公开(公告)号：EP4379373A3

公开(公告)日：2024-07-10

申请号：EP24167857.2

申请日：2015-07-29

申请人： Illumina, Inc.

发明人： BOYANOV, Boyan ,  MANDELL, Jeffrey G. ,  GUNDERSON, Kevin L. ,  BAI, Jingwei ,  QIANG, Liangliang ,  BAAS, Bradley

IPC分类号： G01N33/487

CPC分类号： G01N33/48721

摘要： Provided is a system for detection of nucleic acid, the system comprising:
(i) a detection apparatus, wherein the detection apparatus comprises one or more protein nanopores and (ii) an amplifier configured to amplify electrical signals generated at the nanopore and/or a computer or data acquisition circuit coupled to the detection apparatus to evaluate signals detected from the protein nanopore. In one aspect, the detection apparatus comprises a solid support with an array of solid-state nanopores and a plurality of lipid nanodiscs with protein nanopores, wherein the lipid nanodiscs are disposed on the solid support such that they form seals at the solid-state nanopores. In another aspect, the detection apparatus comprises one or more nanopores that are surrounded by a membrane, wherein each nanopore is tethered to an electrode. Also provided are methods of sequencing nucleic acids using the systems.

公开(公告)号：EP3725893A1

公开(公告)日：2020-10-21

申请号：EP20165203.9

申请日：2016-02-10

申请人： Illumina, Inc.

发明人： FISHER, Jeffrey S. ,  GUNDERSON, Kevin L. ,  RIGATTI, Roberto ,  STEEMERS, Frank J.

IPC分类号： C12Q1/6806

摘要： Embodiments of the present invention relate to analyzing components of a cell. In some embodiments, the present invention relate to analyzing components of a single cell. In some embodiments, the methods and compositions relate to sequencing nucleic acids. In some embodiments, the methods and compositions relate to identifying and/or quantitating nucleic acid, proteins, organelles, and/or cellular metabolites.

公开(公告)号：EP3305400A3

公开(公告)日：2018-06-06

申请号：EP17185952.3

申请日：2012-10-26

申请人： Illumina, Inc.

发明人： BOWEN, Shane M. ,  GUNDERSON, Kevin L. ,  LIN, Shengrong ,  BACIGALUPO, Maria Candelaria Rogert ,  VIJAYAN,, Kandaswamy ,  WU, Yir-Shyuan ,  VENKATESAN, Bala Murali ,  TSAY, James ,  BEIERLE, John M. ,  BERTI, Lorenzo ,  PARK, Sang Ryul

IPC分类号： B01J19/00 ,  C12Q1/68 ,  G01N33/543 ,  C40B50/18

CPC分类号： C12Q1/6844 ,  B01J19/0046 ,  B01J2219/00529 ,  B01J2219/00608 ,  B01J2219/00621 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00644 ,  B01J2219/00648 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00662 ,  B01J2219/00722 ,  B01J2219/00788 ,  C12Q1/686 ,  C40B50/18 ,  C12Q2527/125 ,  C12Q2535/122 ,  C12Q2565/543

摘要： A microarray is designed capture one or more molecules of interest at each of a plurality of sites on a substrate. The sites comprise base pads, such as polymer base pads, that promote the attachment of the molecules at the sites. The microarray may be made by one or more patterning techniques to create a layout of base pads in a desired pattern. Further, the microarrays may include features to encourage clonality at the 14 sites.

公开(公告)号：EP2771103A2

公开(公告)日：2014-09-03

申请号：EP12791335.8

申请日：2012-10-26

申请人： Illumina, Inc.

发明人： BOWEN, Shane M. ,  GUNDERSON, Kevin L. ,  LIN, Shengrong ,  BACIGALUPO, Maria Candelaria Rogert ,  VIJAYAN, Kandaswamy ,  WU, Yir-Shyuan ,  VENKATESAN, Bala Murali ,  TSAY, James ,  BEIERLE, John M. ,  BERTI, Lorenzo ,  PARK, Sang Ryul

IPC分类号： B01J19/00 ,  C12Q1/68 ,  G01N33/543

CPC分类号： C12Q1/6844 ,  B01J19/0046 ,  B01J2219/00529 ,  B01J2219/00608 ,  B01J2219/00621 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00644 ,  B01J2219/00648 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00662 ,  B01J2219/00722 ,  B01J2219/00788 ,  C12Q1/686 ,  C40B50/18 ,  C12Q2527/125 ,  C12Q2535/122 ,  C12Q2565/543

摘要： A microarray is designed capture one or more molecules of interest at each of a plurality of sites on a substrate. The sites comprise base pads, such as polymer base pads, that promote the attachment of the molecules at the sites. The microarray may be made by one or more patterning techniques to create a layout of base pads in a desired pattern. Further, the microarrays may include features to encourage clonality at the sites.

