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1.发明公开VERFAHREN ZUR HERSTELLUNG VON KLONOGENEN FIBROBLASTEN, VERFAHREN ZUR GENTRANSFIZIERUNG VON FIBROBLASTEN UND SO ERHALTENE GENTRANSFIZIERTE FIBROBLASTEN 失效工艺用于生产成纤维细胞克隆形成GENTRANSFIZIERUNG成纤维细胞合成得到的基因转染的成纤维细胞
公开(公告)号：EP0748374A1
公开(公告)日：1996-12-18
申请号：EP95909773.0
申请日：1995-02-23
发明人： ROSENTHAL, Felicia, M. , LINDEMANN, Albrecht , BOEHM, Thomas , MERTELSMANN, Roland , VEELKEN, Hendrik , KULMBURG, Peter
IPC分类号： C12N15 , A61K9 , A61K31 , A61K35 , A61K38 , A61K47 , A61K48 , A61P43 , C07H21 , C07K14 , C12N5
CPC分类号： C12N5/0656 , A61K38/00 , A61K48/00 , C07K14/535 , C12N2510/02
摘要： In a method for preparing clonogenic fibroblasts, tissue is taken from the donor and the cells isolated from the tissue, the cell suspension so obtained is sieved, the cells contained in the cell suspension are grown and the cells are transferred to a tissue culture; alternatively, the cells are isolated by mechanical comminution followed by enzymatic treatment with collagenase alone. As a result of the transfection into the fibroblasts, at least one gene which codes for a biologically active protein, preferably a therapeutically active protein such as a growth factor, a hormone, an enzyme, a clotting factor or a clotting inhibitor, is inwardly transferred.
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