公开(公告)号：EP0713088B1

公开(公告)日：1999-07-14

申请号：EP96200207.7

申请日：1992-12-09

申请人： I.S.S. (U.S.A.) Inc.

发明人： Barbieri, Beniamino ,  Gratton, Enrico

IPC分类号： G01N21/64

CPC分类号： G01N21/6408

公开(公告)号：EP0713088A2

公开(公告)日：1996-05-22

申请号：EP96200207.7

申请日：1992-12-09

申请人： I.S.S. (U.S.A.) Inc.

发明人： Barbieri, Beniamino ,  Gratton, Enrico

IPC分类号： G01N21/64

CPC分类号： G01N21/6408

摘要： Apparatus for cross-correlation frequency domain fluorometry and/or phosphorimetry in which means are provided for sequentially performing runs of the cross correlation frequency domain fluorometry and/or phosphorimetry at sequentially differing first and second frequencies. The intensities of signal responses of the runs are detected at the respective cross-correlation frequency in each run. The detection of the signal response is prolonged in each run until an integrated signal with a specified standard deviation has been acquired at each of the differing runs. Preferably the sequential runs are automatically executed by a program. Also, the waveforms sensed by deriving the resultant signal response in each run are folded. That is: corresponding segments of the waveforms are superimposed to obtain an average waveform value for each run having an increased signal to noise ratio over the individual waveform segments. Also, preferably, a software-created variable frequency digital filter is used to filter signal responses at the cross-correlation frequency.

摘要翻译： 用于互相关频域荧光测定和/或荧光测定的装置，其中提供了用于在顺序地不同的第一和第二频率顺序地执行互相关频域荧光测定和/或磷酸测定的方法。 在每次运行中，以相应的互相关频率检测运行的信号响应的强度。 信号响应的检测在每次运行中延长，直到在每个不同的运行中已经获得具有指定的标准偏差的积分信号。 优选地，顺序运行由程序自动执行。 此外，通过导出每次运行中的结果信号响应而感测的波形被折叠。 也就是说，叠加相应的波形段以获得每个波形的平均波形值，该平均波形值相对于各个波形段具有增加的信噪比。 此外，优选地，软件创建的可变频数字滤波器用于在互相关频率处对信号响应进行滤波。

公开(公告)号：EP0713088A3

公开(公告)日：1996-06-05

申请号：EP96200207.7

申请日：1992-12-09

申请人： I.S.S. (U.S.A.) Inc.

发明人： Barbieri, Beniamino ,  Gratton, Enrico

IPC分类号： G01N21/64

CPC分类号： G01N21/6408

摘要： Apparatus for cross-correlation frequency domain fluorometry and/or phosphorimetry in which means are provided for sequentially performing runs of the cross correlation frequency domain fluorometry and/or phosphorimetry at sequentially differing first and second frequencies. The intensities of signal responses of the runs are detected at the respective cross-correlation frequency in each run. The detection of the signal response is prolonged in each run until an integrated signal with a specified standard deviation has been acquired at each of the differing runs. Preferably the sequential runs are automatically executed by a program. Also, the waveforms sensed by deriving the resultant signal response in each run are folded. That is: corresponding segments of the waveforms are superimposed to obtain an average waveform value for each run having an increased signal to noise ratio over the individual waveform segments. Also, preferably, a software-created variable frequency digital filter is used to filter signal responses at the cross-correlation frequency.

摘要翻译： 用于互相关频域荧光测量和/或荧光测量的设备，其中提供了用于在顺序不同的第一和第二频率顺序地执行互相关频域荧光测量和/或磷光测定的运行的装置。 运行的信号响应的强度在每次运行中以相应的互相关频率检测。 每次运行都会延长信号响应的检测时间，直到在每次不同的运行中获得具有指定标准偏差的积分信号。 优选地，顺序运行由程序自动执行。 而且，通过导出每次运行中的结果信号响应而感测的波形被折叠。 即：叠加相应的波形段，以获得在各个波形段上具有增加的信噪比的每个运行的平均波形值。 而且，优选地，使用软件创建的可变频率数字滤波器来以互相关频率过滤信号响应。

公开(公告)号：EP0663591A1

公开(公告)日：1995-07-19

申请号：EP94309361.7

申请日：1994-12-14

申请人： I.S.S. (U.S.A.) Inc. ,  THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS

发明人： Gratton, Enrico ,  Fantini, Sergio ,  Franceschini, Maria Angela ,  Mantulin, William ,  Barbieri, Beniamino ,  Maier, John ,  Walker, Scott A.

