公开(公告)号：EP1914315B1

公开(公告)日：2013-02-27

申请号：EP06781266.9

申请日：2006-07-19

申请人： KIKKOMAN CORPORATION

发明人： HIROKAWA, Kozo, Kikkoman Corporation ,  SHIMOJI, Kazuhiko, Kikkoman Corporation

IPC分类号： G01N33/50 ,  C12Q1/26 ,  C12Q1/37

CPC分类号： C12Q1/26 ,  C12Q1/37 ,  G01N2400/00

公开(公告)号：EP1914315A1

公开(公告)日：2008-04-23

申请号：EP06781266.9

申请日：2006-07-19

申请人： KIKKOMAN CORPORATION

发明人： HIROKAWA, Kozo, Kikkoman Corporation ,  SHIMOJI, Kazuhiko, Kikkoman Corporation

IPC分类号： C12Q1/26 ,  C12Q1/37

CPC分类号： C12Q1/26 ,  C12Q1/37 ,  G01N2400/00

摘要： The present invention relates to a method for quantitative determination of an α-glycated peptide in a sample, comprising causing protease to act on a whole blood and/or blood cell sample, causing an elimination reagent containing one or a plurality of types of ketoamine oxidase to act on the resultant, eliminating an α-glycated amino acid, an ε-glycated amino acid, an ε-glycated peptide, or a combination thereof, and then determining the α-glycated peptide in the sample using oxidase that acts on the α-glycated peptide. The present invention also relates to an elimination reagent and a kit to be used for such method. According to the present invention, measurement errors in quantitative determination of a glycated protein such as glycated hemoglobin can be reduced, and thus a precise measured value can be obtained.

摘要翻译： 本发明涉及一种定量测定样品中的α-糖化肽的方法，该方法包括使蛋白酶作用于全血和/或血细胞样品，产生含有一种或多种类型的酮胺氧化酶 作用于所得物上，除去α-糖化氨基酸，ε-糖化氨基酸，ε-糖化肽或其组合，然后使用作用于α的氧化酶测定样品中的α-糖化肽 - 糖化肽。 本发明还涉及用于这种方法的消除试剂和试剂盒。 根据本发明，能够降低糖化血红蛋白等糖化蛋白质的定量测定误差，能够得到精确的测定值。