摘要翻译： 设计的微阵列在基底上的多个位点的每一个处捕获一个或多个感兴趣的分子。 这些部位包括促进分子在部位附着的基底垫，例如聚合物基底垫。 微阵列可以通过一种或多种图案化技术制成，以产生所需图案的基底布局。 此外，微阵列可以包括在站点鼓励克隆性的特征。

公开(公告)号：EP4071253A1

公开(公告)日：2022-10-12

申请号：EP22163380.3

申请日：2013-02-21

申请人： Illumina, Inc.

发明人： FAN, Jian-Bing ,  GUNDERSON, Kevin L. ,  STEEMERS, Frank J. ,  POKHOLOK, Dmitry K. ,  KAPER, Fiona

IPC分类号： C12Q1/6844

摘要： The present disclosure relates to systems and methods for the amplification of nucleic acids, including, but not limited to, the amplification of nucleic acid libraries and whole genome amplification.

公开(公告)号：EP3828279A1

公开(公告)日：2021-06-02

申请号：EP20205271.8

申请日：2015-07-14

申请人： Illumina, Inc.

发明人： BAI, Jingwei ,  BOYANOV, Boyan ,  CHEN, Cheng-Yao ,  GUNDERSON, Kevin L. ,  MANDELL, Jeffrey G. ,  PERBOST, Michel

IPC分类号： C12Q1/68

摘要： A method of nucleic acid sequencing. The method can include the steps of (a) providing a polymerase tethered to a solid support charge sensor; (b) providing one or more nucleotides, whereby the presence of the nucleotide can be detected by the charge sensor; and (c) detecting incorporation of the nucleotide into a nascent strand complementary to a template nucleic acid.

公开(公告)号：EP3682020A1

公开(公告)日：2020-07-22

申请号：EP18855326.7

申请日：2018-09-14

申请人： Illumina, Inc.

发明人： MANDELL, Jeffrey G. ,  GUNDERSON, Kevin L. ,  KEEHAN, Michael Gregory ,  GARCIA, Erin Christine ,  GUNDLACH, Jens H.

IPC分类号： C12Q1/48 ,  C12Q1/68 ,  G01N27/00 ,  G01N30/02 ,  B05D5/12

公开(公告)号：EP3256604B1

公开(公告)日：2020-03-25

申请号：EP16711039.4

申请日：2016-02-10

申请人： Illumina, Inc.

发明人： GUNDERSON, Kevin L. ,  STEEMERS, Frank J. ,  FISHER, Jeffrey S. ,  RIGATTI, Roberto

IPC分类号： C12Q1/6806 ,  C12Q1/6869 ,  C12N15/10

公开(公告)号：EP3305400A2

公开(公告)日：2018-04-11

申请号：EP17185952.3

申请日：2012-10-26

申请人： Illumina, Inc.

发明人： BOWEN, Shane M. ,  GUNDERSON, Kevin L. ,  LIN, Shengrong ,  BACIGALUPO, Maria Candelaria Rogert ,  VIJAYAN,, Kandaswamy ,  WU, Yir-Shyuan ,  VENKATESAN, Bala Murali ,  TSAY, James ,  BEIERLE, John M. ,  BERTI, Lorenzo ,  PARK, Sang Ryul

IPC分类号： B01J19/00 ,  C12Q1/68 ,  G01N33/543 ,  C40B50/18

CPC分类号： C12Q1/6844 ,  B01J19/0046 ,  B01J2219/00529 ,  B01J2219/00608 ,  B01J2219/00621 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00644 ,  B01J2219/00648 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00662 ,  B01J2219/00722 ,  B01J2219/00788 ,  C12Q1/686 ,  C40B50/18 ,  C12Q2527/125 ,  C12Q2535/122 ,  C12Q2565/543

摘要： A microarray is designed capture one or more molecules of interest at each of a plurality of sites on a substrate. The sites comprise base pads, such as polymer base pads, that promote the attachment of the molecules at the sites. The microarray may be made by one or more patterning techniques to create a layout of base pads in a desired pattern. Further, the microarrays may include features to encourage clonality at the 14 sites.

摘要翻译： 微阵列被设计为在基底上的多个位点中的每一个处捕获一个或多个感兴趣的分子。 这些位点包含基垫，例如聚合物基垫，其促进分子在该位点处的附着。 微阵列可以通过一种或多种图案化技术制成，以便以期望的图案形成基垫的布局。 此外，微阵列可能包括鼓励在14个地点克隆的特征。

公开(公告)号：EP3175237B1

公开(公告)日：2020-03-04

申请号：EP15748142.5

申请日：2015-07-29

申请人： Illumina, Inc.

发明人： BOYANOV, Boyan ,  MANDELL, Jeffrey G. ,  GUNDERSON, Kevin L. ,  BAI, Jingwei ,  QIANG, Liangliang ,  BAAS, Bradley

IPC分类号： G01N33/487