公开(公告)号：EP2591336B1

公开(公告)日：2017-05-17

申请号：EP11743307.8

申请日：2011-07-06

申请人： Institut National de la Recherche Agronomique (INRA)

发明人： CATHALA, Bernard ,  CERCLIER, Carole

IPC分类号： G01N21/45 ,  G01N21/78 ,  G01N21/77 ,  C12Q1/34

CPC分类号： C12Q1/34 ,  G01N21/45 ,  G01N21/78 ,  G01N2021/7779

公开(公告)号：EP2591336A1

公开(公告)日：2013-05-15

申请号：EP11743307.8

申请日：2011-07-06

申请人： Institut National De La Recherche Agronomique (INRA)

发明人： CATHALA, Bernard ,  CERCLIER, Carole

IPC分类号： G01N21/25 ,  G01N21/45 ,  G01N21/77 ,  G01N33/52

CPC分类号： C12Q1/34 ,  G01N21/45 ,  G01N21/78 ,  G01N2021/7779

摘要： The present invention relates to a colorimetric device for detecting, in an aqueous solution of interest, hydrolytic enzymatic activity with regard to at least one polymer of interest. This device (1) comprises (i) a substrate (2) and (ii) a transparent detection layer (3) that includes the polymer of interest. The detection layer (3) is adapted to: maintain a first thickness
e after application of the aqueous solution of interest when the latter has no hydrolytic enzymatic activity; and have a second thickness
e' , smaller than the first thickness
e , after application of the aqueous solution of interest when the latter has hydrolytic enzymatic activity. This first thickness
e and/or the second thickness
e' of the transparent detection layer (3) are/is designed to generate a colour by means of an optical interference effect produced by the recombining of the light beams reflected at the interfaces (4, 5) of said detection layer (3).