公开(公告)号：EP2441831B1

公开(公告)日：2018-08-22

申请号：EP10786229.4

申请日：2010-06-10

申请人： Inter-University Research Institute Corporation Research Organization of Information and Systems ,  Kyowa Hakko Kirin Co., Ltd.

发明人： KAWAKAMI, Koichi ,  YAMAGUCHI, Keina ,  OGAWA, Risa ,  TSUKAHARA, Masayoshi

IPC分类号： C12N15/00 ,  C12N5/10 ,  C12N15/09 ,  C12P21/02 ,  C12P21/08 ,  C12N15/85

CPC分类号： C12P21/00 ,  C07K16/1018 ,  C12N15/85 ,  C12N2015/8518 ,  C12N2800/107 ,  C12N2800/90 ,  C12N2840/20 ,  C12P21/02

摘要： This invention relates to a method for producing a protein of interest, comprising introducing a protein expression vector which comprises a gene fragment a gene fragment comprising a DNA encoding a protein of interest and a selectable marker gene and transposon sequences at both terminals of the gene fragment, into a suspension mammalian cell; integrating the gene fragment inserted between a pair of the transposon sequences, into a chromosome of the mammalian cell to obtain a mammalian cell capable of expressing the protein of interest; and suspension-culturing the mammalian cell; and a suspension mammalian cell capable of expressing the protein of interest.

公开(公告)号：EP2441831B9

公开(公告)日：2019-03-06

申请号：EP10786229.4

申请日：2010-06-10

申请人： Inter-University Research Institute Corporation Research Organization of Information and Systems ,  Kyowa Hakko Kirin Co., Ltd.

发明人： KAWAKAMI, Koichi ,  YAMAGUCHI, Keina ,  OGAWA, Risa ,  TSUKAHARA, Masayoshi

IPC分类号： C12N15/00 ,  C12N5/10 ,  C12N15/09 ,  C12P21/02 ,  C12P21/08 ,  C12N15/85

公开(公告)号：EP2441831A1

公开(公告)日：2012-04-18

申请号：EP10786229.4

申请日：2010-06-10

申请人： Inter-University Research Institute Corporation Research Organization of Information and Systems ,  Kyowa Hakko Kirin Co., Ltd.

发明人： KAWAKAMI, Koichi ,  YAMAGUCHI, Keina ,  OGAWA, Risa ,  TSUKAHARA, Masayoshi

IPC分类号： C12N15/00 ,  C12N5/10 ,  C12N15/09 ,  C12P21/02 ,  C12P21/08

CPC分类号： C12P21/00 ,  C07K16/1018 ,  C12N15/85 ,  C12N2015/8518 ,  C12N2800/107 ,  C12N2800/90 ,  C12N2840/20 ,  C12P21/02

摘要： This invention relates to a method for producing a protein of interest, comprising introducing a protein expression vector which comprises a gene fragment a gene fragment comprising a DNA encoding a protein of interest and a selectable marker gene and transposon sequences at both terminals of the gene fragment, into a suspension mammalian cell; integrating the gene fragment inserted between a pair of the transposon sequences, into a chromosome of the mammalian cell to obtain a mammalian cell capable of expressing the protein of interest; and suspension-culturing the mammalian cell; and a suspension mammalian cell capable of expressing the protein of interest.

摘要翻译： 本发明涉及一种生产目的蛋白质的方法，包括引入蛋白质表达载体，该载体包含基因片段，该基因片段包含编码目标蛋白的DNA和选择标记基因的基因片段以及基因片段两端的转座子序列 ，进入悬浮哺乳动物细胞; 将插入在一对转座子序列之间的基因片段整合到哺乳动物细胞的染色体中以获得能够表达目的蛋白质的哺乳动物细胞; 并悬浮培养哺乳动物细胞; 和能够表达目的蛋白质的悬浮哺乳动物细胞。

公开(公告)号：EP3564367A1

公开(公告)日：2019-11-06

申请号：EP17886849.3

申请日：2017-12-27

申请人： Kyowa Hakko Kirin Co., Ltd.

发明人： YAMAMOTO, Koichi ,  MATSUMOTO, Yuichi ,  YAMAGUCHI, Keina ,  FUJII, Hiroto ,  SUZAWA, Toshiyuki

IPC分类号： C12N15/09 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12P21/00 ,  C12P21/08 ,  C12Q1/04 ,  C12Q1/68

摘要： The purpose of the present invention is to provide a method of selecting cell lines in which the reduction of a recombinant protein has been suppressed. The present invention pertains to a method of selecting cells, said method comprising a first step in which the intracellular expression level of at least one gene selected from among genes having any one of the base sequences represented by SEQ ID NO: 1-16 and orthologs thereof is measured, and a second step in which the expression level achieved by suppressing the reduction of a recombinant protein is evaluated on the basis of the difference determined by comparing the gene expression level measured in the first step and a control value for the gene expression level.