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1.发明公开METHOD FOR ANALYZING BIOMOLECULES AND BIOMOLECULE ANALYZER 审中-公开VERFAHREN ZUR ANALYZE VONBIOMOLEKÜLENUNDBIOMOLEKÜLANALYSATOR
公开(公告)号：EP2765424A1
公开(公告)日：2014-08-13
申请号：EP12838806.3
申请日：2012-10-04
发明人： SAITO, Toshiro , HAMASAKI, Koshin , TAKAHASHI, Satoshi , MAESHIMA, Muneo , IMAI, Kyoko , IMAI, Kazumichi , TAO, Ryuji
IPC分类号： G01N33/553 , C12M1/00 , C12Q1/68 , G01N21/64 , G01N33/543
CPC分类号： C12Q1/6834 , C12Q1/6804 , C12Q1/6837 , G01N21/6428 , G01N21/6452 , G01N33/54326 , G01N33/54346 , G01N33/54393 , G01N33/587 , G01N2021/6421 , C12Q2525/161 , C12Q2545/114 , C12Q2563/107 , C12Q2563/143 , C12Q2563/149 , C12Q2565/514 , C12Q2563/119
摘要： An object of the present invention is to provide a method and means for analyzing biomolecules that can realize, in biomolecule analysis, a wide dynamic range attained by counting the number of biomolecules and rapid analysis. The present invention relates to a method for analyzing biomolecules, comprising the steps of: immobilizing biomolecules 101 to be analyzed on surfaces of magnetic microparticles 108; reacting labeled probe molecules 104 with the biomolecules 101 to be analyzed; collecting and immobilizing the microparticles 108 on a support substrate 110; and measuring a label on the support substrate 110. Since single-molecule immobilized magnetic microparticles are used in the present invention, the number of biomolecules can be counted, and since hybridization and an antigen-antibody reaction are performed with the microparticles having biomolecules immobilized thereon dispersed, the reaction can be rapidly performed. Further, the type and the abundance of biomolecules of interest can be determined at a single molecular level, so as to evaluate, in particular, the absolute concentration of biomolecules.
摘要翻译： 本发明的目的是提供一种用于分析生物分子的方法和装置，其可以在生物分子分析中实现通过计数生物分子数量和快速分析获得的宽动态范围。 本发明涉及一种分析生物分子的方法，其特征在于包括以下步骤：将待分析的生物分子101固定在磁性微粒108的表面上; 使标记的探针分子104与待分析的生物分子101反应; 将微粒108收集并固定在支撑基板110上; 并测量支撑基板110上的标签。由于在本发明中使用单分子固定的磁性微粒，因此可以计数生物分子的数量，并且由于在其上固定有生物分子的微粒进行杂交和抗原 - 抗体反应 分散，可迅速进行反应。 此外，感兴趣的生物分子的类型和丰度可以在单个分子水平上确定，以便特别地评估生物分子的绝对浓度。
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2.发明授权
公开(公告)号：EP2292727B1
公开(公告)日：2013-10-23
申请号：EP09770037.1
申请日：2009-06-08
CPC分类号： C12Q1/6874 , B01J2219/00529 , B01J2219/00596 , B01J2219/00608 , B01J2219/00711 , B01J2219/00722 , B01L3/502707 , B82Y30/00 , G01N21/648 , C12Q2523/313 , C12Q2523/319 , C12Q2561/113 , C12Q2565/628 , C12Q2525/186 , C12Q2563/107 , C12Q2565/631
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3.发明公开
公开(公告)号：EP2500424A1
公开(公告)日：2012-09-19
申请号：EP10829686.4
申请日：2010-11-04
摘要： When cells are analyzed, fractionated, and incubated while keeping the cells alive, real-time operations can be performed more easily and the cells can be incubated while removing unnecessary cells from the incubated cells to purify the cells being incubated. Furthermore, desired cells are separated through analysis from the incubated cells, and the purity, recovery, and viability of the cells are heightened. Use is made of a substrate having photo-controllable cell adhesion properties, the substrate comprising a transparent base and, formed thereon, a film of a material which has photo-controllable cell adhesion properties and has been obtained by bonding a cell-adhesive material to a cell-non-adhesive material through photo-dissociable groups. Cell images are detected and analyzed to obtain information about the location of desired cells. On the basis of the information, a space is formed between cells and the material having photo-controllable cell adhesion properties is cut, by means of second light irradiation. Meanwhile, by means of first light irradiation, the surface of the substrate is changed from a cell-adhesive surface to a cell-non-adhesive surface, thereby separating the cell(s) from the substrate. Thus, cells can be analyzed and fractionated while keeping the cells alive.
