公开(公告)号：EP3202909A1

公开(公告)日：2017-08-09

申请号：EP15847264.7

申请日：2015-07-23

申请人： Industry-Academic Cooperation Foundation Dankook University

发明人： LEE, Seong Wook ,  KIM, Ju Hyun ,  JEONG, Jin Sook ,  HAN, Sang Young

IPC分类号： C12N15/85 ,  C12N9/22 ,  A61K31/713 ,  A61K38/43 ,  A61P35/00

CPC分类号： C12N15/113 ,  A61K31/713 ,  A61K38/00 ,  A61K38/43 ,  A61K48/0058 ,  A61K48/0066 ,  C07K14/4705 ,  C07K2319/01 ,  C07K2319/33 ,  C07K2319/61 ,  C12N9/22 ,  C12N15/1135 ,  C12N15/63 ,  C12N15/79 ,  C12N15/85 ,  C12N15/86 ,  C12N2310/113 ,  C12N2310/12 ,  C12N2310/124 ,  C12N2310/128 ,  C12N2330/51 ,  C12N2710/10343 ,  C12N2830/008 ,  C12N2830/42 ,  C12N2830/48

摘要： The present invention relates to a recombination vector, a transformation cell into which the recombinant vector is introduced, a ribozyme expressed from the recombination vector, a prophylactic or therapeutic composition for liver cancer comprising the recombination vector and the ribozyme, and a therapeutic method for liver cancer using the composition, said recombination vector comprising: a tissue-specific promoter; and a ribozyme-target gene expression cassette comprising a trans-splicing ribozyme targeting a cancer-specific gene and a target gene connected to the 3' exon of the ribozyme, wherein a splicing donor/splicing acceptor sequence (SD/SA sequence) is connected to the 5' end of the ribozyme-target gene expression cassette, woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) is connected to the 3' end of the ribozyme-target gene expression cassette, and a nucleic acid sequence recognizing a micro RNA-122a (microRNA-122a, miR-122a) is further connected to the 3' end of the WPRE.

摘要翻译： 本发明涉及重组载体，导入重组载体的转化细胞，由该重组载体表达的核酶，含有重组载体和核酶的肝癌的预防或治疗组合物，以及肝的治疗方法 使用所述组合物的癌症，所述重组载体包含：组织特异性启动子; 和包含靶向癌症特异性基因的转拼核酶和连接于核酶的3'外显子的靶基因的核酶靶基因表达盒，其中连接剪接供体/剪接受体序列（SD / SA序列） 到核酶靶基因表达盒的5'末端，将土拨鼠肝炎病毒转录后调节元件（WPRE）连接到核酶靶基因表达盒的3'末端，并且将识别微小RNA-122a的核酸序列 （microRNA-122a，miR-122a）进一步连接到WPRE的3'末端。