公开(公告)号：EP2891718A4

公开(公告)日：2016-03-23

申请号：EP13833253

申请日：2013-08-30

申请人： KYOWA MEDEX CO LTD

发明人： SASAKI RIE ,  KATAYAMA YUKI

IPC分类号： C12Q1/44 ,  C12Q1/26 ,  C12Q1/32 ,  C12Q1/60 ,  G01N33/92

CPC分类号： C12Q1/60 ,  C12Q1/26 ,  C12Q1/32 ,  C12Q1/44 ,  G01N33/92 ,  G01N2333/902 ,  G01N2333/904 ,  G01N2333/914 ,  G01N2333/918

摘要： Provided is a method for measuring cholesterol in high-density lipoprotein (HDL) simply and accurately. A method for measuring cholesterol in high-density lipoprotein in a specimen, comprising reacting the specimen with i) a cholesterol ester hydrolase and a cholesterol oxidase, or ii) a cholesterol ester hydrolase, an oxidized coenzyme, and a cholesterol dehydrogenase, in an aqueous medium comprising at least one substance selected from the group consisting of pyridinium salts and quaternary ammonium salts each having a specific structure, and a polyanion to form hydrogen peroxide or reduced coenzyme and measuring the formed hydrogen peroxide or reduced coenzyme.