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    NUCLEIC ACID SEQUENCING WITH HYBRID NANOPORE SENSORS
    4.
    发明公开
    NUCLEIC ACID SEQUENCING WITH HYBRID NANOPORE SENSORS 审中-实审

    公开(公告)号：EP4379373A3

    公开(公告)日：2024-07-10

    申请号：EP24167857.2

    申请日：2015-07-29

    申请人： Illumina, Inc.

    发明人： BOYANOV, Boyan ,  MANDELL, Jeffrey G. ,  GUNDERSON, Kevin L. ,  BAI, Jingwei ,  QIANG, Liangliang ,  BAAS, Bradley

    IPC分类号： G01N33/487

    CPC分类号： G01N33/48721

    摘要： Provided is a system for detection of nucleic acid, the system comprising:
    (i) a detection apparatus, wherein the detection apparatus comprises one or more protein nanopores and (ii) an amplifier configured to amplify electrical signals generated at the nanopore and/or a computer or data acquisition circuit coupled to the detection apparatus to evaluate signals detected from the protein nanopore. In one aspect, the detection apparatus comprises a solid support with an array of solid-state nanopores and a plurality of lipid nanodiscs with protein nanopores, wherein the lipid nanodiscs are disposed on the solid support such that they form seals at the solid-state nanopores. In another aspect, the detection apparatus comprises one or more nanopores that are surrounded by a membrane, wherein each nanopore is tethered to an electrode. Also provided are methods of sequencing nucleic acids using the systems.

    HYBRID NANOPORE SENSORS
    7.
    发明公开
    HYBRID NANOPORE SENSORS 审中-公开
    混合纳米粒子

    公开(公告)号：EP3175237A1

    公开(公告)日：2017-06-07

    申请号：EP15748142.5

    申请日：2015-07-29

    申请人： Illumina, Inc.

    发明人： BOYANOV, Boyan ,  MANDELL, Jeffrey G. ,  GUNDERSON, Kevin L. ,  BAI, Jingwei ,  QIANG, Liangliang ,  BAAS, Bradley

    IPC分类号： G01N33/487

    摘要： The disclosure provides detection apparatus having one or more nanopores, methods for making apparatus having one or more nanopore and methods for using apparatus having one or more nanopores. Uses include, but are not limited to detection and sequencing of nucleic acids.

    摘要翻译： 在一个方面，检测装置包括具有固态纳米孔阵列的固体支持物和具有蛋白质纳米孔的多个脂质纳米圆盘，其中所述脂质纳米圆盘设置在固体支持物上，使得它们在固态纳米孔形成密封 。 另一方面，一种检测装置包括被膜包围的一个或多个纳米孔，其中每个纳米孔被束缚于电极。

    NUCLEIC ACID SEQUENCING WITH HYBRID NANOPORE SENSORS
    8.
    发明公开
    NUCLEIC ACID SEQUENCING WITH HYBRID NANOPORE SENSORS 审中-实审

    公开(公告)号：EP4379373A2

    公开(公告)日：2024-06-05

    申请号：EP24167857.2

    申请日：2015-07-29

    申请人： Illumina, Inc.

    发明人： BOYANOV, Boyan ,  MANDELL, Jeffrey G. ,  GUNDERSON, Kevin L. ,  BAI, Jingwei ,  QIANG, Liangliang ,  BAAS, Bradley

    IPC分类号： G01N33/487

    CPC分类号： G01N33/48721

    摘要： Provided is a system for detection of nucleic acid, the system comprising:
    (i) a detection apparatus, wherein the detection apparatus comprises one or more protein nanopores and (ii) an amplifier configured to amplify electrical signals generated at the nanopore and/or a computer or data acquisition circuit coupled to the detection apparatus to evaluate signals detected from the protein nanopore. In one aspect, the detection apparatus comprises a solid support with an array of solid-state nanopores and a plurality of lipid nanodiscs with protein nanopores, wherein the lipid nanodiscs are disposed on the solid support such that they form seals at the solid-state nanopores. In another aspect, the detection apparatus comprises one or more nanopores that are surrounded by a membrane, wherein each nanopore is tethered to an electrode. Also provided are methods of sequencing nucleic acids using the systems.

    HYBRID NANOPORE SENSORS
    9.
    发明公开
    HYBRID NANOPORE SENSORS 审中-公开

    公开(公告)号：EP3982121A1

    公开(公告)日：2022-04-13

    申请号：EP21212383.0

    申请日：2015-07-29

    申请人： Illumina, Inc.

    发明人： BOYANOV, Boyan ,  MANDELL, Jeffrey G. ,  GUNDERSON, Kevin L. ,  BAI, Jingwei ,  QIANG, Liangliang ,  BAAS, Bradley

    IPC分类号： G01N33/487

    摘要： The present invention provides a method of sequencing nucleic acids, comprising:
    (a) providing a detection apparatus comprising:
    (i) a solid support comprising an array of solid state nanopores, wherein the solid-state nanopores form apertures between reservoirs that are separated by the solid support;
    (ii) a plurality of lipid nanodiscs on the surface of the solid support, wherein each of the lipid nanodiscs forms a seal at each of the solid state nanopores, and wherein the lipid nanodiscs are separated from each other by interstitial regions on the surface of the solid support; and
    (iii) a plurality of protein nanopores inserted in the lipid nanodiscs to create apertures in each of the seals; and

    (b) detecting passage, through the apertures in each of the seals, of
    (i) a nucleic acid,
    (ii) a series of nucleotides removed from the nucleic acid, or
    (iii) a series of probes derived from nucleotides incorporated into the nucleic acid,

    thereby determining the sequence of the nucleic acid.

    COMPOSITIONS AND METHODS FOR SINGLE MOLECULAR PLACEMENT ON A SUBSTRATE
    10.
    发明公开
    COMPOSITIONS AND METHODS FOR SINGLE MOLECULAR PLACEMENT ON A SUBSTRATE 审中-公开

    公开(公告)号：EP3882356A1

    公开(公告)日：2021-09-22

    申请号：EP21157018.9

    申请日：2015-12-14

    申请人： Illumina, Inc.

    发明人： GUNDERSON, Kevin L. ,  BAI, Jingwei ,  BOYANOV, Boyan

    IPC分类号： C12Q1/68 ,  C12Q1/6837

    摘要： Provided herein is a substrate comprising a plurality of first and second capture primers immobilized to a feature on the substrate; at least one target polynucleotide, one end attached to one of said capture primers and the other end linked to a target molecule, wherein said target polynucleotide comprises a target region flanked by first and second capture primer binding regions complementary to said first and second capture primers, said second capture primer binding region comprising a base pair mismatch to said second capture primer, and a plurality of clonal amplicons complementary to said target polynucleotide immobilized to said feature. Provided is a method for placing single target molecules on the substrate.