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1.发明授权Apparatus and method for detecting a target substance 有权Vorrichtung und Verfahren zur Detektion einer Zielsubstanz
公开(公告)号：EP2040060B1
公开(公告)日：2017-04-19
申请号：EP08016588.9
申请日：2008-09-19
CPC分类号： G01N21/648 , G01N21/6428
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公开(公告)号：EP2423672B1
公开(公告)日：2013-07-03
申请号：EP11008104.9
申请日：2008-07-15
CPC分类号： G01N21/648 , G01N21/6428 , G01N21/6452 , G01N2021/058 , G01N2021/6439 , Y10S435/808
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公开(公告)号：EP2019309A2
公开(公告)日：2009-01-28
申请号：EP08012794.7
申请日：2008-07-15
CPC分类号： G01N21/648 , G01N21/6428 , G01N21/6452 , G01N2021/058 , G01N2021/6439 , Y10S435/808
摘要： The present invention relates to a nucleic acid analysis device for analysis of nucleic acid in a sample through fluorometry, in which a localized surface plasmon is generated by light irradiation, and in which a nucleic acid probe or a nucleic acid synthase for the analysis of the nucleic acid in the sample is disposed in a region of generation of the surface plasmon. The present invention allows the fluorescence intensifying effect of the surface plasmon to be produced efficiently and also enables the immobilization of a DNA probe or the nucleic acid synthase in a region on which the fluorescence intensifying effect is exerted, thus making it possible to carry out a measurement on the base elongation reaction without having to remove the unreacted substrate with the fluorescent molecule.
摘要翻译： 本发明涉及用于通过荧光测定分析样品中的核酸的核酸分析装置，其中通过光照射产生局部表面等离子体激元，并且其中用于分析所述核酸探针或核酸合酶 将样品中的核酸置于产生表面等离子体的区域中。 本发明允许有效地产生表面等离子体激元的荧光增强效果，并且还能够在发挥荧光增强作用的区域中固定DNA探针或核酸合酶，从而可以进行 基本伸长反应的测量，而不必用荧光分子除去未反应的底物。
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4.发明公开Detection method and detection apparatus of substance in biological sample 有权探测器中的Verfahren und Vorrichtung zum Nachweis einer Substanz
公开(公告)号：EP1584917A2
公开(公告)日：2005-10-12
申请号：EP05007660.3
申请日：2005-04-07
发明人： Takahashi, Satoshi
CPC分类号： G01N21/6428 , G01N21/07 , G01N21/645 , G01N2021/6421 , G01N2021/6491
摘要： A high accuracy method for detecting a biological-related substance is provided by which an abnormal state, such as the adhesion of dust, the reduction of a sample solution, or the like can be judged. Means for detecting a light emitted by the portion for light detection after dividing the light into at least a plurality of wavelength zones is provided. One of the plurality of wavelength zones includes substantially the same wavelength zone as that of the component of the excitation light. The light intensity of the component of the excitation light is detected and it is compared with a predetermined intensity (threshold). A highly accurate fluorescence measurement can be realized, by which the abnormality of a sample can be judged.
摘要翻译： 提供了用于检测生物相关物质的高精度方法，通过该方法可以判断诸如灰尘附着，样品溶液的还原等异常状态。 提供了用于在将光分成至少多个波长区域之后检测由光检测部分发出的光的装置。 多个波长区域中的一个包括与激发光的分量基本上相同的波长区域。 检测激发光的分量的光强度，并将其与预定的强度（阈值）进行比较。 可以实现高精度的荧光测定，能够判断样品的异常。
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5.发明公开
公开(公告)号：EP2040060A1
公开(公告)日：2009-03-25
申请号：EP08016588.9
申请日：2008-09-19
CPC分类号： G01N21/648 , G01N21/6428
摘要： An object of the present invention relates to detecting a target substance with high contrast. The invention relates to analysis of a target substance using a light-transmitting substrate and a metal for inducing plasmon resonance, and further using a low refractive index layer with an opening portion, which forms an interface with the substrate, and which has a lower refractive index than the substrate. Light emitted from a substrate side is totally reflected at the interface to irradiate the metal arranged in the opening portion with evanescent light. Light generated from the target substance by plasmon resonance of the evanescent light is detected. According to the invention, the radiation of evanescent light to a martial other than the target substance can be reduced, and thereby light emission from the martial other than the target substance, e.g., a molecule floating around the target substance, can be reduced.
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6.发明公开
公开(公告)号：EP1584917A3
公开(公告)日：2008-01-30
申请号：EP05007660.3
申请日：2005-04-07
发明人： Takahashi, Satoshi
CPC分类号： G01N21/6428 , G01N21/07 , G01N21/645 , G01N2021/6421 , G01N2021/6491
摘要： A high accuracy method for detecting a biological-related substance is provided by which an abnormal state, such as the adhesion of dust, the reduction of a sample solution, or the like can be judged. Means for detecting a light emitted by the portion for light detection after dividing the light into at least a plurality of wavelength zones is provided. One of the plurality of wavelength zones includes substantially the same wavelength zone as that of the component of the excitation light. The light intensity of the component of the excitation light is detected and it is compared with a predetermined intensity (threshold). A highly accurate fluorescence measurement can be realized, by which the abnormality of a sample can be judged.
