公开(公告)号：EP2048954B1

公开(公告)日：2012-03-28

申请号：EP07776311.8

申请日：2007-04-26

申请人： Inis Biotech LLC ,  Inis Biotech SA

发明人： PODHAJCER, Osvaldo, Luis ,  CAFFERATA, Eduardo, Gustavo, Alfredo ,  LOPEZ, Maria, Veronica ,  VIALE, Diego, Luis

IPC分类号： A01N63/00 ,  A61K39/12

CPC分类号： C07K14/4748

摘要： An isolated DNA sequence corresponding to a region of the SPARC gene human promoter from base pair -513 to base pair +3 5 capable of driving the expression of a heterologous gene of interest, that can be associated to any other promoter sequence, such as radiation responsive sequence, hypoxia responsive sequence and free-radical responsive sequence. The invention also provides constructs and DNA recombinant expression viral vectors, comprising said isolated sequence of the SPARC gene human promoter and at least one heterologous gene operably linked thereto, wherein said promoter sequence drives the expression of the at least one heterologous gene in tumor cells. Pharmaceutical compositions and a method for treating tumors are also provided.

公开(公告)号：EP2048954A2

公开(公告)日：2009-04-22

申请号：EP07776311.8

申请日：2007-04-26

申请人： Inis Biotech LLC

发明人： PODHAJCER, Osvaldo, Luis ,  CAFFERATA, Eduardo, Gustavo, Alfredo ,  LOPEZ, Maria, Veronica ,  VIALE, Diego, Luis

IPC分类号： A01N63/00 ,  A61K39/12

CPC分类号： C07K14/4748

摘要： An isolated DNA sequence corresponding to a region of the SPARC gene human promoter from base pair -513 to base pair +3 5 capable of driving the expression of a heterologous gene of interest, that can be associated to any other promoter sequence, such as radiation responsive sequence, hypoxia responsive sequence and free-radical responsive sequence. The invention also provides constructs and DNA recombinant expression viral vectors, comprising said isolated sequence of the SPARC gene human promoter and at least one heterologous gene operably linked thereto, wherein said promoter sequence drives the expression of the at least one heterologous gene in tumor cells. Pharmaceutical compositions and a method for treating tumors are also provided.

公开(公告)号：EP2021034A2

公开(公告)日：2009-02-11

申请号：EP07761397.4

申请日：2007-04-26

申请人： Inis Biotech LLC

发明人： PODHAJCER, Osvaldo, Luis ,  CAFFERATA, Eduardo, Gustavo, Alfredo ,  LOPEZ, Maria, Veronica ,  MACCIO, Daniela, Rita ,  VIALE, Diego, Luis

IPC分类号： A61K48/00 ,  C12N15/85 ,  C07H21/04

CPC分类号： A61K31/555 ,  A61K31/513 ,  A61K31/519 ,  A61K31/7068 ,  A61K35/761 ,  A61K38/00 ,  A61K38/162 ,  A61K45/06 ,  C12N7/00 ,  C12N15/85 ,  C12N15/86 ,  C12N2710/10332 ,  C12N2710/10343 ,  C12N2830/002 ,  C12N2830/85 ,  A61K2300/00

摘要： An isolated DNA sequence that corresponds to a region of the human A33 gene promoter from base pair −105 to base pair +307, able to control the expression of a heterologous gene of interest, that may be used in conjunction with any other regulatory sequence, including sequences responsive to stress such as radiation, hypoxia and free radical formation. Constructs and viral vectors for recombinant DNA expression are provided, which include this sequence of human A33 gene promoter and, operably linked thereto, at least one heterologous gene. The regulatory sequence(s) control(s) the expression of at least one heterologous gene in cells including colorectal cancer cells. Pharmaceutical compositions and methods for treating colorectal cancer are also described.