摘要翻译： 当细胞被分析，分级，并温育，同时保持细胞存活，实时操作可以更容易地进行和细胞可以同时去除从所述温育的细胞的细胞不必要以纯化被孵育的细胞一起温育。 进一步，希望的细胞通过分析从培养的细胞中分离，纯度，回收率，并且细胞的存活率提高。 使用具有光可控的细胞粘附性的底物，所述底物包括一个透明基体和在其上形成，其具有光控制细胞粘附属性，并通过一个细胞粘附性材料结合到已获得的材料制成的膜 通过光解离性基团的细胞非粘着材料。 被检测和分析，以获得关于期望的细胞的位置的信息单元的图像。 上的信息的基础上，一个空间被细胞和具有光可控的细胞粘附特性的材料被切断，由第2光照射的装置之间形成。 同时，由第1光照射手段，基板的表面被从一个细胞粘附性表面改变为细胞非粘性表面，从而分离从基片上的细胞（或多个）。 因此，细胞可以分析和分级，同时保持细胞存活。
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4.发明公开NUCLEIC ACID ANALYSIS METHOD AND ANALYSIS APPARATUS 有权NUKLEINSÄURE-ANALYSEVERFAHREN UND-ANALYSEVORRICHTUNG
公开(公告)号：EP2735618A1
公开(公告)日：2014-05-28
申请号：EP12815243.6
申请日：2012-05-16
发明人： SAITO, Toshiro , HAMASAKI, Koshin , TAKAHASHI, Satoshi , MAESHIMA, Muneo , IMAI, Kyoko , IMAI, Kazumichi , TAO, Ryuji
CPC分类号： C12Q1/6834 , C12N15/1065 , C12Q2525/207 , C12Q2537/125 , C12Q2537/143 , C12Q2563/143 , C12Q2563/149
摘要： The object of the present invention is to provide a convenient method for nucleic acid analysis, which enables 1000 or more types of nucleic acid to be analyzed collectively with high comprehensiveness and with a dynamic range of at least four digits. In particular, provide is a very effective analytical method especially for untranslated RNAs and microRNAs, of which the types of target nucleic acids is 10000 or lower.
The present invention enables nucleic acids to be analyzed conveniently and rapidly with high comprehensiveness and quantitative performance at single-molecule sensitivity and resolution by following the steps of: preparing a group of target nucleic acid fragments one molecule at a time and hybridizing the nucleic acid molecules, which have known base sequences and have been labeled with the fluorescence substances, with the group of the target nucleic acid fragments to detect the fluorescence substances labeling the hybridized nucleic acid molecules.摘要翻译： 本发明的目的是提供一种方便的核酸分析方法，其使得能够以高全面性和至少四位数的动态范围共同分析1000种或更多种类型的核酸。 特别地，提供一种非常有效的分析方法，特别是对于非翻译的RNA和微RNA，其靶核酸的类型为10000或更低。 本发明通过以下步骤能够以单分子灵敏度和分辨率的高全面性和定量性能方便快速地分析核酸：一次一个分子制备一组靶核酸片段，并与核酸分子杂交 已知碱基序列并已用荧光物质标记，与靶核酸片段组一起检测标记杂交核酸分子的荧光物质。
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公开(公告)号：EP2623960A1
公开(公告)日：2013-08-07
申请号：EP11828589.9
申请日：2011-07-21
发明人： OZAWA Satoshi , ANAZAWA, Takashi , AKAHORI, Rena , TAKAHASHI, Satoshi , OHURA, Takeshi , KIGUCHI, Masashi
摘要： The present invention provides a device and method for analyzing the characteristics of a biopolymer with excellent mechanical stability, high spatial resolution and sensitivity using a simple device construction. Specifically, the Raman scattered light of a biopolymer is measured and the properties of monomer units forming the biopolymer are analyzed by using a biopolymer property analysis chip (100a) characterized by comprising: a solid substrate (110); at least one nanopore (120) disposed in the solid substrate (110); and one or more electrically conductive thin films (130a, 1 30b) disposed on the solid substrate (110). The biopolymer property analysis chip (100a) is characterized in that the electrically conductive thin films (130a,130b) are disposed partially on the solid substrate (110) where the nanopore (120) is formed and a biopolymer which has penetrated into the nanopore (120) is caused to generate Raman scattered light by means of irradiation with external light.
