公开(公告)号：EP2614149B1

公开(公告)日：2015-04-08

申请号：EP11760620.2

申请日：2011-09-07

申请人： Integrated Dna Technologies, Inc.

发明人： BEHLKE, Mark, Aaron ,  OWCZARZY, Richard ,  YOU, Yong ,  WALDER, Joseph, Alan ,  LENNOX, Kim

IPC分类号： C12N15/113 ,  C12N15/11

CPC分类号： C12N15/113 ,  C12N15/111 ,  C12N2310/11 ,  C12N2310/141 ,  C12N2310/315 ,  C12N2310/321 ,  C12N2310/344 ,  C12N2310/352 ,  C12N2310/3529 ,  C12N2320/51 ,  C12Q1/6876

公开(公告)号：EP2614149A1

公开(公告)日：2013-07-17

申请号：EP11760620.2

申请日：2011-09-07

申请人： Integrated Dna Technologies, Inc.

发明人： BEHLKE, Mark, Aaron ,  OWCZARZY, Richard ,  YOU, Yong ,  WALDER, Joseph, Alan ,  LENNOX, Kim

IPC分类号： C12N15/113

CPC分类号： C12N15/113 ,  C12N15/111 ,  C12N2310/11 ,  C12N2310/141 ,  C12N2310/315 ,  C12N2310/321 ,  C12N2310/344 ,  C12N2310/352 ,  C12N2310/3529 ,  C12N2320/51 ,  C12Q1/6876

摘要： The invention pertains to modifications for antisense oligonucleotides, wherein the modifications are used to improve stability and provide protection from nuclease degradation. The modifications could also be incorporated into double-stranded nucleic acids, such as synthetic siRNAs and miRNAs.

公开(公告)号：EP2118651A2

公开(公告)日：2009-11-18

申请号：EP08705755.0

申请日：2008-01-07

申请人： Integrated Dna Technologies, Inc.

发明人： OWCZARZY, Richard ,  MOREIRA, Bernardo ,  YOU, Yong ,  BEHLKE, Mark Aaron ,  WALDER, Joseph Alan

IPC分类号： G01N33/48

CPC分类号： G06F19/20 ,  G06F19/18

摘要： The invention relates to methods and systems for predicting or estimating the melting temperature of duplex nucleic acids, in the presence of divalent cations, particularly duplexes of oligonucleotides which may be used as, for example, but not limited to primers or probes in PCR and/or hybridization assays. The methods and algorithms use novel formulas, having terms and coefficients that are functions of the particular nucleotide sequence, to estimate the effect of divalent cation salt conditions on the melting temperature.