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公开(公告)号：EP2019309B1
公开(公告)日：2013-04-24
申请号：EP08012794.7
申请日：2008-07-15
CPC分类号： G01N21/648 , G01N21/6428 , G01N21/6452 , G01N2021/058 , G01N2021/6439 , Y10S435/808
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2.发明公开
公开(公告)号：EP2040060A1
公开(公告)日：2009-03-25
申请号：EP08016588.9
申请日：2008-09-19
CPC分类号： G01N21/648 , G01N21/6428
摘要： An object of the present invention relates to detecting a target substance with high contrast. The invention relates to analysis of a target substance using a light-transmitting substrate and a metal for inducing plasmon resonance, and further using a low refractive index layer with an opening portion, which forms an interface with the substrate, and which has a lower refractive index than the substrate. Light emitted from a substrate side is totally reflected at the interface to irradiate the metal arranged in the opening portion with evanescent light. Light generated from the target substance by plasmon resonance of the evanescent light is detected. According to the invention, the radiation of evanescent light to a martial other than the target substance can be reduced, and thereby light emission from the martial other than the target substance, e.g., a molecule floating around the target substance, can be reduced.
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公开(公告)号：EP2423672A1
公开(公告)日：2012-02-29
申请号：EP11008104.9
申请日：2008-07-15
CPC分类号： G01N21/648 , G01N21/6428 , G01N21/6452 , G01N2021/058 , G01N2021/6439 , Y10S435/808
摘要： The present invention relates to a nucleic acid analysis device for analysis of nucleic acid in a sample through fluorometry, in which a localized surface plasmon is generated by light irradiation, and in which a nucleic acid probe or a nucleic acid synthase (605) for the analysis of the nucleic acid in the sample is disposed in a region of generation of the surface plasmon. The present invention allows the fluorescence intensifying effect of the surface plasmon to be produced efficiently and also enables the immobilization of a DNA probe or the nucleic acid synthase in a region on which the fluorescence intensifying effect is exerted, thus making it possible to carry out a measurement on the base elongation reaction without having to remove the unreacted substrate with the fluorescent molecule (102). The device comprises a flat supporting substrate (103),(301) and a metal structure having a vertex which protrudes through a thin film layer whereby a nucleic acid probe or synthase (307) is provided at the vertex.
摘要翻译： 本发明涉及一种用于通过荧光测定分析样品中的核酸的核酸分析装置，其中通过光照射产生局部表面等离子体激元，并且其中核酸探针或核酸合成酶（605）用于 将样品中核酸的分析置于表面等离子体的产生区域中。 本发明允许有效地产生表面等离子体激元的荧光增强效果，并且还能够在发挥荧光增强作用的区域中固定DNA探针或核酸合酶，从而可以进行 测量基础伸长反应而不用荧光分子除去未反应的底物（102）。 该装置包括平坦支撑基板（103），（301）和具有突出穿过薄膜层的顶点的金属结构，由此在顶点设置有核酸探针或合酶（307）。
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公开(公告)号：EP2019309A3
公开(公告)日：2009-03-11
申请号：EP08012794.7
申请日：2008-07-15
CPC分类号： G01N21/648 , G01N21/6428 , G01N21/6452 , G01N2021/058 , G01N2021/6439 , Y10S435/808
摘要： The present invention relates to a nucleic acid analysis device for analysis of nucleic acid in a sample through fluorometry, in which a localized surface plasmon is generated by light irradiation, and in which a nucleic acid probe or a nucleic acid synthase for the analysis of the nucleic acid in the sample is disposed in a region of generation of the surface plasmon. The present invention allows the fluorescence intensifying effect of the surface plasmon to be produced efficiently and also enables the immobilization of a DNA probe or the nucleic acid synthase in a region on which the fluorescence intensifying effect is exerted, thus making it possible to carry out a measurement on the base elongation reaction without having to remove the unreacted substrate with the fluorescent molecule.
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5.发明授权Apparatus and method for detecting a target substance 有权Vorrichtung und Verfahren zur Detektion einer Zielsubstanz
公开(公告)号：EP2040060B1
公开(公告)日：2017-04-19
申请号：EP08016588.9
申请日：2008-09-19
CPC分类号： G01N21/648 , G01N21/6428
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公开(公告)号：EP2423672B1
公开(公告)日：2013-07-03
申请号：EP11008104.9
申请日：2008-07-15
CPC分类号： G01N21/648 , G01N21/6428 , G01N21/6452 , G01N2021/058 , G01N2021/6439 , Y10S435/808
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公开(公告)号：EP2019309A2
公开(公告)日：2009-01-28
申请号：EP08012794.7
申请日：2008-07-15
CPC分类号： G01N21/648 , G01N21/6428 , G01N21/6452 , G01N2021/058 , G01N2021/6439 , Y10S435/808
摘要： The present invention relates to a nucleic acid analysis device for analysis of nucleic acid in a sample through fluorometry, in which a localized surface plasmon is generated by light irradiation, and in which a nucleic acid probe or a nucleic acid synthase for the analysis of the nucleic acid in the sample is disposed in a region of generation of the surface plasmon. The present invention allows the fluorescence intensifying effect of the surface plasmon to be produced efficiently and also enables the immobilization of a DNA probe or the nucleic acid synthase in a region on which the fluorescence intensifying effect is exerted, thus making it possible to carry out a measurement on the base elongation reaction without having to remove the unreacted substrate with the fluorescent molecule.
摘要翻译： 本发明涉及用于通过荧光测定分析样品中的核酸的核酸分析装置，其中通过光照射产生局部表面等离子体激元，并且其中用于分析所述核酸探针或核酸合酶 将样品中的核酸置于产生表面等离子体的区域中。 本发明允许有效地产生表面等离子体激元的荧光增强效果，并且还能够在发挥荧光增强作用的区域中固定DNA探针或核酸合酶，从而可以进行 基本伸长反应的测量，而不必用荧光分子除去未反应的底物。
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