摘要翻译： 本发明提供了使用简单的装置构造来分析具有优异的机械稳定性，高空间分辨率和灵敏度的生物聚合物的特性的装置和方法。 具体而言，测量生物聚合物的拉曼散射光，并且通过使用生物聚合物性质分析芯片（100a）分析形成生物聚合物的单体单元的性质，所述生物聚合物性质分析芯片（100a）包括：固体基底（110）; 布置在所述固体基底（110）中的至少一个纳米孔（120）; 和设置在固体基底（110）上的一个或多个导电薄膜（130a，130b）。 生物聚合物特性分析芯片（100a）的特征在于，导电薄膜（130a，130b）部分地设置在形成有纳米孔（120）的固体基板（110）上，并且已经渗透到纳米孔中的生物聚合物 120）通过外部光的照射而产生拉曼散射光。
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公开(公告)号：EP2765424B1
公开(公告)日：2018-12-05
申请号：EP12838806.3
申请日：2012-10-04
发明人： SAITO, Toshiro , HAMASAKI, Koshin , TAKAHASHI, Satoshi , MAESHIMA, Muneo , IMAI, Kyoko , IMAI, Kazumichi , TAO, Ryuji
IPC分类号： G01N33/553 , C12M1/00 , C12Q1/68 , G01N21/64 , G01N33/543
CPC分类号： C12Q1/6834 , C12Q1/6804 , C12Q1/6837 , G01N21/6428 , G01N21/6452 , G01N33/54326 , G01N33/54346 , G01N33/54393 , G01N33/587 , G01N2021/6421 , C12Q2525/161 , C12Q2545/114 , C12Q2563/107 , C12Q2563/143 , C12Q2563/149 , C12Q2565/514 , C12Q2563/119
摘要： An object of the present invention is to provide a method and means for analyzing biomolecules that can realize, in biomolecule analysis, a wide dynamic range attained by counting the number of biomolecules and rapid analysis. The present invention relates to a method for analyzing biomolecules, comprising the steps of: immobilizing biomolecules 101 to be analyzed on surfaces of magnetic microparticles 108; reacting labeled probe molecules 104 with the biomolecules 101 to be analyzed; collecting and immobilizing the microparticles 108 on a support substrate 110; and measuring a label on the support substrate 110. Since single-molecule immobilized magnetic microparticles are used in the present invention, the number of biomolecules can be counted, and since hybridization and an antigen-antibody reaction are performed with the microparticles having biomolecules immobilized thereon dispersed, the reaction can be rapidly performed. Further, the type and the abundance of biomolecules of interest can be determined at a single molecular level, so as to evaluate, in particular, the absolute concentration of biomolecules.
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公开(公告)号：EP2735618B1
公开(公告)日：2017-12-06
申请号：EP12815243.6
申请日：2012-05-16
发明人： SAITO, Toshiro , HAMASAKI, Koshin , TAKAHASHI, Satoshi , MAESHIMA, Muneo , IMAI, Kyoko , IMAI, Kazumichi , TAO, Ryuji
CPC分类号： C12Q1/6834 , C12N15/1065 , C12Q2525/207 , C12Q2537/125 , C12Q2537/143 , C12Q2563/143 , C12Q2563/149
摘要： The object of the present invention is to provide a convenient method for nucleic acid analysis, which enables 1000 or more types of nucleic acid to be analyzed collectively with high comprehensiveness and with a dynamic range of at least four digits. In particular, provide is a very effective analytical method especially for untranslated RNAs and microRNAs, of which the types of target nucleic acids is 10000 or lower. The present invention enables nucleic acids to be analyzed conveniently and rapidly with high comprehensiveness and quantitative performance at single-molecule sensitivity and resolution by following the steps of: preparing a group of target nucleic acid fragments one molecule at a time and hybridizing the nucleic acid molecules, which have known base sequences and have been labeled with the fluorescence substances, with the group of the target nucleic acid fragments to detect the fluorescence substances labeling the hybridized nucleic acid molecules.
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8.发明公开SINGLE MOLECULE REAL TIME SEQUENCER, NUCLEIC ACID ANALYZER AND SINGLE MOLECULE REAL TIME SEQUENCING METHOD 有权单分子实时测序仪，核酸分析仪和单分子实时测序方法
公开(公告)号：EP2292727A1
公开(公告)日：2011-03-09
申请号：EP09770037.1
申请日：2009-06-08
CPC分类号： C12Q1/6874 , B01J2219/00529 , B01J2219/00596 , B01J2219/00608 , B01J2219/00711 , B01J2219/00722 , B01L3/502707 , B82Y30/00 , G01N21/648 , C12Q2523/313 , C12Q2523/319 , C12Q2561/113 , C12Q2565/628 , C12Q2525/186 , C12Q2563/107 , C12Q2565/631
摘要： An object of the present invention relates to selectively control an extension reaction within a desired area in a substrate. In the present invention, an oligo probe arranged with a caged compound at the terminal thereof is immobilized to a reaction field area in the substrate. After pouring a reaction solution into a flow cell including the reaction field area, the reaction field area alone is irradiated with light to associate the photodegradation-active protecting group at the terminal of the oligo probe that has been immobilized in the reaction field area and thus to selectively control initiation of a polymerase extension reaction. In the flow cell, a plural number of the reaction field areas are arranged at constant interval on the substrate. The flow cell immobilized to a moving stage is moved by a distance equal to the interval between the adjacent reaction field areas and then light irradiation is carried out to measure the extension reaction continuously. By repeating these operations, the base extension reaction is stably measured in the flow cell without using complicated channels or without replacing the reaction solution.
摘要翻译： 本发明的一个目的涉及选择性地控制衬底中期望区域内的延伸反应。 在本发明中，将在其末端布置有笼状化合物的寡核苷酸探针固定到基底中的反应场区域。 在将反应溶液注入到包含反应场区域的流通池中之后，单独对反应场区域照射光以使已固定在反应场区域中的寡聚物探针的末端处的光降解活性保护基团缔合 以选择性地控制聚合酶延伸反应的启动。 在流通池中，多个反应场区以恒定的间隔排列在衬底上。 固定在移动台上的流动池移动与相邻反应场区之间的间隔相等的距离，然后进行光照以连续测量延伸反应。 通过重复这些操作，在流通池中稳定地测量碱扩展反应而不使用复杂的通道或不更换反应溶液。
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