公开(公告)号：EP1418182A1

公开(公告)日：2004-05-12

申请号：EP02760561.7

申请日：2002-08-07

申请人： Juridical Foundation, The Chemo-Sero-Therapeutic Research Institute

发明人： KAMEI, Shintaro ,  HAMAMOTO, Takayoshi ,  HIRASHIMA, Masaki ,  MAEDA, Hiroaki ,  TAKAHASHI, Kazuhiko

IPC分类号： C07K14/47 ,  C07K1/16

CPC分类号： C07K1/22 ,  A61K38/00 ,  C07K1/16 ,  C07K1/26 ,  C07K1/36 ,  C07K14/47

摘要： A process for preparing a peptide fragment having a cell death-inhibitory activity is provided. In order to obtain a selenoprotein P fragment having a cell death-inhibitory activity, full-length selenoprotein P was reacted with various serine proteinases and the resulting fragments were electrophoresed and assessed for a cell death-inhibitory activity. A process for preparing a selenoprotein P fragment was established by identifying an enzyme that produced bands corresponding to a molecular weight of not more than 3.5 x 10 4 in electrophoresis. A process for preparing a peptide fragment having a cell death-inhibitory activity according to the present invention may be used for amelioration, treatment and prevention of diseases caused by cell death and for more efficient production of useful living substances.

摘要翻译： 提供了制备具有细胞死亡抑制活性的肽片段的方法。 为了获得具有细胞死亡抑制活性的硒蛋白P片段，使全长硒蛋白P与各种丝氨酸蛋白酶反应，并对所得片段进行电泳并评估细胞死亡抑制活性。 通过鉴定在电泳中产生对应于分子量不超过3.5×10 4的条带的酶，建立了硒蛋白P片段的制备方法。 根据本发明的用于制备具有细胞死亡抑制活性的肽片段的方法可用于改善，治疗和预防由细胞死亡引起的疾病并用于更有效地生产有用的生物。