公开(公告)号：EP3320773A1

公开(公告)日：2018-05-16

申请号：EP16824403.6

申请日：2016-07-08

申请人： Chugai Seiyaku Kabushiki Kaisha

发明人： JISHAGE, Koichi ,  UEDA, Otoya ,  WADA, Naoko ,  ISHIGURO, Takahiro ,  KINOSHITA, Yasuko

IPC分类号： A01K67/027 ,  G01N33/15 ,  G01N33/50 ,  C12N15/09

CPC分类号： A01K67/0278 ,  A01K2207/12 ,  A01K2217/072 ,  A01K2227/105 ,  A01K2267/0325 ,  A01K2267/0331 ,  C07K14/7051 ,  C07K16/2809 ,  C12N15/09 ,  C12N15/8509 ,  C12N2015/8518 ,  G01N33/15 ,  G01N33/5008

摘要： The present invention provides genetically modified non-human animals which are deficient in at least one or more types of CD3 genes selected from the group consisting of endogenous CD3ε, CD3δ, and CD3γ in its genome and functionally express at least one or more types of human CD3 genes selected from the group consisting of human CD3ε, CD3δ, and CD3γ. In the genetically modified non-human animals of the present invention, mature T cell differentiation and production can take place, and immunocompetent cells including T cells can exert their functions. The genetically modified non-human animals of the present invention enable efficient evaluation and screening in the development of therapeutic agents and therapeutic methods that use human CD3-mediated targeted drugs.

摘要翻译： 本发明提供遗传修饰的非人动物，其缺失至少一种或多种类型的CD3基因，所述基因选自由其基因组中的内源性CD3ε，CD3δ和CD3γ组成的组，并且其功能性表达至少一种或多种类型的人 选自人CD3ε，CD3δ和CD3γ的CD3基因。 在本发明的基因修饰的非人动物中，可以发生成熟的T细胞分化和产生，并且包括T细胞的免疫活性细胞可以发挥其功能。 本发明的基因修饰的非人动物能够有效评估和筛选开发使用人CD3介导的靶向药物的治疗剂和治疗方法。

公开(公告)号：EP3313175A1

公开(公告)日：2018-05-02

申请号：EP16736963.6

申请日：2016-06-22

申请人： The Jackson Laboratory

发明人： KECK, James

IPC分类号： A01K67/027

CPC分类号： C12N15/8509 ,  A01K67/0271 ,  A01K67/0278 ,  A01K2207/12 ,  A01K2207/15 ,  A01K2217/075 ,  A01K2227/105 ,  A01K2267/0331 ,  A01K2267/0362 ,  A01K2267/0387 ,  C07K14/55 ,  C07K14/70596 ,  C12N5/0663 ,  C12N2015/8518

摘要： The invention described herein provides non-HLA matched humanized mouse model (e.g., NSG mouse model) with patient-derived xenograft (PDX), as well as methods of making and using the same.

公开(公告)号：EP3285798A2

公开(公告)日：2018-02-28

申请号：EP16783724

申请日：2016-04-20

申请人： SALK INST FOR BIOLOGICAL STUDI ,  THE FLORIDA STATE UNIV RESEARCH FOUNDATION INCORPORATED

发明人： EVANS RONALD M ,  DOWNES MICHAEL ,  ATKINS ANNETTE ,  YU RUTH T ,  BLABER MICHAEL ,  XIA XUE

IPC分类号： A61K38/18 ,  C07K14/50

CPC分类号： C07K14/503 ,  A61K38/00 ,  A61K38/1825 ,  A61K38/28 ,  A61K45/06 ,  A61K47/6425 ,  A61K48/00 ,  C07K14/50 ,  C07K14/501 ,  C07K14/705 ,  C07K2319/00 ,  C07K2319/70 ,  C07K2319/75 ,  C12N15/00 ,  C12N2015/8518 ,  C12Q1/54 ,  A61K2300/00

摘要： The present disclosure provides FGF1 mutant proteins having one or more mutations in the heparin binding domain. Such mutants may also have an N-terminal deletion, point mutation(s), or combinations thereof. In some examples, the mutant FGF1 proteins have reduced mitogenic activity. Also provided are nucleic acid molecules that encode such proteins, and vectors and cells that include such nucleic acids. The disclosed FGF1 mutants can reduce blood glucose in a mammal, and in some examples are used to treat a metabolic disorder.

公开(公告)号：EP2602323B1

公开(公告)日：2018-02-28

申请号：EP12187787.2

申请日：2008-05-30

申请人： Open Monoclonal Technology, Inc.

