-
公开(公告)号：EP3492493A1
公开(公告)日：2019-06-05
申请号：EP17834020.4
申请日：2017-07-11
申请人： Kaneka Corporation
发明人： MURATA, Dai , YOSHIDA, Shinichi
摘要： The objective of the present invention is to provide a method for efficiently producing a target antibody fragment having high purity by separating an impurity which has a similar physical property to the target antibody fragment and of which separation is difficult. The method for producing an antibody fragment according to the present invention is characterized in that the antibody fragment comprises a CH1 region and does not comprise an Fc region, and characterized in comprising the steps of preparing a liquid sample, wherein the liquid sample comprises the antibody fragment and does not comprise an Fc fragment, contacting the liquid sample with an affinity separation matrix in order to adsorb the antibody fragment on the affinity separation matrix, wherein Protein G, a Protein G domain, a Protein G variant or a Protein G domain variant is immobilized as a ligand on a water-insoluble carrier in the affinity separation matrix, washing the affinity separation matrix to remove an impurity, and separating the antibody fragment from the affinity separation matrix.
-
2.发明公开
公开(公告)号：EP2690106A1
公开(公告)日：2014-01-29
申请号：EP12760318.1
申请日：2012-03-23
申请人： Kaneka Corporation
发明人： MURATA, Dai , YOSHIDA, Shinichi
IPC分类号： C07K1/22 , B01J20/22 , B01J20/281 , G01N30/88
CPC分类号： C07K17/00 , B01D15/3809 , B01J20/289 , B01J20/3202 , B01J20/3219 , B01J20/3246 , C07K1/22
摘要： The low-molecular-weight compound represented by the general formula (1): ArX-(Linker)-ArYHB (1), wherein ArX is a structure containing an optionally substituted aromatic six-membered ring, ArYHB is a structure containing an optionally substituted aromatic six-membered ring having a proton donor, the atom group "Linker" has not less than 4 and not more than 30 atoms and binds ArX with ArYHB, is used for binding a proteinaceous substance.
-
公开(公告)号：EP3040344A1
公开(公告)日：2016-07-06
申请号：EP14841163.0
申请日：2014-08-28
申请人： Kaneka Corporation
发明人： YOSHIDA, Shinichi , MURATA, Dai
CPC分类号： C07K16/1275 , C07K1/22 , C07K14/315 , C07K2317/55 , C12N2510/02
摘要： An object of the present invention is to provide a Fab region-binding peptide having an excellent ability for binding to a Fab region of IgG, an affinity separation matrix having the peptide as a ligand, and a method for producing a Fab region-containing protein, the method using the affinity separation matrix. Further, another object of the present invention is to provide a DNA encoding for the peptide, a vector containing the DNA, and a transformant which has been transformed by the vector. The Fab region-binding peptide according to the present invention is characterized in having a mutation at a specific site in comparison with wild-type SpG-β1.
摘要翻译： 本发明的目的是提供具有优异的结合IgG Fab区的Fab区结合肽，具有该肽作为配体的亲和分离基质，以及含有Fab区的蛋白质的制造方法 ，使用亲和力分离矩阵的方法。 此外，本发明的另一个目的是提供编码肽的DNA，含有DNA的载体和已经通过载体转化的转化体。 根据本发明的Fab区结合肽的特征在于与野生型SpG-21相比在特定位点具有突变。
-
公开(公告)号：EP4206329A1
公开(公告)日：2023-07-05
申请号：EP21861155.6
申请日：2021-08-03
申请人： KANEKA CORPORATION
摘要： The objective of the present invention is to provide a method for purifying a useful substance with effectively suppressing the occurrence of precipitate after an impurity is adsorbed to be removed on the specific adsorbent from a useful substance-containing liquid. The method for purifying a useful substance from a useful substance-containing liquid according to the first present invention is characterized in comprising the steps of contacting the useful substance-containing liquid with a hardly-soluble magnesium compound, removing the hardly-soluble magnesium compound from the useful substance-containing liquid contacted with the hardly-soluble magnesium compound to obtain a treated liquid, and adjusting pH of the treated liquid to 8.0 or less. The method for purifying a useful substance from a useful substance-containing liquid according to the second present invention is characterized in comprising the steps of contacting the useful substance-containing liquid with a hardly-soluble magnesium compound, and contacting the useful substance-containing liquid with an aluminum compound.
-
公开(公告)号：EP2690108B1
公开(公告)日：2018-07-25
申请号：EP12765583.5
申请日：2012-03-26
申请人： Kaneka Corporation
发明人： YOSHIDA, Shinichi , MURATA, Dai , TAIRA, Shunichi
摘要： An object of the present invention is to provide novel polypeptides that are capable of binding to an immunoglobulin and have high stability against alkali. The present invention relates to proteins having the amino acid sequence of SEQ ID No:1 or 2.
-
公开(公告)号：EP2690108A1
公开(公告)日：2014-01-29
申请号：EP12765583.5
申请日：2012-03-26
申请人： Kaneka Corporation
发明人： YOSHIDA, Shinichi , MURATA, Dai , TAIRA, Shunichi
摘要： An object of the present invention is to provide novel polypeptides that are capable of binding to an immunoglobulin and have high stability against alkali. The present invention relates to proteins having the amino acid sequence of SEQ ID No:1 or 2.
-
-
-
-
-
搜索结果
6 条记录 (耗时 0.012 秒)
