公开(公告)号：EP2189790A1

公开(公告)日：2010-05-26

申请号：EP08827849.4

申请日：2008-08-20

申请人： Juridical Foundation The Chemo-Sero-Therapeutic Research Institute ,  JAPAN as represented by DIRECTOR GENERAL OF NATIONAL INSTITUTE OF INFECTIOUS DISEASES

发明人： TORII, Yasushi ,  HARAKAWA, Tetsuhiro ,  GOTO, Yoshitaka ,  GINNAGA, Akihiro ,  KAJI, Ryuji ,  KOZAKI, Shunji ,  TAKAHASHI, Motohide

IPC分类号： G01N33/48 ,  G01N33/15

CPC分类号： G01N33/6854 ,  A61B5/0488 ,  G01N2333/33 ,  G01N2469/20

摘要： A method for quantification of a titer of a neutralizing antibody to a neurotoxin is disclosed. The method comprises the steps: (a) mixing a standard sample containing a fixed amount of a neurotoxin and a test sample containing a neutralizing antibody to said neurotoxin; (b) administering the mixture obtained in step (a) into the muscle of a non-human mammal; (c) applying electric stimulus to said non-human mammal; (d) measuring a compound muscle action potential (CMAP) due to contraction of the muscle of said mammal by application of electric stimulus with an electromyograph; and (e) analyzing CMAP amplitude data obtained in step (d) for an extent of decrease in amplitude by a non-neutralized neurotoxin to thereby quantify a titer of the neutralizing antibody contained in the test sample.

摘要翻译： 公开了定量测定神经毒素中和抗体效价的方法。 该方法包括以下步骤：（a）将含有固定量的神经毒素的标准样品和含有中和抗体的测试样品混合到所述神经毒素中; （b）将步骤（a）中获得的混合物给予非人类哺乳动物的肌肉; （c）对所述非人类哺乳动物施加电刺激; （d）通过用肌电图应用电刺激测量所述哺乳动物肌肉收缩引起的复合肌肉动作电位（CMAP）; 和（e）分析步骤（d）中获得的CMAP幅度数据对于未中和的神经毒素的幅度降低程度，从而定量测试样品中含有的中和抗体的效价。

公开(公告)号：EP2189790B1

公开(公告)日：2016-07-20

申请号：EP08827849.4

申请日：2008-08-20

申请人： The Chemo-Sero-Therapeutic Research Institute ,  JAPAN as represented by DIRECTOR GENERAL OF NATIONAL INSTITUTE OF INFECTIOUS DISEASES

发明人： TORII, Yasushi ,  HARAKAWA, Tetsuhiro ,  GOTO, Yoshitaka ,  GINNAGA, Akihiro ,  KAJI, Ryuji ,  KOZAKI, Shunji ,  TAKAHASHI, Motohide

IPC分类号： G01N33/48 ,  G01N33/15 ,  G01N33/50 ,  G01N33/68

CPC分类号： G01N33/6854 ,  A61B5/0488 ,  G01N2333/33 ,  G01N2469/20