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1.发明公开
公开(公告)号：EP0773030A1
公开(公告)日：1997-05-14
申请号：EP95921113.7
申请日：1995-06-07
CPC分类号： C07K14/285 , A61K39/00 , C07K14/235
摘要： The present invention aims at providing a method for preparing dermonecrotic toxin, an improved toxoid of dermonecrotic toxin produced by Bordetella and a toxoid mixture comprising said improved toxoid of dermonecrotic toxin produced by Bordetella and a toxoid of dermonecrotic toxin produced by Pasteurella .
There is provided a method for partially purifying dermonecrotic toxin which comprises bringing a dermonecrotic toxin-containing solution into contact with a chromatographic gel sulfated by direct sulfation or a chromatographic gel to which a sulfated molecule is covalently bonded to thereby make the dermonecrotic toxin adsorbed on the gel, and eluting the adsorbed dermonecrotic toxin from the gel. There is also provided a toxoid obtained from dermonecrotic toxin produced by Bordetella , said dermonecrotic toxin being prepared by the above method for partially purifying dermonecrotic toxin, and a toxoid mixture prepared by mixing said toxoid and a toxoid of dermonecrotic toxin produced by toxigenic Pasteurella , said toxoid mixture being safe and effective for preventing the atrophic rhinitis in pigs.摘要翻译： 本发明的目的在于提供一种制备皮肤坏死毒素的方法，由博代氏杆菌产生的皮肤坏死毒素的改良类毒素和包含所述改良的博德特氏菌产生的皮肤坏死毒素类毒素和由巴氏杆菌产生的皮肤坏死毒素类毒素的类毒素混合物。 提供了一种部分纯化皮肤坏死毒素的方法，该方法包括使含有皮肤坏死毒素的溶液与通过直接硫酸化硫酸化的色谱凝胶或与硫酸化分子共价结合的色谱凝胶接触，从而使吸附在皮肤坏死毒素上的皮肤坏死毒素 凝胶，并从凝胶上洗脱吸附的皮肤坏死毒素。 还提供了由博德特氏菌产生的皮肤坏死毒素获得的类毒素，所述皮肤坏死毒素通过上述部分纯化皮肤坏死毒素的方法制备，以及通过混合所述类毒素和由产毒巴斯德氏菌产生的皮肤坏死毒素的类毒素制备的类毒素混合物，所述 类毒素混合物对预防猪萎缩性鼻炎安全有效。
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2.发明授权PROCESS FOR PURIFYING DERMONECROTIC TOXIN PRODUCED BY BORDETELLA BRONCHISEPTICA 失效支气管败血波氏杆菌清洗方法产生皮肤坏死毒素
公开(公告)号：EP0773030B8
公开(公告)日：2004-01-14
申请号：EP95921113.7
申请日：1995-06-07
CPC分类号： C07K14/285 , A61K39/00 , C07K14/235
摘要： The invention aims at providing a process for preparing a necrotoxin, an improved toxoid of a necrotoxin produced by a bordetella, and a toxoid mixture comprising the improved toxoid and a toxoid of a necrotoxin produced by a pasteurella. The invention provides a method of partially purifying a necrotoxin by bringing a necrotoxin-containing solution into contact with either a chromatographic carrier gel sulfated by direct sulfation or a chromatographic carrier gel covalently bonded to a sulfated molecule to thereby make the necrotoxin adsorbed on the gel, followed by the elution thereof from the gel. Also provided are a toxoid derived from the necrotoxin produced by a bordetella and partially purified by the above purification method and a toxoid mixture which comprises the above toxoid and a toxoid derived from a necrotoxin produced by a toxigenic pasteurella and is safe for swine and efficacious in preventing atrophic rhinitis.
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公开(公告)号：EP0773030B1
公开(公告)日：2003-09-03
申请号：EP95921113.7
申请日：1995-06-07
CPC分类号： C07K14/285 , A61K39/00 , C07K14/235
摘要： The invention aims at providing a process for preparing a necrotoxin, an improved toxoid of a necrotoxin produced by a bordetella, and a toxoid mixture comprising the improved toxoid and a toxoid of a necrotoxin produced by a pasteurella. The invention provides a method of partially purifying a necrotoxin by bringing a necrotoxin-containing solution into contact with either a chromatographic carrier gel sulfated by direct sulfation or a chromatographic carrier gel covalently bonded to a sulfated molecule to thereby make the necrotoxin adsorbed on the gel, followed by the elution thereof from the gel. Also provided are a toxoid derived from the necrotoxin produced by a bordetella and partially purified by the above purification method and a toxoid mixture which comprises the above toxoid and a toxoid derived from a necrotoxin produced by a toxigenic pasteurella and is safe for swine and efficacious in preventing atrophic rhinitis.
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