/53

CPC分类号： C12N15/1048

摘要： A method for detecting a gene of a drug-targeted protein in a living organism, which method includes causing an antigenic substance to be bound to a drug via a chemical cross-linker, the drug being administered to the living organism; using the obtained material as a probe; and directly screening the gene of the protein bound to the probe by use of a cDNA expression library containing genes of the living organism to which the drug is to be administered.
The method of the present invention has eliminated the need for the protein purification step and amino acid sequence analysis, which are necessary when a conventional drug-fixed column method is performed. Also, the method of the present invention has enabled direct and simple isolation of the gene of the protein to which the drug is targeted.

摘要翻译： 一种用于检测活体中药物靶向蛋白质的基因的方法，该方法包括使抗原物质通过化学交联剂与药物结合，所述药物被施用于所述活体; 使用获得的材料作为探针; 并通过使用含有待给药药物的生物体的基因的cDNA表达文库直接筛选与探针结合的蛋白质的基因。 本发明的方法消除了在进行常规药物固定柱方法时必需的蛋白质纯化步骤和氨基酸序列分析的需要。 此外，本发明的方法能够直接和简单地分离药物靶向的蛋白质的基因。