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    METHOD OF PRODUCING PLASTID TRANSFORMANTS
    1.
    发明公开
    METHOD OF PRODUCING PLASTID TRANSFORMANTS 审中-公开
    生产PLASTID TRANSFORMANT的方法

    公开(公告)号：EP3180436A2

    公开(公告)日：2017-06-21

    申请号：EP16778901.5

    申请日：2016-08-24

    申请人： Honda Motor Co., Ltd.

    发明人： YAMAGUCHI, Asuka ,  OKUMA, Jiro ,  KONDO, Yasuhiro ,  HIROSE, Yoshitsugu

    IPC分类号： C12N15/82

    CPC分类号： C12N15/8209 ,  C12N15/821 ,  C12N15/8214

    摘要： A method of producing plastid transformants including a process (a) in which genes coding for proteins having a function of neutralizing nitrite toxicity are introduced into plant tissues and a process (b) in which the plant tissues into which the genes are inserted in the process (a) are cultured in a medium containing nitrite nitrogen having a concentration at which growth of wild type plant cells is suppressed or a concentration at which regeneration of wild type plants is suppressed and thus plastid transformants obtained by inserting the genes into plastid genomes are screened.

    摘要翻译： （a）中，将编码具有中和亚硝酸毒性功能的蛋白质的基因导入植物组织的方法（a），以及在该方法中插入了基因的植物组织的方法（b），制备质体转化体的方法 （a）在含有野生型植物细胞生长被抑制的浓度的亚硝酸盐氮的培养基或抑制野生型植物再生的浓度的培养基中培养，从而筛选出通过将该基因插入到质体基因组中获得的质体转化体 。

    THERMOSTABLE GLYCOSIDE HYDROLASE
    2.
    发明公开
    THERMOSTABLE GLYCOSIDE HYDROLASE 审中-公开
    热稳定性胶体酶

    公开(公告)号：EP3095858A1

    公开(公告)日：2016-11-23

    申请号：EP16170320.2

    申请日：2016-05-19

    申请人： Honda Motor Co., Ltd.

    发明人： SUDA, Migiwa ,  OKUMA, Jiro ,  YAMAGUCHI, Asuka ,  HIROSE, Yoshitsugu ,  KONDO, Yasuhiro ,  SATO, Masaru ,  SHIBATA, Daisuke

    IPC分类号： C12N9/42

    CPC分类号： C12N9/2437 ,  C12P19/02 ,  C12P19/12 ,  C12P19/14 ,  C12Y302/01004

    摘要： A thermostable glycoside hydrolase having a glycoside hydrolase catalytic domain including: (A) a polypeptide including the amino acid sequence represented by SEQ ID NO: 1; (B) a polypeptide including an amino acid sequence in which at least one amino acid has been deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1, and having hydrolysis activity against a substrate of PSA and hydrolysis activity against a substrate of CMC at least under conditions of 65°C and pH 4.0; (C) a polypeptide including an amino acid sequence having 80% or greater sequence identity with the amino acid sequence represented by SEQ ID NO: 1, and having hydrolysis activity against a substrate of PSA and hydrolysis activity against a substrate of CMC at least under conditions of 65°C and pH 4.0; (D) a polypeptide in which are linked, either directly or via a linker, a polypeptide including an amino acid sequence in which at least one amino acid has been deleted, substituted, or added in the region composed of amino acids from positions 51 to 485 in the amino acid sequence represented by SEQ ID NO: 1, and having hydrolysis activity against a substrate of PSA at least under conditions of 65°C and pH 4.0, and a polypeptide including an amino acid sequence in which at least one amino acid has been deleted, substituted, or added in the region composed of amino acids from positions 530 to 823 in the amino acid sequence represented by SEQ ID NO: 1, and having hydrolysis activity against a substrate of CMC at least under conditions of 65°C and pH 4.0; or (E) a polypeptide in which are linked, either directly or via a linker, a polypeptide including an amino acid sequence having 80% or greater sequence identity with the region composed of amino acids from position 51 to position 485 in the amino acid sequence represented by SEQ ID NO: 1 and having hydrolysis activity against a substrate of PSA at least under conditions of 65°C and pH 4.0, and a polypeptide including an amino acid sequence having 80% or greater sequence identity with the region composed of amino acids from positions 530 to 823 in the amino acid sequence represented by SEQ ID NO: 1 and having hydrolysis activity against a substrate of CMC at least under conditions of 65°C and pH 4.0.

    摘要翻译： 一种具有糖苷水解酶催化结构域的热稳定型糖苷水解酶，包括：（A）包含SEQ ID NO：1所示的氨基酸序列的多肽; （B）包含氨基酸序列的多肽，其中至少一个氨基酸已被缺失，取代或加入到由SEQ ID NO：1表示的氨基酸序列中，并且具有针对PSA底物的水解活性和水解活性 至少在65℃和pH4.0的条件下相对于CMC的底物; （C）多肽，其包含与SEQ ID NO：1所示的氨基酸序列具有80％以上序列同一性的氨基酸序列，并且具有针对PSA底物的水解活性和对CMC底物的水解活性，至少在 条件为65℃和pH 4.0; （D）多肽，其直接或经由连接体连接多肽，所述多肽包括氨基酸序列，其中至少一个氨基酸已被缺失，取代或添加在由位置51至 485，并且至少在65℃和pH4.0的条件下具有针对PSA底物的水解活性，以及​​包含氨基酸序列的多肽，其中至少一个氨基酸 已经在由SEQ ID NO：1所示的氨基酸序列中的位置530至823的氨基酸组成的区域中被删除，取代或添加，并且至少在65℃的条件下对CMC的底物具有水解活性 和pH 4.0; 或（E）多肽，其直接地或经由连接体连接多肽，所述多肽包括与氨基酸序列中从位置51至位置485的氨基酸组成的区域具有80％以上序列同一性的氨基酸序列 由SEQ ID NO：1表示，并且至少在65℃和pH4.0的条件下具有针对PSA底物的水解活性，以及​​包含与由氨基酸组成的区域具有80％以上序列同一性的氨基酸序列的多肽 由SEQ ID NO：1表示的氨基酸序列中的位置530至823，并且至少在65℃和pH4.0的条件下对CMC的底物具有水解活性。