公开(公告)号：EP0915168A1

公开(公告)日：1999-05-12

申请号：EP97919717.5

申请日：1997-04-28

申请人： Hoechst Marion Roussel, Ltd.

发明人： TAKAHASHI, Mikiko ,  MAKISHIMA, F. Hoechst Marion Roussel Ltd ,  KIMURA, Michio Hoechst Marion Roussel Ltd

IPC分类号： C12P21/02 ,  C12N15/12

CPC分类号： C07K14/51 ,  A61K38/00

摘要： A process for producing a muturation bone morphogenetic protein by the action of a processing enzyme on a bone morphogenetic protein precursor, which comprisis either introducing an expression vector of a bone morphogenetic protein precursor and an expression vector of a processing enzyme into an animal cell strains, culturing the resultant strain to yield a maturation bone morphogenietc protein, and separating the same from the culture, or alternatively adding a solution of a processing enzyme to a solution of a bone morphogenetic protein precursor and incubating the obtained solution mixture. A suitable process comprises introducing an expression vector of a human MP52 precusor and an expression vector of a secretory furin variant into an established animal cell strain, culturing the resultant strain to yield a muturation bone morphogenetic protein, and separating the same from the culture.

摘要翻译： 通过加工酶对骨形态发生蛋白前体的作用产生变性骨形态发生蛋白的方法，其包括将骨形态发生蛋白前体的表达载体和加工酶的表达载体引入动物细胞株， 培养所得菌株以产生成熟骨形态发生蛋白，并将其从培养物中分离，或者将加工酶的溶液加入到骨形态发生蛋白前体的溶液中，并将所得溶液混合物孵育。 合适的方法包括将人MP52前体细胞的表达载体和分泌性弗林蛋白酶变体的表达载体导入建立的动物细胞株，培养所得菌株以产生变性骨形态发生蛋白，并将其与培养物分离。