IPC分类号： G01N21/47 ,  A61B5/00

CPC分类号： G01N21/314 ,  A61B5/0059 ,  A61B5/14532 ,  A61B5/1455 ,  A61B5/14552 ,  A61B5/6824 ,  A61B2562/0233 ,  A61B2562/046 ,  G01N21/49

摘要： The quantitative determination of various materials in highly scattering media such as living tissue may be determined in an external, photometric manner by the use of a plurality of light sources positioned at differing distances from a sensor. The light from said sources is amplitude modulated, and, in accordance with conventional frequency domain fluorometry or phosphorimetry techniques, the gain of the sensor is modulated at a frequency different from the frequency of the light modulation. Data may be acquired from each of the light sources at differing distances at a frequency which is the difference between the two frequencies described above. From these sets of data from each individual light source, curves may be constructed, and the slopes used to quantitatively determine the amount of certain materials present, for example oxyhemoglobin and deoxyhemoglobin in living tissue, or to determine at least the relative concentration of a material such as glucose.

摘要翻译： 可以通过使用位于与传感器不同的距离的多个光源以外部的光度方式来确定诸如活组织的高度散射介质中的各种材料的定量测定。 来自所述源的光被幅度调制，并且根据常规的频域荧光测定或荧光测定技术，传感器的增益以与光调制频率不同的频率被调制。 可以以与上述两个频率之间的差异的频率以不同的距离从每个光源获取数据。 根据来自每个单独光源的这些数据集，可以构建曲线，并且用于定量确定存在的某些材料的量的斜率，例如活组织中的氧合血红蛋白和脱氧血红蛋白，或至少确定材料的相对浓度 如葡萄糖。

公开(公告)号：EP1630547A1

公开(公告)日：2006-03-01

申请号：EP05077523.8

申请日：2001-07-18

申请人： I.S.S. (U.S.A.) Inc.

发明人： Barbieri, Beniamino ,  Gratton, Enrico

IPC分类号： G01N21/64 ,  G01N21/25

CPC分类号： G01N21/6452 ,  G01N21/6408 ,  G01N2021/6417

摘要： This inventiori provides a method of performing fluorescence correlation spectroscope which comprises acquiring a fluorescence measurement from a sample over time to obtain a histogram of photon counts; deriving the molecular brightness of at least one molecular species in the sample; and determining the concentration of the species.

摘要翻译： 本发明提供了一种执行荧​​光相关分析仪的方法，其包括从样品随时间获取荧光测量以获得光子计数的直方图; 得出样品中至少一种分子物质的分子亮度; 并确定物种的浓度。

公开(公告)号：EP1174706A2

公开(公告)日：2002-01-23

申请号：EP01306195.7

申请日：2001-07-18

申请人： I.S.S. (U.S.A.) Inc.

发明人： Barbieri, Beniamino ,  Gratton, Enrico

IPC分类号： G01N21/64

CPC分类号： G01N21/6452 ,  G01N21/6408 ,  G01N2021/6417

摘要： A fluorescence spectrometer comprises a laser (12) and at least one beam splitter (18) positioned to receive a light beam from the laser and to divide it into several first light beam portions (16). Dichroic mirrors (17) are positioned to separately receive the first light beam portions and to reflect the beam portions at an angle to the first light beam portions. The first light beam portions respectively enter different spectometer units (20a-20d) for the irradiation of separate samples and sensing fluorescence emitted from the samples. Transparent chambers are provided for holding the samples. Objective lens systems are respectively positioned in the path of the reflected beam portions to respectively focus each reflected beam portion to a point within one of the separate transparent chambers. Lenses are positioned to receive fluorescence from a sample for testing within the transparent chambers and to respectively focus the fluorescence at pin holes in opaque partitions. The lenses are positioned to receive the fluorescence, which passes back through the objective lens system and the dichroic mirror. Light detectors are each respectively positioned adjacent to one of the partitions, with one of the partitions respectively positioned between each of the lens and the light detectors to permit each light detector to sense fluorescence through the pin hole. Electronics are provided to receive and process signals from each light detector. Structure is provided to permit high speed data collection from a large number of samples in separate, transparent chambers.

摘要翻译： 荧光光谱仪包括激光器（12）和至少一个分光器（18），其被定位成接收来自激光器的光束并将其分成几个第一光束部分（16）。 分色镜（17）被定位成分别容纳第一光束部分并且将光束部分以与第一光束部分成一定角度的方式反射。 第一光束部分分别进入不同的光谱仪单元（20a-20d），用于照射单独的样品并感测从样品发出的荧光。 提供透明室以保持样品。 物镜系统分别位于反射光束部分的路径中，以将每个反射光束部分分别聚焦到分离的透明室之一内的点。 透镜被定位为接收来自样品的荧光以在透明室内进行测试，并且将荧光分别聚焦在不透明分区中的针孔处。 透镜被定位成接收荧光，其通过物镜系统和分色镜返回。 光检测器各自分别定位成与其中一个隔板相邻，其中一个隔板分别位于透镜和光检测器之间，以允许每个光检测器通过销孔感测荧光。 电子设备用于接收和处理来自每个光检测器的信号。 提供结构以允许从分离的透明室中的大量样品收集高速数据。

公开(公告)号：EP1174706A3

公开(公告)日：2004-07-07

申请号：EP01306195.7

申请日：2001-07-18

申请人： I.S.S. (U.S.A.) Inc.

