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    METHOD FOR ASSAYING FIBROBLAST GROWTH FACTOR-23 AND ASSAY REAGENT
    1.
    发明公开
    METHOD FOR ASSAYING FIBROBLAST GROWTH FACTOR-23 AND ASSAY REAGENT 有权
    PROCEDURE用于确定所述成纤维细胞生长因子23和ENSURE测定试剂

    公开(公告)号：EP2613146A1

    公开(公告)日：2013-07-10

    申请号：EP11821852.8

    申请日：2011-08-31

    申请人： KYOWA MEDEX CO., LTD.

    发明人： UZAWA, Koji ,  SUZUKI, Emiko ,  IKEDA, Kazuyuki ,  MORITA, Kazuki

    IPC分类号： G01N33/53 ,  G01N33/543

    CPC分类号： G01N33/74 ,  G01N33/54326 ,  G01N2333/50

    摘要： A method for measuring fibroblast growth factor-23 (FGF-23) in a sample, which comprise the following steps:
    (1) reacting, in an aqueous medium, FGF-23 in a sample with magnetic particles, a first antibody or a fragment thereof which binds to FGF-23, and a second antibody or a fragment thereof which binds to FGF-23, to form on the magnetic particles an immunocomplex comprising the first antibody or a fragment thereof which binds to FGF-23, FGF-23, and the second antibody or a fragment thereof which binds to FGF-23;
    (2) collecting the magnetic particles in the reaction mixture after step (1) by magnetic force, and separating the magnetic particles collected by magnetic force from the other components; and
    (3) measuring the immunocomplex on the magnetic particles separated in step (2).
    The present invention provides a method for measuring FGF-23 in a sample, which have a high sensitivity and have a wide measurement range.

    METHOD FOR MEASURING FIBROBLAST GROWTH FACTOR-23 AND REAGENT THEREFOR
    4.
    发明公开
    METHOD FOR MEASURING FIBROBLAST GROWTH FACTOR-23 AND REAGENT THEREFOR 审中-公开
    VERFAHREN ZUR MESSUNG VON FIBROBLASTENWACHSTUMSFAKTOR-23 UND REAGENSDAFÜR

    公开(公告)号：EP3101423A1

    公开(公告)日：2016-12-07

    申请号：EP16176905.4

    申请日：2011-08-31

    申请人： KYOWA MEDEX CO., LTD.

    发明人： UZAWA, Koji ,  SUZUKI, Emiko ,  IKEDA, Kazuyuki ,  MORITA, Kazuki

    IPC分类号： G01N33/543 ,  G01N33/74

    CPC分类号： G01N33/74 ,  G01N33/54326 ,  G01N2333/50

    摘要： A method for measuring fibroblast growth factor-23 (FGF-23) in a sample, which comprise the following steps:
    (1) reacting, in an aqueous medium, FGF-23 in a sample with magnetic particles, a first antibody or a fragment thereof which binds to FGF-23, and a second antibody or a fragment thereof which binds to FGF-23, to form on the magnetic particles an immunocomplex comprising the first antibody or a fragment thereof which binds to FGF-23, FGF-23, and the second antibody or a fragment thereof which binds to FGF-23;
    (2) collecting the magnetic particles in the reaction mixture after step (1) by magnetic force, and separating the magnetic particles collected by magnetic force from the other components; and
    (3) measuring the immunocomplex on the magnetic particles separated in step (2).
    The present invention provides a method for measuring FGF-23 in a sample, which have a high sensitivity and have a wide measurement range.

    摘要翻译： （1）在水性介质中使样品中的FGF-23与磁性颗粒，第一抗体或片段（FGF-23）在一个样品中测量成纤维细胞生长因子-23（FGF-23） 其与FGF-23结合，以及结合FGF-23的第二抗​​体或其片段，在磁性颗粒上形成包含与FGF-23，FGF-23结合的第一抗体或其片段的免疫复合物， 和与FGF-23结合的第二抗体或其片段; （2）通过磁力在步骤（1）之后收集反应混合物中的磁性颗粒，并将通过磁力收集的磁性颗粒与其它组分分离; 和（3）测量在步骤（2）中分离的磁性颗粒上的免疫复合物。 本发明提供了一种用于测量样品中的FGF-23的方法，其具有高灵敏度并且具有宽的测量范围。