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1.发明公开METHOD OF PREPARING PEPTIDE FRAGMENT HAVING CELL DEATH INHIBITORY ACTIVITY 审中-公开制备具有细胞死亡抑制活性的肽片段的方法
公开(公告)号：EP1418182A1
公开(公告)日：2004-05-12
申请号：EP02760561.7
申请日：2002-08-07
发明人： KAMEI, Shintaro , HAMAMOTO, Takayoshi , HIRASHIMA, Masaki , MAEDA, Hiroaki , TAKAHASHI, Kazuhiko
摘要： A process for preparing a peptide fragment having a cell death-inhibitory activity is provided. In order to obtain a selenoprotein P fragment having a cell death-inhibitory activity, full-length selenoprotein P was reacted with various serine proteinases and the resulting fragments were electrophoresed and assessed for a cell death-inhibitory activity. A process for preparing a selenoprotein P fragment was established by identifying an enzyme that produced bands corresponding to a molecular weight of not more than 3.5 x 10 4 in electrophoresis. A process for preparing a peptide fragment having a cell death-inhibitory activity according to the present invention may be used for amelioration, treatment and prevention of diseases caused by cell death and for more efficient production of useful living substances.
摘要翻译： 提供了制备具有细胞死亡抑制活性的肽片段的方法。 为了获得具有细胞死亡抑制活性的硒蛋白P片段，使全长硒蛋白P与各种丝氨酸蛋白酶反应，并对所得片段进行电泳并评估细胞死亡抑制活性。 通过鉴定在电泳中产生对应于分子量不超过3.5×10 4的条带的酶，建立了硒蛋白P片段的制备方法。 根据本发明的用于制备具有细胞死亡抑制活性的肽片段的方法可用于改善，治疗和预防由细胞死亡引起的疾病并用于更有效地生产有用的生物。
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公开(公告)号：EP1437410A1
公开(公告)日：2004-07-14
申请号：EP02767954.7
申请日：2002-09-12
发明人： KAMINAKA, Sara , KAMINAKA, Kazuyoshi , HIRASHIMA, Masaki , MAEDA, Hiroaki , NOZAKI, Chikateru , TAKAHASHI, Kazuhiko
CPC分类号： C12N9/0065 , C07K14/765 , C07K2319/00
摘要： A novel selenocysteine-containing protein is provided which is a substantial entity having an enzymatic activity, typically an activity to reduce peroxide phospholipids. There are provided a gene consisting of a coding sequence for a protein not containing selenocysteine in which the codon for selenocysteine is introduced at a desired position and a selenocysteine insertion sequence resided at the 3'-end of said coding sequence and a protein expressed therefrom, typically a selenocysteine-containing protein having an activity to reduce peroxide phospholipids which is prepared by introducing selenocysteine into albumin and as well as a gene encoding said protein. A novel anti-oxidative substance is provided which can be applied for prevention of worsening, prophylaxis or treatment of various diseases associated with oxidative stress.
摘要翻译： 含有通过将一个或多个硒代半胱氨酸插入或取代含有硒代半胱氨酸的蛋白质并具有酶活性的骨架形成的重组硒蛋白的蛋白质是新的。 还包括以下独立权利要求：（1）编码这种重组的含半胱氨酸的蛋白质的基因; 和（2）通过使用该基因产生重组的含半胱氨酸的蛋白质。 活性：免疫调节剂; 抑制细胞生长; Cardiant; 精神药物; 神经保护; 降血糖; 眼科。 行动机制：源材料无。
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