发明人： Barbieri, Beniamino ,  Gratton, Enrico

IPC分类号： G01N21/64 ,  G01N21/25

CPC分类号： G01N21/6452 ,  G01N21/6408 ,  G01N2021/6417

摘要： A fluorescence spectrometer comprises a laser (12) and at least one beam splitter (18) positioned to receive a light beam from the laser and to divide it into several first light beam portions (16). Dichroic mirrors (17) are positioned to separately receive the first light beam portions and to reflect the beam portions at an angle to the first light beam portions. The first light beam portions respectively enter different spectometer units (20a-20d) for the irradiation of separate samples and sensing fluorescence emitted from the samples. Transparent chambers are provided for holding the samples. Objective lens systems are respectively positioned in the path of the reflected beam portions to respectively focus each reflected beam portion to a point within one of the separate transparent chambers. Lenses are positioned to receive fluorescence from a sample for testing within the transparent chambers and to respectively focus the fluorescence at pin holes in opaque partitions. The lenses are positioned to receive the fluorescence, which passes back through the objective lens system and the dichroic mirror. Light detectors are each respectively positioned adjacent to one of the partitions, with one of the partitions respectively positioned between each of the lens and the light detectors to permit each light detector to sense fluorescence through the pin hole. Electronics are provided to receive and process signals from each light detector. Structure is provided to permit high speed data collection from a large number of samples in separate, transparent chambers.

公开(公告)号：EP0663591B1

公开(公告)日：2002-06-12

申请号：EP94309361.7

申请日：1994-12-14

申请人： I.S.S. (U.S.A.) Inc. ,  THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS

发明人： Gratton, Enrico ,  Fantini, Sergio ,  Franceschini, Maria Angela ,  Mantulin, William ,  Barbieri, Beniamino ,  Maier, John ,  Walker, Scott A.

IPC分类号： G01N21/47 ,  A61B5/00

CPC分类号： G01N21/314 ,  A61B5/0059 ,  A61B5/14532 ,  A61B5/1455 ,  A61B5/14552 ,  A61B5/6824 ,  A61B2562/0233 ,  A61B2562/046 ,  G01N21/49

公开(公告)号：EP0551736B1

公开(公告)日：1997-03-19

申请号：EP92311236.1

申请日：1992-12-09

申请人： I.S.S. (U.S.A.) Inc.

发明人： Barbieri, Beniamino ,  Gratton, Enrico

IPC分类号： G01N21/64

CPC分类号： G01N21/6408

公开(公告)号：EP0551736A1

公开(公告)日：1993-07-21

申请号：EP92311236.1

申请日：1992-12-09

申请人： I.S.S. (U.S.A.) Inc.

发明人： Barbieri, Beniamino ,  Gratton, Enrico

IPC分类号： G01N21/64

CPC分类号： G01N21/6408

摘要： Apparatus for cross-correlation frequency domain fluorometry and/or phosphorimetry in which means are provided for sequentially performing runs of the cross correlation frequency domain fluorometry and/or phosphorimetry at sequentially differing first and second frequencies. The intensities of signal responses of the runs are detected at the respective cross-correlation frequency in each run. The detection of the signal response is prolonged in each run until an integrated signal with a specified standard deviation has been acquired at each of the differing runs. Preferably the sequential runs are automatically executed by a program. Also, the waveforms sensed by deriving the resultant signal response in each run are folded. That is: corresponding segments of the waveforms are superimposed to obtain an average waveform value for each run having an increased signal to noise ratio over the individual waveform segments. Also, preferably, a software-created variable frequency digital filter is used to filter signal responses at the cross-correlation frequency.

摘要翻译： 用于互相关频域荧光测定和/或荧光测定的装置，其中提供了用于在顺序地不同的第一和第二频率顺序地执行互相关频域荧光测定和/或磷酸测定的方法。 在每次运行中，以相应的互相关频率检测运行的信号响应的强度。 信号响应的检测在每次运行中延长，直到在每个不同的运行中已经获得具有指定的标准偏差的积分信号。 优选地，顺序运行由程序自动执行。 此外，通过导出每次运行中的结果信号响应而感测的波形被折叠。 也就是说，叠加相应的波形段以获得每个波形的平均波形值，该平均波形值相对于各个波形段具有增加的信噪比。 此外，优选地，软件创建的可变频数字滤波器用于在互相关频率处对信号响应进行滤波。