公开(公告)号：EP2351825A1

公开(公告)日：2011-08-03

申请号：EP09823714.2

申请日：2009-10-26

Applicant: Japan Science and Technology Agency

Inventor： IKUTA, Koji ,  INOUE, Yoshinori

IPC: C12M3/00 ,  B29C67/00 ,  B29C71/00 ,  C08J7/00

CPC classification number: B29C67/0066 ,  B29C64/135 ,  B29C64/20 ,  B29C71/02 ,  B29C71/04 ,  B29C2035/0827 ,  B29K2995/0094 ,  B29L2031/7532 ,  B33Y70/00 ,  C12M23/20 ,  Y10T428/24

Abstract: The present invention provides a means to detoxify and add cytocompatibility to three-dimensional microstereolithographic objects fabricated by microstereolithography. Cytocompatibility is a property of a material in that the material is non-toxic to cells and does not have an adverse effect on the activities of living cells.
In the present invention, three-dimensional microstereolithographic objects fabricated by microstereolithography are exposed to UV light for one hour to accelerate hardening. The structures are then heated at 175 °C or above for at least 6 hours. The heating temperature may exceed the glass transition temperature, which is the index of thermal softening temperature of materials. The present invention relates to three-dimensional microstructures formed by microstereolithography. Deformation due to a structure's own weight, which generally poses a problem in heat treatment, is reduced with microstructures due to the size effect. As a result, the dimensions of the three-dimensional structure fabricated by the present invention remain almost unchanged before and after the heat treatment.

Abstract translation: 本发明提供了一种手段来解毒和细胞相容性添加到由microstereolithography microstereolithographic制造三维物体。 细胞相容性是材料的特性，裸片DASS材料是无毒的细胞和不具有对活细胞的活性产生不利影响。 另外，在本发明中，通过microstereolithography microstereolithographic制造三维物体暴露于UV光一小时，以加速硬化。 该结构然后在175℃或以上加热至少6小时。 加热温度可以超过玻璃化转变温度，所有这些是材料的热软化温度的索引。 本发明涉及通过microstereolithography形成的三维微结构。 由于结构本身的重量，这基因的反弹造成的热处理变形问题，用微观由于尺寸效应降低。 其结果是，所述三维结构的由本发明制造的尺寸之前和热处理之后几乎保持不变。

公开(公告)号：EP2351825B1

公开(公告)日：2017-05-10

申请号：EP09823714.2

申请日：2009-10-26

Applicant: Japan Science and Technology Agency

Inventor： IKUTA, Koji ,  INOUE, Yoshinori

IPC: B29C67/00 ,  B29C71/00 ,  B29C71/02 ,  B29C71/04 ,  B29C35/08 ,  B29L31/00 ,  C08J7/00 ,  C12M3/00

CPC classification number: B29C67/0066 ,  B29C64/135 ,  B29C64/20 ,  B29C71/02 ,  B29C71/04 ,  B29C2035/0827 ,  B29K2995/0094 ,  B29L2031/7532 ,  B33Y70/00 ,  C12M23/20 ,  Y10T428/24

公开(公告)号：EP2618197B1

公开(公告)日：2016-06-08

申请号：EP11824911.9

申请日：2011-07-27

Applicant: Japan Science and Technology Agency

Inventor： IKUTA, Koji ,  IKEUCHI, Masashi

IPC: G02B21/00 ,  G02B21/32 ,  G02B21/36

CPC classification number: G02B21/367 ,  B33Y80/00 ,  G02B21/006 ,  G02B21/0076 ,  G02B21/32 ,  G02B21/365

Abstract: Provided is a 3-dimensional confocal microscopy apparatus which is manufactured by combining a confocal microscope and an optical tweezers technique, wherein a pair of lenses for focal plane displacement where one lens is movable in the optical axis direction is arranged between a fixed objective lens and a fluorescent light imaging camera, and the 3-dimensional confocal microscopy apparatus also includes a mean which corrects the aberration of a fluorescent confocal image obtained by the fluorescent imaging camera. Accordingly, it is possible to provide a 3-dimensional confocal microscopy apparatus which can acquire a 3-dimensional image of a specimen during a manipulation of the specimen using optical tweezers without affecting an optical trap.

Abstract translation: 本发明提供一种三维共聚焦显微镜装置的所有其通过结合共焦显微镜和光学镊子技术制造，worin一对透镜焦平面位移，其中一个透镜是在光轴方向上移动是一个固定物镜和之间布置 因此荧光灯成像相机和所述三维共聚焦显微镜装置包括：平均校正因荧光成像相机获得的荧光的共焦图像的像差。 因此，有可能提供一种三维共聚焦显微镜装置使用光学镊子试样的操作，在此期间能够获得的试样的3维图像，而不会影响到光阱。