公开(公告)号：EP1726637A1

公开(公告)日：2006-11-29

申请号：EP05719893.9

申请日：2005-03-03

申请人： Kaneka Corporation

发明人： OKUBO, Yuji ,  MATSUMOTO, Keiji ,  TAKAGI, Masamichi ,  OHTA, Akinori

IPC分类号： C12N1/19 ,  C12N15/09 ,  C12P7/62

CPC分类号： C12N9/1025 ,  C12N9/0006 ,  C12N9/1096 ,  C12N9/88 ,  C12N9/93 ,  C12P7/625

摘要： The present invention provides a process for producing yeast excellent in cell productivity and gene manipulation of which is easy, being added with nutritional requirement by disrupting only a specific gene, and a transformant thereof. Moreover, the present invention also provides a process for producing a gene expression product, particularly a polyhydroxyalkanoic acid.
In the present invention, yeast in which a plurality of genes is disrupted is produced using the homologous recombination. Moreover, a transformant is obtained by introducing a plurality of enzyme genes involved with polyhydroxyalkanoic acid synthesis such as a polyhydroxyalkanoic acid synthase gene and an acetoacetyl CoA reductase gene into said gene-disrupted yeast. Furthermore, said transformant is cultured, copolyesters comprising a polyhydroxyalkanoic acid are efficiently accumulated within the cells, and a polymer is harvested from the cultured product.

摘要翻译： 本发明提供一种生产酵母的方法，其中容易的细胞生产力和基因操作优良，通过仅破坏特定基因而加入营养需求及其转化体。 此外，本发明还提供了生产基因表达产物，特别是聚羟基链烷酸的方法。 在本发明中，使用同源重组产生多个基因被破坏的酵母。 此外，通过将涉及聚羟基链烷酸合成的多种酶基因如聚羟基链烷酸合酶基因和乙酰乙酰辅酶A还原酶基因引入所述基因破坏的酵母中来获得转化体。 此外，培养所述转化体，包含聚羟基链烷酸的共聚酯有效地积聚在细胞内，并从培养产物中收获聚合物。

公开(公告)号：EP1626087A1

公开(公告)日：2006-02-15

申请号：EP04733092.3

申请日：2004-05-14

申请人： KANEKA CORPORATION

发明人： OKUBO, Yuji ,  NAGAOKA, Tetsuya ,  YOKOMIZO, Satoru ,  MATSUMOTO, Keiji ,  TAKAGI, Masamichi ,  OHTA, Akinori

IPC分类号： C12N15/52 ,  C12N1/19 ,  C12P7/62 ,  C12R1/72

CPC分类号： C12N9/1025 ,  C12P7/625

摘要： This invention relates to provide a gene expression cassette, which comprises a gene coding for an Aeromonas caviae-derived polyhydroxyalkanoic acid (PHA) synthase wherein a mutation has been introduced, and a promoter and a terminator both capable of functioning in yeast; a transformant wherein said gene expression cassette has been introduced in yeast, and a method for producing polyester by using said transformant.
This invention enabled a production of copolyesters resulting from copolymerization of a 3-hydroxyalkanoic acid(s) and having biodegradability and good physical properties in yeast efficiently.

摘要翻译： 本发明涉及提供一种基因表达盒，其包含编码引入突变的气单胞菌来源的聚羟基链烷酸（PHA）合酶的基因，以及能够在酵母中起作用的启动子和终止子; 其中所述基因表达盒已经引入酵母中的转化体，以及通过使用所述转化体生产聚酯的方法。 本发明能够生产由3-羟基烷酸共聚得到的共聚酯，并且在酵母中具有良好的生物降解性和良好的物理性能。