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    SINGLE MOLECULE REAL TIME SEQUENCER, NUCLEIC ACID ANALYZER AND SINGLE MOLECULE REAL TIME SEQUENCING METHOD
    1.
    发明公开
    SINGLE MOLECULE REAL TIME SEQUENCER, NUCLEIC ACID ANALYZER AND SINGLE MOLECULE REAL TIME SEQUENCING METHOD 有权
    单分子实时测序仪，核酸分析仪和单分子实时测序方法

    公开(公告)号：EP2292727A1

    公开(公告)日：2011-03-09

    申请号：EP09770037.1

    申请日：2009-06-08

    申请人： Hitachi High-Technologies Corporation

    发明人： KATO, Hirokazu ,  TAKAHASHI, Satoshi ,  KUMAZAKI, Nobutaka ,  HAGA, Takanobu

    IPC分类号： C12M1/00 ,  C12Q1/68 ,  G01N21/78 ,  G01N37/00

    CPC分类号： C12Q1/6874 ,  B01J2219/00529 ,  B01J2219/00596 ,  B01J2219/00608 ,  B01J2219/00711 ,  B01J2219/00722 ,  B01L3/502707 ,  B82Y30/00 ,  G01N21/648 ,  C12Q2523/313 ,  C12Q2523/319 ,  C12Q2561/113 ,  C12Q2565/628 ,  C12Q2525/186 ,  C12Q2563/107 ,  C12Q2565/631

    摘要： An object of the present invention relates to selectively control an extension reaction within a desired area in a substrate. In the present invention, an oligo probe arranged with a caged compound at the terminal thereof is immobilized to a reaction field area in the substrate. After pouring a reaction solution into a flow cell including the reaction field area, the reaction field area alone is irradiated with light to associate the photodegradation-active protecting group at the terminal of the oligo probe that has been immobilized in the reaction field area and thus to selectively control initiation of a polymerase extension reaction. In the flow cell, a plural number of the reaction field areas are arranged at constant interval on the substrate. The flow cell immobilized to a moving stage is moved by a distance equal to the interval between the adjacent reaction field areas and then light irradiation is carried out to measure the extension reaction continuously. By repeating these operations, the base extension reaction is stably measured in the flow cell without using complicated channels or without replacing the reaction solution.

    摘要翻译： 本发明的一个目的涉及选择性地控制衬底中期望区域内的延伸反应。 在本发明中，将在其末端布置有笼状化合物的寡核苷酸探针固定到基底中的反应场区域。 在将反应溶液注入到包含反应场区域的流通池中之后，单独对反应场区域照射光以使已固定在反应场区域中的寡聚物探针的末端处的光降解活性保护基团缔合 以选择性地控制聚合酶延伸反应的启动。 在流通池中，多个反应场区以恒定的间隔排列在衬底上。 固定在移动台上的流动池移动与相邻反应场区之间的间隔相等的距离，然后进行光照以连续测量延伸反应。 通过重复这些操作，在流通池中稳定地测量碱扩展反应而不使用复杂的通道或不更换反应溶液。