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    NOVEL BACILLUS SUBTILIS MUTANT STRAIN
    1.
    发明公开
    NOVEL BACILLUS SUBTILIS MUTANT STRAIN 有权
    中性粒细胞亚型

    公开(公告)号：EP1944365A1

    公开(公告)日：2008-07-16

    申请号：EP06798311.4

    申请日：2006-09-25

    申请人： Kao Corporation ,  Nara Institute of Science and Technology

    发明人： KADOYA, Ryosuke ,  ENDO, Keiji ,  TOHATA, Masatoshi ,  ARA, Katsutoshi ,  OGASAWARA, Naotake

    IPC分类号： C12N15/09 ,  C12N1/21

    CPC分类号： C12N15/75 ,  C12N9/2417 ,  C12N9/54 ,  C12P21/02

    摘要： Novel Bacillus subtilis mutant strains having good productivity of various enzymes are provided through extensive analysis of strains that are derived from Bacillus subtilis via gene disruption. The Bacillus subtilis mutant strains according to the present invention have genomic structures prepared by deletion of regions listed in the columns for deficient regions. Each of these Bacillus subtilis mutant strains exerts significantly improved secretory productivity of a protein when a gene encoding such a secretory target protein is introduced so that it can be expressed, compared with a case in which the same gene is introduced into a wild-type strain.

    摘要翻译： 通过对通过基因破坏衍生自枯草芽孢杆菌的菌株的广泛分析，提供具有各种酶的良好生产力的新型枯草芽孢杆菌突变菌株。 根据本发明的枯草芽孢杆菌突变株具有通过缺失缺失区域的列中列出的区域而制备的基因组结构。 当与将相同基因导入野生型菌株的情况相比，当引入编码这种分泌型靶蛋白的基因以使其能够表达时，这些枯草芽孢杆菌突变菌株中的每一种都能显着提高蛋白质的分泌生产力 。

    MUTANT PROTEASE
    4.
    发明公开
    MUTANT PROTEASE 审中-公开

    公开(公告)号：EP3940050A1

    公开(公告)日：2022-01-19

    申请号：EP20771003.9

    申请日：2020-03-06

    申请人： Kao Corporation

    发明人： TOHATA, Masatoshi

    IPC分类号： C11D3/386 ,  C12N5/10 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N15/57 ,  C12N9/54

    摘要： Provided is a mutant protease with improved stability under acidic condition. A mutant protease that consists of the amino acid sequence as shown in SEQ ID NO:2 or an amino acid sequence having an identity of at least 90% thereto, in which the amino acid residue at a position corresponding to position 303 in the amino acid sequence as shown in SEQ ID NO:2 is substituted with another amino acid residue.

    HOST MICROORGANISMS
    6.
    发明授权
    HOST MICROORGANISMS 有权
    宿主微生物

    公开(公告)号：EP1391502B1

    公开(公告)日：2012-04-25

    申请号：EP02728174.0

    申请日：2002-05-28

    申请人： KAO CORPORATION

    发明人： SAWADA, Kazuhisa ,  ENDO, Keiji ,  OZAWA, Tadahiro ,  TOHATA, Masatoshi ,  OZAKI, Katsuya

    IPC分类号： C12N1/21 ,  C12N15/09 ,  C12N9/42 ,  C12R1/07 ,  C12R1/125

    CPC分类号： C07K14/32 ,  C07K2319/00 ,  C12N1/20

    摘要： A microorganism wherein one or more genes selected from among a group of genes participating in sporulation in the medium to latter stages of sporulation have been deleted or inactivated; and a process for producing a target product (a protein) using the microorganism. No spore is formed by using this microorganism, which makes it possible to produce a target product (a protein) while relieving energy loss, suppressing the production of a by-product and a decrease in the specific production speed, and largely saving unnecessary consumption of a medium. Moreover, the production period can be prolonged thereby and thus the target product (the protein) can be efficiently produced.

    HOST MICROORGANISMS
    8.
    发明公开
    HOST MICROORGANISMS 有权
    WIRTSMIKROORGANISMEN

    公开(公告)号：EP1391502A1

    公开(公告)日：2004-02-25

    申请号：EP02728174.0

    申请日：2002-05-28

    申请人： Kao Corporation

    发明人： SAWADA, Kazuhisa ,  ENDO, Keiji ,  OZAWA, Tadahiro ,  TOHATA, Masatoshi ,  OZAKI, Katsuya

    IPC分类号： C12N1/21 ,  C12N15/09 ,  C12N9/42

    CPC分类号： C07K14/32 ,  C07K2319/00 ,  C12N1/20

    摘要： A microorganism wherein one or more genes selected from the group of genes participating in sporulation in the middle to late stages of sporulation have been deleted or inactivated; and a process for producing a target product (a protein) by use the microorganism. No spore is formed when this microorganism is employed, thereby enabling production of a target product (a protein) while decreasing energy loss, production of a by-product and specific production speed to decrease unnecessary consumption of a medium. Moreover, the production period can be prolonged, whereby the target product (the protein) can be produced efficiently.

    摘要翻译： 一种微生物，其中一个或多个选自参与孢子形成中期至晚期孢子形成的基因的基因已被删除或失活; 以及通过使用微生物生产目标产物（蛋白质）的方法。 当使用该微生物时不形成孢子，从而能够减少能量损失，副产物的产生和特定的生产速度，从而能够生产目标产物（蛋白质），从而减少不必要的培养基消耗。 此外，可以延长生产周期，由此有效地生产目标产物（蛋白质）。

    DETERGENT FOR CORNEUM-DERIVED STAINS, AND METHOD FOR EVALUATING ABILITY TO DEGRADE CORNEUM-DERIVED STAINS
    9.
    发明公开
    DETERGENT FOR CORNEUM-DERIVED STAINS, AND METHOD FOR EVALUATING ABILITY TO DEGRADE CORNEUM-DERIVED STAINS 审中-公开

    公开(公告)号：EP3741857A1

    公开(公告)日：2020-11-25

    申请号：EP19741861.9

    申请日：2019-01-15

    申请人： Kao Corporation

    发明人： YAMASHITA, Daichi ,  HIOKI, Takahiro ,  TOHATA, Masatoshi

    IPC分类号： C12N15/57 ,  C11D3/386 ,  C12N9/48 ,  C12R1/125

    摘要： The present invention provides a detergent having a high detergency against corneum-derived stains, a method for evaluating the ability of an enzyme or a composition comprising the enzyme to degrade corneum-derived stains. A detergent comprising an M23A subfamily protease as an active ingredient. A method for evaluating the ability of an enzyme or a composition containing the enzyme to degrade corneum-derived stains, comprising obtaining the degrading activities of the test enzyme or the composition comprising the test enzyme on a reference peptide and one or more substrate peptides and evaluating the ability of the test enzyme or the composition comprising the test enzyme to degrade corneum-derived stains based on the degrading activities of the test enzyme or the composition comprising the test enzyme on the reference peptide and the substrate peptide, wherein the reference peptide is GGGGG or GGGG, the one or more substrate peptides are selected from the group consisting of GGGXG, GXGGG, GGXG, and GXGG, and X is any amino acid residue other than glycine.