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公开(公告)号：EP0390592A2
公开(公告)日：1990-10-03
申请号：EP90303445.2
申请日：1990-03-30
发明人： Sekine, Susumu , Ito, Seiga , Katsuki, Motoya
CPC分类号： C12N15/8509 , A01K67/0278 , A01K2207/15 , A01K2217/00 , A01K2227/10 , A01K2227/105 , A01K2267/01 , C07K14/4732 , C07K14/595 , C07K2319/00 , C07K2319/02 , C12N9/6462 , C12N15/70 , C12N15/85 , C12Y304/21073
摘要： A recombinant vector suitable for industrially making an animal produce useful protein in the milk, which is constructed by extracting high molecular weight DNA from the bovine liver to prepare a chromosomal gene library, preparing a synthetic DNA probe corresponding to the publicly known bovine αS1 casein chromosomal gene, screening a clone carrying bovine αS1 casein chromosomal gene, confirming that the clone has a base sequence coincident with that of the publicly known bovine αS1 casein chromosomal gene, ligating human prourokinase cDNA with the promoter part and the intron 1 part of the gene, and then inserting the obtained plasmid into a suitable vector. A transgenic mouse prepared by using this recombinant vector secreted a considerable amount of human prourokinase as useful protein in its milk after delivery.
摘要翻译： 适用于工业制造动物的重组载体通过从牛肝中提取高分子量DNA以制备染色体基因文库，制备对应于公知牛αS1酪蛋白的合成DNA探针而在乳中产生有用的蛋白质 染色体基因，筛选携带牛αS1酪蛋白染色体基因的克隆，证实该克隆具有与公知的牛α1S酪蛋白染色体基因一致的碱基序列，将人类prourokinase cDNA与启动子部分和内含子1部分连接 该基因，然后将获得的质粒插入合适的载体中。 通过使用该重组载体制备的转基因小鼠在分娩后在其牛奶中分泌大量的人类尿激酶作为有用的蛋白质。
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公开(公告)号：EP0390592A3
公开(公告)日：1991-06-12
申请号：EP90303445.2
申请日：1990-03-30
发明人： Sekine, Susumu , Ito, Seiga , Katsuki, Motoya
CPC分类号： C12N15/8509 , A01K67/0278 , A01K2207/15 , A01K2217/00 , A01K2227/10 , A01K2227/105 , A01K2267/01 , C07K14/4732 , C07K14/595 , C07K2319/00 , C07K2319/02 , C12N9/6462 , C12N15/70 , C12N15/85 , C12Y304/21073
摘要： A recombinant vector suitable for industrially making an animal produce useful protein in the milk, which is constructed by extracting high molecular weight DNA from the bovine liver to prepare a chromosomal gene library, preparing a synthetic DNA probe corresponding to the publicly known bovine αS1 casein chromosomal gene, screening a clone carrying bovine αS1 casein chromosomal gene, confirming that the clone has a base sequence coincident with that of the publicly known bovine αS1 casein chromosomal gene, ligating human prourokinase cDNA with the promoter part and the intron 1 part of the gene, and then inserting the obtained plasmid into a suitable vector. A transgenic mouse prepared by using this recombinant vector secreted a considerable amount of human prourokinase as useful protein in its milk after delivery.
摘要翻译： 适用于工业制造动物的重组载体通过从牛肝中提取高分子量DNA以制备染色体基因文库，制备对应于公知牛αS1酪蛋白的合成DNA探针而在乳中产生有用的蛋白质 染色体基因，筛选携带牛αS1酪蛋白染色体基因的克隆，证实该克隆具有与公知的牛α1S酪蛋白染色体基因一致的碱基序列，将人类prourokinase cDNA与启动子部分和内含子1部分连接 该基因，然后将获得的质粒插入合适的载体中。 通过使用该重组载体制备的转基因小鼠在分娩后在其牛奶中分泌大量的人类尿激酶作为有用的蛋白质。
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