公开(公告)号：EP1535627A4

公开(公告)日：2005-12-14

申请号：EP03788098

申请日：2003-08-13

申请人： JAPAN SCIENCE & TECH AGENCY ,  JP NAT INST INFECTIOUS DISEASE ,  DEPT OF MEDICAL SCIENCES MINIS

发明人： HONDA MITSUO ,  MATSUO KAZUHIRO ,  KANEKIYO MASARU

IPC分类号： A61K39/04 ,  C07K14/16 ,  C07K16/10 ,  C12N15/31 ,  C12N15/67 ,  A61K39/00 ,  C12N15/11

CPC分类号： C07K14/005 ,  A61K39/04 ,  A61K2039/523 ,  C07K16/1054 ,  C12N15/67 ,  C12N2740/16222

摘要： A recombinant BCG vaccine obtained by transformation with an expression vector carrying a polynucleotide coding for an ecdemic antigenic protein, which recombinant BCG vaccine consists of a modified type polynucleotide comprising a polynucleotide having the third base of each of the codons thereof substituted with G or C without changing of the type of amino acid. This recombinant BCG vaccine excels in the amount of antigenic protein expressed, so that even with the same dosage as employed for conventional BCG vaccines, the recombinant BCG vaccine can induce satisfactory immune response to target infectious diseases, cancer, etc.

公开(公告)号：EP1457211A4

公开(公告)日：2006-06-14

申请号：EP01274763

申请日：2001-11-20

申请人： JAPAN SCIENCE & TECH AGENCY ,  JP NAT INST INFECTIOUS DISEASE

发明人： HONDA MITSUO ,  MATSUO KAZUHIRO ,  OHSU TAKEAKI ,  MIYAMURA TATSUO ,  MATSUURA YOSHIHARU ,  ISHII KOJI ,  KATO KENZO

IPC分类号： A61K39/00 ,  C07K14/16 ,  C12N7/01 ,  C12N7/08 ,  C12N15/49 ,  C12N15/863 ,  A61K39/285 ,  A61K39/21 ,  C12P21/00

CPC分类号： C12N7/00 ,  A61K39/00 ,  A61K2039/5256 ,  A61K2039/53 ,  C07K14/005 ,  C12N15/86 ,  C12N2710/24043 ,  C12N2740/16122

摘要： The invention provides a recombinant vaccinia virus strain DIs that possesses a polynucleotide encoding a foreign antigenic protein in the non-essential gene region of the chromosome DNA and expresses the antigenic protein; and provides a highly-safe, vaccinia virus vaccine containing the recombinant virus strain DIs as the active ingredient. The invention also provides a method of using the vaccinia virus strain DIs as a vector for protein expression.