公开(公告)号：EP2402026A3

公开(公告)日：2012-04-18

申请号：EP11166299.5

申请日：2003-08-27

申请人： Intercell AG

发明人： Buschle, Michael ,  Habel, Andre ,  Klade, Christoph ,  Mattner, Frank ,  Otava, Oleksandr ,  Vytvytska, Oresta ,  Zauner, Wolfgang ,  Zinke, Sandra ,  Kirlappos, Helen

IPC分类号： A61K39/12 ,  A61K39/29 ,  A61K39/39 ,  A61K38/00 ,  A61P31/00 ,  A61K39/145 ,  C07K14/11 ,  C07K14/18 ,  A61K38/16 ,  A61K38/08

CPC分类号： C07K7/06 ,  A61K38/00 ,  A61K39/00 ,  A61K39/12 ,  A61K39/292 ,  A61K2039/525 ,  A61K2039/57 ,  C07K7/08 ,  C07K14/005 ,  C07K2319/00 ,  C12N2710/16122 ,  C12N2760/16122 ,  C12N2770/24222 ,  C12N2770/24234 ,  G01N2500/04 ,  Y10S930/223

摘要： Described is a method for isolating Hepatitis C Virus peptides (HPs) which have a binding capacity to a MHC/HLA molecule or a complex comprising said HCV-peptide and said MHC/HLA molecule characterized by the following steps: - providing a pool of HCV-peptide, said pool containing HCV-peptides which bind to said MHC/HLA molecules and HCV-peptides which do not bind to said MHC/HLA molecule, -contacting said MHC/HLA molecule with said pool of HCV-peptides whereby a HCV-peptide which has a binding capacity to said MHC/HLA molecule binds to said MHC/HLA molecule and a complex comprising said HCV-peptide and said MHC/HLA molecule is formed, -detecting and optionally separating said complex from the HCV-peptide which do not bind to said MHC/HLA molecule and optionally isolating and characterising the HCV-peptide from said complex.

公开(公告)号：EP2402026A2

公开(公告)日：2012-01-04

申请号：EP11166299.5

申请日：2003-08-27

申请人： Intercell AG

发明人： Buschle, Michael ,  Habel, Andre ,  Klade, Christoph ,  Mattner, Frank ,  Otava, Oleksandr ,  Vytvytska, Oresta ,  Zauner, Wolfgang ,  Zinke, Sandra ,  Kirlappos, Helen

IPC分类号： A61K39/12 ,  A61K39/29 ,  A61K39/39 ,  A61K38/00 ,  A61P31/00 ,  A61K39/145 ,  C07K14/11 ,  C07K14/18 ,  A61K38/16 ,  A61K38/08

CPC分类号： C07K7/06 ,  A61K38/00 ,  A61K39/00 ,  A61K39/12 ,  A61K39/292 ,  A61K2039/525 ,  A61K2039/57 ,  C07K7/08 ,  C07K14/005 ,  C07K2319/00 ,  C12N2710/16122 ,  C12N2760/16122 ,  C12N2770/24222 ,  C12N2770/24234 ,  G01N2500/04 ,  Y10S930/223

摘要： Described is a method for isolating Hepatitis C Virus peptides (HPs) which have a binding capacity to a MHC/HLA molecule or a complex comprising said HCV-peptide and said MHC/HLA molecule characterized by the following steps: - providing a pool of HCV-peptide, said pool containing HCV-peptides which bind to said MHC/HLA molecules and HCV-peptides which do not bind to said MHC/HLA molecule, -contacting said MHC/HLA molecule with said pool of HCV-peptides whereby a HCV-peptide which has a binding capacity to said MHC/HLA molecule binds to said MHC/HLA molecule and a complex comprising said HCV-peptide and said MHC/HLA molecule is formed, -detecting and optionally separating said complex from the HCV-peptide which do not bind to said MHC/HLA molecule and optionally isolating and characterising the HCV-peptide from said complex.

摘要翻译： 描述了一种用于分离对MHC / HLA分子具有结合能力的丙型肝炎病毒肽（HP）或包含所述HCV-肽和所述MHC / HLA分子的复合物的方法，其特征在于以下步骤：提供HCV池 所述库含有与所述MHC / HLA分子结合的HCV-肽和不与所述MHC / HLA分子结合的HCV-肽， - 使所述MHC / HLA分子与所述HCV-肽库接触，由此HCV- 对所述MHC / HLA分子具有结合能力的肽与所述MHC / HLA分子结合并形成包含所述HCV-肽和所述MHC / HLA分子的复合物， - 检测并任选地从HCV-肽中分离所述复合物， 不与所述MHC / HLA分子结合，并任选从所述复合物中分离和表征HCV-肽。