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公开(公告)号：EP3247794B1
公开(公告)日：2020-03-11
申请号：EP16739950.0
申请日：2016-01-21
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公开(公告)号：EP2877575B1
公开(公告)日：2018-06-27
申请号：EP13822148.6
申请日：2013-07-24
发明人： ASANO, Masahiro , YAGI, Toshihiro , FUKUI, Tsuyoshi , KAWASAKI, Atsuko , HATANO, Yukichi , MIHARA, Kazutoshi , SUGIMURA, Atsushi
IPC分类号： C12N9/40
CPC分类号： C12N9/2465
摘要： Disclosed is a method for production of recombinant human alpha-galactosidase A (rh alpha-Gal A) in a large scale, with a high purity. The method comprises the steps of (a) culturing rh alpha-Gal A-producing mammalian cells in a serum-free medium, (b) collecting culture supernatant, (c) subjecting the culture supernatant to anion-exchange column chromatography, (d) to hydrophobic column chromatography, (e) to a column chromatography employing as solid phase a material having affinity for phosphate group, (f) to cation-exchange column chromatography, and (g) to gel filtration column chromatography, in the order.
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公开(公告)号：EP2668272B1
公开(公告)日：2017-10-25
申请号：EP12739815.4
申请日：2012-01-23
发明人： MIHARA, Kazutoshi , KAWASAKI, Atsuko , OOTSUKI, Kouta , HATANO, Yuukichi , KAMEI, Shoichiro , SUGIMURA, Atsushi
CPC分类号： C12N9/16 , C12Y301/06013
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公开(公告)号：EP2877575A1
公开(公告)日：2015-06-03
申请号：EP13822148.6
申请日：2013-07-24
发明人： ASANO, Masahiro , YAGI, Toshihiro , FUKUI, Tsuyoshi , KAWASAKI, Atsuko , HATANO, Yukichi , MIHARA, Kazutoshi , SUGIMURA, Atsushi
IPC分类号： C12N9/40
CPC分类号： C12N9/2465
摘要： Disclosed is a method for production of recombinant human alpha-galactosidase A (rh alpha-Gal A) in a large scale, with a high purity. The method comprises the steps of (a) culturing rh alpha-Gal A-producing mammalian cells in a serum-free medium, (b) collecting culture supernatant, (c) subjecting the culture supernatant to anion-exchange column chromatography, (d) to hydrophobic column chromatography, (e) to a column chromatography employing as solid phase a material having affinity for phosphate group, (f) to cation-exchange column chromatography, and (g) to gel filtration column chromatography, in the order.
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公开(公告)号：EP3428284A1
公开(公告)日：2019-01-16
申请号：EP17763209.8
申请日：2017-03-07
发明人： KAKIMOTO, Shinji , MATEV, Miroslav , FUKUI, Tsuyoshi , HATANO, Yukichi , TANI, Junya , MIHARA, Kazutoshi , TAKAHASHI, Kenichi , SUGIMURA, Atsushi
摘要： Disclosed is a highly efficient method for production of highly pure mutant-type human erythropoietin. The method is for production of mutant-type human erythropoietin, in which a transformed mammalian cell is allowed to produce the mutant-type human erythropoietin, and the supernatant of the culture is subjected to hydrophobic column chromatography, multimodal anion exchange column chromatography, anion exchange column chromatography, phosphate group affinity column chromatography, and gel filtration column chromatography, in this order.
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6.发明公开METHOD FOR PURIFICATION OF RECOMBINANT HUMAN ALPHA-GALACTOSIDASE A FROM MATERIAL CONTAINING CONTAMINANT HOST CELL PROTEINS 审中-公开从含有污染性宿主细胞蛋白质的材料中纯化重组人αα-半乳糖苷酶A的方法
公开(公告)号：EP3247794A1
公开(公告)日：2017-11-29
申请号：EP16739950.0
申请日：2016-01-21
发明人： FUKUI, Tsuyoshi , KAWASAKI, Atsuko , HATANO, Yukichi , ITO, Yae , MIHARA, Kazutoshi , SUGIMURA, Atsushi
摘要： Disclosed is a method for production of recombinant human alpha-galactosidase A (rh alpha-Gal A) in a large scale, with a high purity. The method comprises the steps of (a) culturing rh alpha-Gal A-producing mammalian cells in a serum-free medium, (b) collecting culture supernatant, (c) subjecting the culture supernatant to anion-exchange column chromatography, (d) to hydrophobic column chromatography, (e) to a column chromatography employing as solid phase a material having affinity for phosphate group, (f) to cation-exchange column chromatography, (g) to dye-affinity column chromatography, and (h) to gel filtration column chromatography, in the order.
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