公开(公告)号：EP1865325B1

公开(公告)日：2012-05-16

申请号：EP05816850.1

申请日：2005-12-15

申请人： Japan Science and Technology Agency ,  Local Government of Aichi Prefecture ,  WAKO PURE CHEMICAL INDUSTRIES LIMITED

发明人： KANNAGI, Reiji ,  IZAWA, Mineko ,  KIMURA, Naoko ,  KUREI, Shunsuke ,  WATANABE, Kazue

IPC分类号： G01N33/574 ,  C07K16/30

CPC分类号： C07K16/40 ,  C07K16/3076 ,  G01N33/57407 ,  G01N2333/91194 ,  G01N2400/02

摘要： [PROBLEMS] To provide a method of screening, and a test agent, capable of detecting cancer patients and patients with a high risk of cancer at high percentage. [MEANS FOR SOLVING PROBLEMS] A remarkable difference in the distribution of sugar chain sulfating enzyme GlcNAc-6-sulfotransferase isozymes has been found between noncancerous tissues and cancer/adenoma tissues. By the use of the same, it has been clarified that cancer and adenoma (excluding colon cancer and colon adenoma) can be specifically detected through detection of a given range of GlcNAc-6-sulfated sugar chains from patient tissues or fecal specimens. Cancer and adenoma screening can be carried out by the use of an antibody capable of reacting with GlcNAc-6-sufated sugar chains specifically formed by enzymes occurring in cancer and adenoma tissues.

公开(公告)号：EP1865325A1

公开(公告)日：2007-12-12

申请号：EP05816850.1

申请日：2005-12-15

申请人： Japan Science and Technology Agency ,  Local Gavernment of Aichi Prefecture

发明人： KANNAGI, Reiji ,  IZAWA, Mineko ,  KIMURA, Naoko ,  KUREI, Shunsuke ,  WATANABE, Kazue

IPC分类号： G01N33/574 ,  C07K16/30

CPC分类号： C07K16/40 ,  C07K16/3076 ,  G01N33/57407 ,  G01N2333/91194 ,  G01N2400/02

摘要： [PROBLEMS] To provide examination methods and reagents able to detect efficiently cancer patients and patients at high risk of cancer.
[MEANS FOR SOLVING PROBLEMS] Significant differences in the distribution of GlcNAc-6-sulfotransferase isozymes, sulfation enzymes of sugar residues, between non-carcinoma tissues and carcinoma tissues or adenoma tissues were discovered. The discovery is evidently applicable to detect carcinomas and adenomas (except colorectal carcinomas and colorectal adenomas) specifically by assaying a certain range of GlcNAc-6-sulfated sugar residue groups in tissues of patients and in fecal samples. Examination of carcinomas and adenomas is possible by the use of antibodies reacting specifically with GlcNAc-6-sulfated sugar residues specifically synthesized by enzymes present in carcinoma and adenoma tissues.

摘要翻译： [问题]提供能够有效检测癌症患者和癌症高危患者的检查方法和试剂。 [解决问题的方法]发现了GlcNAc-6-磺基转移酶同工酶，糖残基硫酸化酶，非癌组织和癌组织或腺瘤组织分布的显着差异。 该发现显然适用于通过测定患者组织和粪便样品中特定范围的GlcNAc-6硫酸化糖残基来检测癌和腺瘤（结肠直肠癌和结肠直肠腺瘤除外）。 可以通过使用特异性地与存在于癌和腺瘤组织中的酶特异性合成的GlcNAc-6硫酸化糖残基的抗体来检查癌和腺瘤。