公开(公告)号：EP1956087B1

公开(公告)日：2010-05-26

申请号：EP06810936.2

申请日：2006-09-29

申请人： JAPAN as represented by DIRECTOR GENERAL OF NATIONAL INSTITUTE OF INFECTIOUS DISEASES ,  Tokyo Metropolitan Organization for Medical Research ,  TORAY INDUSTRIES, INC.

发明人： TANABE, Jun-ichi ,  SONE, Saburo ,  WAKITA, Takaji, c/o Department of Virology II ,  ISHII, Koji ,  SUZUKI, Ryosuke ,  SUZUKI, Tetsuro ,  MIYAMURA, Tatsuo

IPC分类号： C12N15/09 ,  C12N7/00

CPC分类号： C12N7/00 ,  C12N15/86 ,  C12N2770/24243 ,  C12N2770/24252

公开(公告)号：EP1930416A1

公开(公告)日：2008-06-11

申请号：EP06810937.0

申请日：2006-09-29

申请人： JAPAN as represented by DIRECTOR GENERAL OF NATIONAL INSTITUTE OF INFECTIOUS DISEASES ,  Tokyo Metropolitan Organization for Medical Research ,  TORAY INDUSTRIES, INC.

发明人： TANABE, Jun-ichi, c/o Pharmaceutical Research Laboratories ,  SONE, Saburo ,  WAKITA, Takaji ,  ISHII, Koji ,  SUZUKI, Ryosuke ,  SUZUKI, Tetsuro ,  MIYAMURA, Tatsuo

IPC分类号： C12N7/00 ,  C12N15/09

CPC分类号： C12N7/00 ,  C12N15/86 ,  C12N2770/24223 ,  C12N2770/24243 ,  C12N2770/24252

摘要： The present invention relates to a method for producing a recombinant hepatitis C virus-like particle comprising the steps of introducing into (i) a cell in which an RNA replicon comprising a nucleotide sequence comprising the 5' untranslated region, the nucleotide sequence coding for the NS3, NS4A, NS4B, NS5A, and NS5B proteins, and the 3' untranslated region of a genome RNA derived from a hepatitis C virus strain autonomously replicates, (ii) a vector expressing the Core, E1, E2, and p7 proteins derived from a hepatitis C virus strain that is the same as or different from that as defined in the above (i), culturing the cell, and recovering the produced virus-like particle, and a recombinant hepatitis C virus particle produced by this method.

摘要翻译： 本发明涉及一种用于生产重组丙型肝炎病毒样颗粒，其包含引入（i）以RNA复制子的哪个包括包含5“非翻译区的核苷酸序列的细胞的步骤，所述核苷酸序列编码 NS3，NS4A，NS4B，NS5A和NS5B蛋白和3“从丙型肝炎病毒株自主复制来源的基因组RNA的非翻译区，（ii）表达核心，E1，E2，和P7蛋白的载体衍生自 丙型肝炎病毒株是做相同或不同，如上述（i）中所定义那样培养所述细胞，和回收所产生的病毒样颗粒，和由该方法生产的重组丙型肝炎病毒颗粒。

公开(公告)号：EP1956087A1

公开(公告)日：2008-08-13

申请号：EP06810936.2

申请日：2006-09-29

申请人： JAPAN as represented by DIRECTOR GENERAL OF NATIONAL INSTITUTE OF INFECTIOUS DISEASES ,  Tokyo Metropolitan Organization for Medical Research ,  TORAY INDUSTRIES, INC.

发明人： TANABE, Jun-ichic/oPharm. Res. Lab. ,  SONE, Saburo ,  WAKITA, Takaji, c/o Department of Virology II ,  ISHII, Koji ,  SUZUKI, Ryosuke ,  SUZUKI, Tetsuro ,  MIYAMURA, Tatsuo

IPC分类号： C12N15/09 ,  C12N7/00

CPC分类号： C12N7/00 ,  C12N15/86 ,  C12N2770/24243 ,  C12N2770/24252

摘要： The present invention relates to a method for producing infectious hepatitis C virus (HCV) particles, comprising a step of introducing an expression vector into a cell that allows HCV proliferation, such expression vector comprising: DNA sequences encoding the 5' untranslated region, structural proteins, and, if necessary, non-structural proteins of HCV and DNA sequences encoding non-structural proteins and the 3' untranslated region derived from the HCV JFH1 strain, which are located downstream of a polymerase I promoter; and a DNA fragment containing an RNA polymerase I terminator, which is located further downstream thereof.

摘要翻译： 本发明涉及一种生产感染性丙型肝炎病毒（HCV）颗粒的方法，包括将表达载体导入允许HCV增殖的细胞中的步骤，所述表达载体包含：编码5'非翻译区的DNA序列，结构蛋白 ，如果需要，HCV的非结构蛋白和编码非结构蛋白的DNA序列和源自HCV JFH1株的3'非翻译区位于聚合酶I启动子的下游; 和包含位于其下游的RNA聚合酶I终止子的DNA片段。

公开(公告)号：EP1930416B1

公开(公告)日：2011-12-14

申请号：EP06810937.0

申请日：2006-09-29

申请人： JAPAN AS REPRESENTED BY THE DIRECTOR-GENERAL OF NATIONAL INSTITUTE OF INFECTIOUS DISEASES ,  Tokyo Metropolitan Institute of Medical Science ,  TORAY INDUSTRIES, INC.

发明人： TANABE, Jun-ichi, c/o Pharmaceutical Research Laboratories ,  SONE, Saburo ,  WAKITA, Takaji, c/o Department of Virology II, National Institute ,  ISHII, Koji ,  SUZUKI, Ryosuke ,  SUZUKI, Tetsuro ,  MIYAMURA, Tatsuo

IPC分类号： C12N7/00 ,  C12N15/09 ,  C12N7/04

CPC分类号： C12N7/00 ,  C12N15/86 ,  C12N2770/24223 ,  C12N2770/24243 ,  C12N2770/24252

摘要： The invention relates to a method for producing a recombinant hepatitis C virus-like particle comprising introducing into (i) a cell which autonomously replicates an RNA replicon including a base sequence encoding a 5’ noncoding region, NS3 protein, NS4A protein, NS4B protein, NS5A protein and NS5B protein in the genomic RNA derived from a hepatitis C virus strain and a base sequence including a 3’ noncoding region, (ii) a vector expressing Core protein, E1 protein, E2 protein and p7 protein derived from the same or a different hepatitis C virus strain in (i), culturing the cell and collecting the produced virus-like particle and a recombinant hepatitis C virus particle produced by the method.