公开(公告)号：EP3409789A1

公开(公告)日：2018-12-05

申请号：EP17743725.8

申请日：2017-01-24

申请人： Jianghan University ,  Children's Hospital Medical Center

发明人： PENG, Hai ,  ZHANG, Ying ,  LU, Long

IPC分类号： C12Q1/68 ,  C12Q1/06 ,  C12Q1/04 ,  C12Q1/70

CPC分类号： C12Q1/04 ,  C12Q1/06 ,  C12Q1/68 ,  C12Q1/70

摘要： The present invention discloses a method for qualitative and quantitative detection of a microorganism in a human body, which belongs to the field of biotechnology. The method includes the following steps: determining a target microbial population, a target microorganism and a non-target organism in a sample to be tested, as well as a reference microorganism not present in the sample to be tested; designing the characteristic regions of the target microbial population and the target microorganism; designing multiplex amplification primers for the characteristic regions; adding the reference microorganism and an exogenous nucleic acid into the sample to be tested, and then extracting the nucleic acid of the microorganism in the sample to be tested; amplifying the nucleic acid of the microorganism with the designed multiplex amplification primers so as to obtain a characteristic sequencing fragment; and then performing, using the characteristic sequencing fragment, qualitative and quantitative analysis for the microorganism in the sample to be tested. The present invention does not need pre-culture and proliferation of the microorganism, and can perform high throughput, high accuracy and high resolution detection on a plurality of known microorganisms in the sample to be tested at one time, and the detection process is simple, quick and the process is standardized.