发明人： Buelow, Ronald

IPC分类号： C12N15/13 ,  A01K67/027 ,  C07K16/00

CPC分类号： C07K16/00 ,  A01K67/0276 ,  A01K67/0278 ,  A01K2207/15 ,  A01K2217/072 ,  A01K2217/075 ,  A01K2267/01 ,  C07K2317/14 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/51 ,  C07K2317/76 ,  C12N5/0609 ,  C12N9/22 ,  C12N15/8509 ,  C12N2015/8518 ,  C12N2510/00

摘要： The invention relates to transgenic animals lacking endogenous lg and capable of producing transgenic antibodies, as well as methods of making the same. The invention further relates to methods for producing transgenic antibodies in such animals, and transgenic antibodies so produced.

公开(公告)号：EP2649178B8

公开(公告)日：2017-08-30

申请号：EP11791602.3

申请日：2011-12-09

申请人： Institut Pasteur

发明人： DESPRES, Philippe ,  PAULOUS, Sylvie ,  CRUBLET, Elodie

IPC分类号： C12N9/10 ,  C12N15/62

CPC分类号： C12N15/625 ,  C07K2319/00 ,  C07K2319/055 ,  C07K2319/20 ,  C07K2319/21 ,  C07K2319/50 ,  C12N5/0601 ,  C12N5/0693 ,  C12N9/1007 ,  C12N9/96 ,  C12N15/85 ,  C12N2015/8518 ,  C12N2510/02 ,  C12P21/02 ,  C12Y201/01063 ,  Y02A50/51 ,  Y02A50/53 ,  Y02A50/60

摘要： The present invention relates to a novel enhancer of protein production in host cells. It discloses a vector for expressing recombinant proteins in these cells, comprising a nucleotide sequence encoding a) a secretion peptidic signal, b) a 6-methylguanine-DNA-methyltransferase enzyme (MGMT, EC 2.1.1.63), a mutant or a catalytic domain thereof, and c) a recombinant protein. Said MGMT enzyme is preferably the so-called SNAP protein.

公开(公告)号：EP3086636A2

公开(公告)日：2016-11-02

申请号：EP14873362.9

申请日：2014-12-19

申请人： LFB USA, Inc.

发明人： MEADE, Harry M. ,  GAVIN, William G.

IPC分类号： A01K67/027 ,  C12P21/06 ,  C12P19/26 ,  C12N5/00

CPC分类号： C12N15/8509 ,  A01K67/0275 ,  A01K2217/15 ,  A01K2227/101 ,  A01K2227/102 ,  A01K2267/02 ,  A61K31/727 ,  C12N2015/8518 ,  C12P19/26

摘要： In one aspect, the disclosure provides methods, cells and transgenic non human mammals for the production of heparin, the cells and transgenic non human mammals thereof as well as the heparin obtained from these cells or transgenic non human mammals.

摘要翻译： 本公开提供了用于生产肝素的方法，细胞和转基因非人哺乳动物。 具体地说，该方法包括提供转基因非人哺乳动物或修饰以表达一种或多种肝素生物合成酶的乳腺上皮细胞，以及收获产生的肝素。 还提供了细胞和其转基因非人哺乳动物以及从这些细胞或转基因非人哺乳动物获得的肝素。

公开(公告)号：EP2380435B1

公开(公告)日：2016-05-25

申请号：EP11167639.1

申请日：2006-08-30

申请人： KANEKA CORPORATION

发明人： Nakaishi, Tomoyuki ,  Shindo, Takuya ,  Awa, Tomoko

IPC分类号： A01K67/027 ,  A61K38/18 ,  A61K47/48 ,  A61P7/06 ,  C07K14/505 ,  C12N15/09 ,  C12P21/02 ,  C12N15/85

CPC分类号： A01K67/0275 ,  A01K2217/05 ,  A01K2217/052 ,  A01K2227/30 ,  A01K2267/01 ,  A01K2267/02 ,  A61K38/00 ,  A61K38/1816 ,  A61K47/60 ,  C07K14/005 ,  C07K14/505 ,  C12N7/00 ,  C12N15/8509 ,  C12N15/86 ,  C12N2015/8518 ,  C12N2740/10043 ,  C12N2740/10052 ,  C12N2760/20222 ,  C12N2799/027 ,  C12N2830/008

摘要： The present invention has for its object to provide a transgenic bird with a foreign gene containing a feline-derived protein-encoding sequence as transferred therein, and a method of producing the same. The present invention provides a method of producing a feline-derived protein by using a transgenic bird with a method which comprises infecting an avian embryo with a replication defective retrovirus vector containing a foreign gene by microinjection thereof into the early heart or blood vessel formed in the embryo and allowing the embryo to hatch.