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公开(公告)号：EP2019309B1
公开(公告)日：2013-04-24
申请号：EP08012794.7
申请日：2008-07-15
CPC分类号： G01N21/648 , G01N21/6428 , G01N21/6452 , G01N2021/058 , G01N2021/6439 , Y10S435/808
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公开(公告)号：EP2063258A3
公开(公告)日：2010-12-01
申请号：EP08020249.2
申请日：2008-11-20
IPC分类号： G01N21/64
CPC分类号： G01N21/648 , G01N21/6456 , G01N2021/6423
摘要： A metallic structure is provided on a surface of a substrate. A component having a longer wavelength than excitation light is detected from luminescence from fixation positions of biomolecules and emitted from a material other than the biomolecules, and is used for photometrical analysis. As the structure, usable is a particulate (a metallic structure of a size not larger than a wavelength of the excitation light), a minute protrusion, or a thin film with minute apertures, which are made of a metal such as gold, chrome, silver or aluminum. In the case of the particulate or the minute protrusion, photoluminescence of the structure is detected with a biomolecule being fixed thereon. In the case of the thin film with minute apertures, Raman scattered light of specimen solution around the biomolecules, and photoluminescence of the metallic structure near the biomolecules are detected with biomolecules being fixed in the apertures.
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公开(公告)号：EP2063258A2
公开(公告)日：2009-05-27
申请号：EP08020249.2
申请日：2008-11-20
IPC分类号： G01N21/64
CPC分类号： G01N21/648 , G01N21/6456 , G01N2021/6423
摘要： A metallic structure is provided on a surface of a substrate. A component having a longer wavelength than excitation light is detected from luminescence from fixation positions of biomolecules and emitted from a material other than the biomolecules, and is used for photometrical analysis. As the structure, usable is a particulate (a metallic structure of a size not larger than a wavelength of the excitation light), a minute protrusion, or a thin film with minute apertures, which are made of a metal such as gold, chrome, silver or aluminum. In the case of the particulate or the minute protrusion, photoluminescence of the structure is detected with a biomolecule being fixed thereon. In the case of the thin film with minute apertures, Raman scattered light of specimen solution around the biomolecules, and photoluminescence of the metallic structure near the biomolecules are detected with biomolecules being fixed in the apertures.
摘要翻译： 金属结构设置在基板的表面上。 从生物分子的固定位置的发光和从生物分子以外的材料发射的发光检测出具有比激发光更长波长的成分，并用于光度分析。 作为结构，可以使用由金，铬等金属制成的颗粒（不大于激发光的波长的金属结构），微小突起或微小孔的薄膜， 银或铝。 在颗粒或微小突起的情况下，用固定有生物分子的方法检测结构的光致发光。 在具有微小孔的薄膜的情况下，拉曼散射生物分子周围的样品溶液的光，并且用生物分子固定在孔中来检测生物分子附近的金属结构的光致发光。
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公开(公告)号：EP2423672A1
公开(公告)日：2012-02-29
申请号：EP11008104.9
申请日：2008-07-15
CPC分类号： G01N21/648 , G01N21/6428 , G01N21/6452 , G01N2021/058 , G01N2021/6439 , Y10S435/808
摘要： The present invention relates to a nucleic acid analysis device for analysis of nucleic acid in a sample through fluorometry, in which a localized surface plasmon is generated by light irradiation, and in which a nucleic acid probe or a nucleic acid synthase (605) for the analysis of the nucleic acid in the sample is disposed in a region of generation of the surface plasmon. The present invention allows the fluorescence intensifying effect of the surface plasmon to be produced efficiently and also enables the immobilization of a DNA probe or the nucleic acid synthase in a region on which the fluorescence intensifying effect is exerted, thus making it possible to carry out a measurement on the base elongation reaction without having to remove the unreacted substrate with the fluorescent molecule (102). The device comprises a flat supporting substrate (103),(301) and a metal structure having a vertex which protrudes through a thin film layer whereby a nucleic acid probe or synthase (307) is provided at the vertex.
摘要翻译： 本发明涉及一种用于通过荧光测定分析样品中的核酸的核酸分析装置，其中通过光照射产生局部表面等离子体激元，并且其中核酸探针或核酸合成酶（605）用于 将样品中核酸的分析置于表面等离子体的产生区域中。 本发明允许有效地产生表面等离子体激元的荧光增强效果，并且还能够在发挥荧光增强作用的区域中固定DNA探针或核酸合酶，从而可以进行 测量基础伸长反应而不用荧光分子除去未反应的底物（102）。 该装置包括平坦支撑基板（103），（301）和具有突出穿过薄膜层的顶点的金属结构，由此在顶点设置有核酸探针或合酶（307）。
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