公开(公告)号：EP1461080A2

公开(公告)日：2004-09-29

申请号：EP02796675.3

申请日：2002-12-18

申请人： INNOGENETICS N.V.

发明人： MAERTENS, Geert ,  DEPLA, Erik ,  BOSMAN, Fons

IPC分类号： A61K39/29

CPC分类号： C07K14/005 ,  A61K38/00 ,  A61K39/00 ,  C12N2770/24222

摘要： The present invention relates to a method for purifying recombinant HCV single or specific oligomeric envelope proteins selected from the group consisting of E1 and/or E2 and/or E1/E2, characterized in that upon lysing the transformed host cells to isolate the recombinantly expressed protein a disulphide bond cleavage or reduction step is carried out with a disulphide bond cleavage agent. The present invention also relates to a composition isolated by such a method. The present invention also relates to the diagnostic and therapeutic application of these compositions. Furthermore, the invention relates to the use of HCV E1 protein and peptides for prognosing and monitoring the clinical effectiveness and/or clinical outcome of HCV treatment.

公开(公告)号：EP0721505B1

公开(公告)日：2002-05-08

申请号：EP95930434.6

申请日：1995-07-31

申请人： INNOGENETICS N.V.

发明人： MAERTENS, Geert ,  BOSMAN, Fons ,  DE MARTYNOFF, Guy ,  BUYSE, Marie-Ange

IPC分类号： C12N15/40 ,  C07K14/18 ,  C07K16/10 ,  C12Q1/70 ,  G01N33/569

CPC分类号： C07K16/109 ,  A61K39/00 ,  A61K39/12 ,  A61K39/29 ,  A61K2039/5256 ,  C07K14/005 ,  C07K2317/34 ,  C12N2710/24143 ,  C12N2770/24222 ,  C12N2770/24234 ,  C12N2770/24243 ,  Y10S435/803 ,  Y10S435/81 ,  Y10S435/915 ,  Y10S435/975

公开(公告)号：EP0721505B2

公开(公告)日：2006-05-10

申请号：EP95930434.6

申请日：1995-07-31

申请人： Innogenetics N.V.

发明人： MAERTENS, Geert ,  BOSMAN, Fons ,  DE MARTYNOFF, Guy ,  BUYSE, Marie-Ange

IPC分类号： C12N15/40 ,  C07K14/18 ,  C07K16/10 ,  C12Q1/70 ,  G01N33/569

CPC分类号： C07K16/109 ,  A61K39/00 ,  A61K39/12 ,  A61K39/29 ,  A61K2039/5256 ,  C07K14/005 ,  C07K2317/34 ,  C12N2710/24143 ,  C12N2770/24222 ,  C12N2770/24234 ,  C12N2770/24243 ,  Y10S435/803 ,  Y10S435/81 ,  Y10S435/915 ,  Y10S435/975

摘要： The present invention relates to a method for purifying recombinant HCV single or specific oligomeric envelope proteins selected from the group consisting of E1 and/or E2 and/or E1/E2, characterized in that upon lysing the transformed host cells to isolate the recombinantly expressed protein a disulphide bond cleavage or reduction step is carried out with a disulphide bond cleavage agent. The present invention also relates to a composition isolated by such a method. The present invention also relates to the diagnostic and therapeutic application of these compositions. Furthermore, the invention relates to the use of HCV E1 protein and peptides for prognosing and monitoring the clinical effectiveness and/or clinical outcome of HCV treatment.

公开(公告)号：EP1463753A2

公开(公告)日：2004-10-06

申请号：EP02704649.9

申请日：2002-01-11

申请人： INNOGENETICS N.V.

发明人： MAERTENS, Geert ,  BOSMAN, Fons ,  DEPLA, Erik

IPC分类号： C07K14/18 ,  A61K39/29 ,  G01N33/576 ,  C12Q1/70 ,  C12N15/40

CPC分类号： C07K14/005 ,  A61K39/00 ,  C12N2710/24143 ,  C12N2770/24222

摘要： The present invention relates to a method for purifying recombinant HCV single or specific oligomeric envelope proteins selected from the group consisting of E1 and/or E2 and/or E1/E2, characterized in that upon lysing the transformed host cells to isolate the recombinantly expressed protein a disulphide bond cleavage or reduction step is carried out with a disulphide bond cleavage agent. The present invention also relates to a composition isolated by such a method. The present invention also relates to the diagnostic ad therapeutic application of these compositions. Furthermore, the invention relates to the use of HCV E1 protein and peptides for prognosing and monitoring the clinical effectiveness and/or clinical outcome if HCV treatment.

公开(公告)号：EP0721505A1

公开(公告)日：1996-07-17

申请号：EP95930434.0

申请日：1995-07-31

申请人： INNOGENETICS N.V.

发明人： MAERTENS, Geert ,  BOSMAN, Fons ,  DE MARTYNOFF, Guy ,  BUYSE, Marie-Ange

IPC分类号： G01N33 ,  A61K38 ,  A61K39 ,  A61P1 ,  A61P31 ,  C07K14 ,  C07K16 ,  C12N1 ,  C12N5 ,  C12N15 ,  C12P21 ,  C12Q1 ,  C12R1

CPC分类号： C07K16/109 ,  A61K39/00 ,  A61K39/12 ,  A61K39/29 ,  A61K2039/5256 ,  C07K14/005 ,  C07K2317/34 ,  C12N2710/24143 ,  C12N2770/24222 ,  C12N2770/24234 ,  C12N2770/24243 ,  Y10S435/803 ,  Y10S435/81 ,  Y10S435/915 ,  Y10S435/975

摘要： The present invention relates to a method for purifying recombinant HCV single or specific oligomeric envelope proteins selected from the group consisting of E1 and/or E2 and/or E1/E2, characterized in that upon lysing the transformed host cells to isolate the recombinantly expressed protein a disulphide bond cleavage or reduction step is carried out with a disulphide bond cleavage agent. The present invention also relates to a composition isolated by such a method. The present invention also relates to the diagnostic and therapeutic application of these compositions. Furthermore, the invention relates to the use of HCV E1 protein and peptides for prognosing and monitoring the clinical effectiveness and/or clinical outcome of HCV treatment.