公开(公告)号：EP2977387A1

公开(公告)日：2016-01-27

申请号：EP14769566.2

申请日：2014-03-17

申请人： Kazusa DNA Research Institute ,  Animal Allergy Clinical Laboratories Inc. ,  Nippon Zenyaku Kogyo Co., Ltd.

发明人： OHARA, Osamu ,  NAGASE, Takahiro ,  YAMAGUCHI, Yoshihiro ,  OHARA, Reiko ,  MASUDA, Kenichi ,  TSUKUI, Toshihiro

IPC分类号： C07K16/42 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N15/09 ,  C12P21/08 ,  G01N33/53

CPC分类号： C07K16/4291 ,  A01K2217/052 ,  A01K2227/706 ,  A01K2267/01 ,  C07K2317/14 ,  C07K2317/52 ,  C07K2317/54 ,  C07K2317/55 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/622 ,  C07K2317/624 ,  C07K2317/626 ,  C07K2319/21 ,  C12N15/8509 ,  C12N2015/8518 ,  G01N33/577 ,  G01N33/6854

摘要： Problem to be Solved: The present invention is intended to provide a polynucleotide encoding the light-chain variable region and the heavy-chain variable region of an anti-dog IgE antibody; and an anti-dog IgE antibody containing these variable regions.
Solution: The present invention is DNA encoding a heavy-chain variable region consisting of the amino acid sequence represented by SEQ ID NO: 2 or 6 and DNA encoding a light-chain variable region consisting of the amino acid sequence represented by SEQ ID NO: 4 or 8, and an anti-dog IgE monoclonal antibody which binds to dog IgE, containing these variable regions or a functional fragment thereof which binds to dog IgE.

摘要翻译： 要解决的问题：本发明的目的是提供编码轻链可变区和抗狗IgE的抗体的重链可变区的多核苷酸; 和抗犬的IgE抗体的合成含可变区。 解决方案：本发明是编码重链可变区由SEQ ID NO所代表的氨基酸序列的DNA：2或6和编码轻链可变区由SEQ ID NO所代表的氨基酸序列的DNA： 4或8，以及其结合狗IgE合成含可变区或其结合至IgE的狗的功能片段的抗狗的IgE单克隆抗体。

公开(公告)号：EP2761008A1

公开(公告)日：2014-08-06

申请号：EP12766714.5

申请日：2012-09-26

申请人： Kymab Limited

发明人： BRADLEY, Allan ,  LEE, E-Chiang ,  LIANG, Qi ,  SPENSBERGER, Dominik ,  ENGLAND, Nicholas

IPC分类号： C12N15/85 ,  C12N5/10 ,  C07K16/46

CPC分类号： C12N15/85 ,  A01K67/02 ,  A01K67/0278 ,  A01K2217/07 ,  A01K2217/077 ,  A01K2227/10 ,  A01K2227/105 ,  A01K2267/01 ,  C12N5/10 ,  C12N15/8509 ,  C12N2015/8518

摘要： The present invention relates inter alia to improvements in the production of chimaeric antibodies in non-human transgenic vertebrates such as mice and rats bearing one or more chimaeric antibody transgenes. In particular, the invention provides for improved non-human vertebrates and cells in which VpreB has been species-matched with the variable region of the chimaeric antibodies. Also, embodiments also provide for species-matching of the entire surrogate light chain for efficient pairing with chimaeric heavy chains during B-cell development in vivo in a non-human transgenic vertebrate setting.

公开(公告)号：EP2750496A2

公开(公告)日：2014-07-09

申请号：EP12826808.3

申请日：2012-08-30

申请人： Synageva BioPharma Corp.

发明人： LEIGHTON, Philip, A. ,  CADERA, Emily, J.

IPC分类号： A01K67/027

CPC分类号： A01K67/0278 ,  A01K67/0271 ,  A01K67/0275 ,  A01K67/0276 ,  A01K2207/15 ,  A01K2217/052 ,  A01K2217/072 ,  A01K2217/075 ,  A01K2217/15 ,  A01K2227/30 ,  A01K2267/01 ,  C07K16/00 ,  C07K16/02 ,  C07K16/462 ,  C07K2317/14 ,  C07K2317/21 ,  C07K2317/23 ,  C07K2317/24 ,  C07K2317/52 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/567 ,  C12N15/8509 ,  C12N2015/8518 ,  C12N2830/008

摘要： The invention relates to transgenic birds capable of producing chimeric immunoglobulins, with a combination of human and avian sequence, in their B cells. In some embodiments, the birds are chickens. When challenged with an antigen, the transgenic avians produce antigen-specific functional antibodies. The invention also relates to light chain immunoglobulin transgenes for making such transgenic avians, as well as methods and vectors for disrupting endogenous immunoglobulin loci in birds.